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Protein

Hypoxanthine phosphoribosyltransferase

Gene

hpt

Organism
Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Acts preferentially on hypoxanthine; has very low activity towards guanine. Inactive towards xanthine (By similarity).By similarity

Catalytic activityi

IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate.

Cofactori

Mg2+By similarityNote: Binds 2 magnesium ions per subunit. The magnesium ions are essentially bound to the substrate and have few direct interactions with the protein.By similarity

Pathwayi: IMP biosynthesis via salvage pathway

This protein is involved in step 1 of the subpathway that synthesizes IMP from hypoxanthine.
Proteins known to be involved in this subpathway in this organism are:
  1. Hypoxanthine phosphoribosyltransferase (hpt)
This subpathway is part of the pathway IMP biosynthesis via salvage pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes IMP from hypoxanthine, the pathway IMP biosynthesis via salvage pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei103Proton acceptorBy similarity1
Binding sitei131IMPBy similarity1
Metal bindingi160MagnesiumBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi99 – 108IMPBy similarity10
Nucleotide bindingi159 – 160IMPBy similarity2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase
Biological processPurine salvage
LigandMagnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00591; UER00648.

Names & Taxonomyi

Protein namesi
Recommended name:
Hypoxanthine phosphoribosyltransferase (EC:2.4.2.8)
Short name:
HPRT
Gene namesi
Name:hpt
Ordered Locus Names:BU195
OrganismiBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Taxonomic identifieri107806 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesErwiniaceaeBuchnera
Proteomesi
  • UP000001806 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001396301 – 178Hypoxanthine phosphoribosyltransferaseAdd BLAST178

Interactioni

Protein-protein interaction databases

STRINGi107806.BU195.

Structurei

3D structure databases

ProteinModelPortaliP57291.
SMRiP57291.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4107XRV. Bacteria.
COG0634. LUCA.
HOGENOMiHOG000236520.
KOiK00760.
OMAiRNIMDDL.

Family and domain databases

CDDicd06223. PRTases_typeI. 1 hit.
InterProiView protein in InterPro
IPR005904. Hxn_phspho_trans.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
PfamiView protein in Pfam
PF00156. Pribosyltran. 1 hit.
SUPFAMiSSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR01203. HGPRTase. 1 hit.
PROSITEiView protein in PROSITE
PS00103. PUR_PYR_PR_TRANSFER. 1 hit.

Sequencei

Sequence statusi: Complete.

P57291-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKHTLQVIIT EKELDIRVRE LGQEITKKYR NSRNKMILIA LLRGSFIFIS
60 70 80 90 100
DLCRRIHIEH EIDFMTTSSY GRGMLSSGDV KIIKDLDEDI YNKNVLIVED
110 120 130 140 150
IIDSGKTLSK VLDILKLRNP KSLSICTLLD KPECREVNIN VDFIGFSILE
160 170
NFFIVGYGID YAQSYRYLPN IGKVVFKK
Length:178
Mass (Da):20,593
Last modified:January 9, 2007 - v2
Checksum:iD79B7A06E5E2C350
GO

Sequence cautioni

The sequence BAB12912 differs from that shown. Reason: Erroneous termination at position 167. Translated as Tyr.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000003 Genomic DNA. Translation: BAB12912.1. Sequence problems.
RefSeqiNP_240026.1. NC_002528.1.
WP_010895994.1. NC_002528.1.

Genome annotation databases

EnsemblBacteriaiBAB12912; BAB12912; BAB12912.
GeneIDi1109638.
KEGGibuc:BU195.
PATRICifig|107806.10.peg.206.

Similar proteinsi

Entry informationi

Entry nameiHPRT_BUCAI
AccessioniPrimary (citable) accession number: P57291
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: January 9, 2007
Last modified: August 30, 2017
This is version 106 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Buchnera aphidicola (subsp. Acyrthosiphon pisum)
    Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families