Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P57291 (HPRT_BUCAI) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Hypoxanthine phosphoribosyltransferase

Short name=HPRT
EC=2.4.2.8
Gene names
Name:hpt
Ordered Locus Names:BU195
OrganismBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium) [Reference proteome] [HAMAP]
Taxonomic identifier107806 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera

Protein attributes

Sequence length178 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Acts preferentially on hypoxanthine; has very low activity towards guanine. Inactive towards xanthine By similarity.

Catalytic activity

IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate.

Cofactor

Binds 2 magnesium ions per subunit. The magnesium ions are essentially bound to the substrate and have few direct interactions with the protein By similarity.

Pathway

Purine metabolism; IMP biosynthesis via salvage pathway; IMP from hypoxanthine: step 1/1.

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the purine/pyrimidine phosphoribosyltransferase family.

Sequence caution

The sequence BAB12912.1 differs from that shown. Reason: Erroneous termination at position 167. Translated as Tyr.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 178178Hypoxanthine phosphoribosyltransferase
PRO_0000139630

Regions

Nucleotide binding99 – 10810IMP By similarity
Nucleotide binding159 – 1602IMP By similarity

Sites

Active site1031Proton acceptor By similarity
Metal binding1601Magnesium By similarity
Binding site1311IMP By similarity

Sequences

Sequence LengthMass (Da)Tools
P57291 [UniParc].

Last modified January 9, 2007. Version 2.
Checksum: D79B7A06E5E2C350

FASTA17820,593
        10         20         30         40         50         60 
MKHTLQVIIT EKELDIRVRE LGQEITKKYR NSRNKMILIA LLRGSFIFIS DLCRRIHIEH 

        70         80         90        100        110        120 
EIDFMTTSSY GRGMLSSGDV KIIKDLDEDI YNKNVLIVED IIDSGKTLSK VLDILKLRNP 

       130        140        150        160        170 
KSLSICTLLD KPECREVNIN VDFIGFSILE NFFIVGYGID YAQSYRYLPN IGKVVFKK 

« Hide

References

[1]"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: APS.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000003 Genomic DNA. Translation: BAB12912.1. Sequence problems.
RefSeqNP_240026.1. NC_002528.1.

3D structure databases

ProteinModelPortalP57291.
SMRP57291. Positions 1-176.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAB12912; BAB12912; BAB12912.
GeneID1109638.
KEGGbuc:BU195.
PATRIC21243908. VBIBucAph127364_0206.

Phylogenomic databases

eggNOGCOG0634.
HOGENOMHOG000236520.
KOK00760.
OMAVDFMTVS.
OrthoDBEOG693GNP.

Enzyme and pathway databases

BioCycBAPH107806:GBZJ-194-MONOMER.
UniPathwayUPA00591; UER00648.

Family and domain databases

Gene3D3.40.50.2020. 1 hit.
InterProIPR005904. Hxn_phspho_trans.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamPF00156. Pribosyltran. 1 hit.
[Graphical view]
SUPFAMSSF53271. SSF53271. 1 hit.
TIGRFAMsTIGR01203. HGPRTase. 1 hit.
PROSITEPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameHPRT_BUCAI
AccessionPrimary (citable) accession number: P57291
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: January 9, 2007
Last modified: June 11, 2014
This is version 85 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Buchnera aphidicola (subsp. Acyrthosiphon pisum)

Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names