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P57290

- AKH_BUCAI

UniProt

P57290 - AKH_BUCAI

Protein

Bifunctional aspartokinase/homoserine dehydrogenase

Gene

thrA

Organism
Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 98 (01 Oct 2014)
      Sequence version 1 (01 Dec 2000)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    L-homoserine + NAD(P)+ = L-aspartate 4-semialdehyde + NAD(P)H.
    ATP + L-aspartate = ADP + 4-phospho-L-aspartate.

    Pathwayi

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi472 – 4798NADPSequence Analysis

    GO - Molecular functioni

    1. amino acid binding Source: InterPro
    2. aspartate kinase activity Source: UniProtKB-EC
    3. ATP binding Source: UniProtKB-KW
    4. homoserine dehydrogenase activity Source: UniProtKB-EC
    5. NADP binding Source: InterPro

    GO - Biological processi

    1. lysine biosynthetic process via diaminopimelate Source: UniProtKB-UniPathway
    2. threonine biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Kinase, Oxidoreductase, Transferase

    Keywords - Biological processi

    Amino-acid biosynthesis, Threonine biosynthesis

    Keywords - Ligandi

    ATP-binding, NADP, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciBAPH107806:GBZJ-193-MONOMER.
    UniPathwayiUPA00034; UER00015.
    UPA00050; UER00063.
    UPA00050; UER00461.
    UPA00051; UER00462.
    UPA00051; UER00465.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Bifunctional aspartokinase/homoserine dehydrogenase
    Short name:
    AK-HD
    Including the following 2 domains:
    Aspartokinase (EC:2.7.2.4)
    Homoserine dehydrogenase (EC:1.1.1.3)
    Gene namesi
    Name:thrA
    Ordered Locus Names:BU194
    OrganismiBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
    Taxonomic identifieri107806 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
    ProteomesiUP000001806: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 816816Bifunctional aspartokinase/homoserine dehydrogenasePRO_0000066684Add
    BLAST

    Proteomic databases

    PRIDEiP57290.

    Interactioni

    Subunit structurei

    Homotetramer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP57290.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini402 – 47978ACTPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 250250AspartokinaseBy similarityAdd
    BLAST
    Regioni251 – 471221InterfaceBy similarityAdd
    BLAST
    Regioni472 – 816345Homoserine dehydrogenaseBy similarityAdd
    BLAST

    Sequence similaritiesi

    In the N-terminal section; belongs to the aspartokinase family.Curated
    In the C-terminal section; belongs to the homoserine dehydrogenase family.Curated
    Contains 1 ACT domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0527.
    HOGENOMiHOG000271593.
    KOiK12524.
    OMAiYIFGMLD.
    OrthoDBiEOG6M0T34.

    Family and domain databases

    Gene3Di3.40.1160.10. 2 hits.
    3.40.50.720. 1 hit.
    InterProiIPR002912. ACT_dom.
    IPR001048. Asp/Glu/Uridylate_kinase.
    IPR005106. Asp/hSer_DH_NAD-bd.
    IPR001341. Asp_kinase_dom.
    IPR018042. Aspartate_kinase_CS.
    IPR011147. Bifunc_aspartokin/hSer_DH.
    IPR001342. HDH_cat.
    IPR019811. HDH_CS.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PfamiPF00696. AA_kinase. 1 hit.
    PF00742. Homoserine_dh. 1 hit.
    PF03447. NAD_binding_3. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000727. ThrA. 1 hit.
    SUPFAMiSSF53633. SSF53633. 1 hit.
    TIGRFAMsiTIGR00657. asp_kinases. 1 hit.
    PROSITEiPS51671. ACT. 1 hit.
    PS00324. ASPARTOKINASE. 1 hit.
    PS01042. HOMOSER_DHGENASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P57290-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKLLKFGGTS LANAEKFLSV SSIIEENTQT DQIAVVLSAP AKITNYLVKI    50
    IENTIKNNQI LETVHLAENI FMQLINNFLN IQSNFPHKEI EKIIKKEFNE 100
    LKNIIQGILL LKQCPDNIRA IIISRGEILS VFIMKSILQS KNYNVTIINP 150
    VKNLVAIGDN YLDSTVDISE SKKNIQNMNI NQSNIILMAG FIAGNKDKKL 200
    VVLGRNGSDY SAAVLAACLD ANCCEIWTDV DGVFTSDPRK VPNARLLKSI 250
    SYQEAMELSY FGAKVLHPRT IEPIAQFKIP CLIKNTNNVK SIGTLICEQN 300
    CSEKDFLKGV THLDEIAMFN ISGPHIKDVG SVISRIFTMM SRGNIKILLI 350
    TQSSSENKIN FCVYEHDIYK ILYLFNKEFQ LELKDGLLNP FKIKKNLSIL 400
    SIVGSNIYKK HNIASKIFSV LGALKINVIA ISQGSSKHSI SLVIKKENIL 450
    KAVQHVHNTL FFNKKTIHMF LIGIGGIGST LLNQILKQKQ FLDKKNIEIK 500
    ICAIANSKKI LLLDNTNDLS NWKNDFKKST QKFNLELLNN LIKNNHFSNS 550
    VIVDCTSSQL LSEQYVNFID NNFHVVTSNK KANTSEWNYY KKIRKSVAQT 600
    GKKFLYETNV GAGLPVIETL QNLFKSGDNL ICFKGILSGS LSFIFGRLEE 650
    GILLSQATRE AKELGFTEPN PCDDLSGIDV ARKLLILARE SGYNIELKDI 700
    KIEPLLPNNF KIYEDTDKFL LKLKELDVYF SNKIKQALNV GNVLRFVATI 750
    EQKRQFFIKL EEVNINNPLY KVKNGENALA FYTNYYQPIP LVLRGYGAGN 800
    NVTASGVFSD LLRTLS 816
    Length:816
    Mass (Da):91,809
    Last modified:December 1, 2000 - v1
    Checksum:iCEACE5C565465884
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000003 Genomic DNA. Translation: BAB12911.1.
    RefSeqiNP_240025.1. NC_002528.1.

    Genome annotation databases

    EnsemblBacteriaiBAB12911; BAB12911; BAB12911.
    GeneIDi1109637.
    KEGGibuc:BU194.
    PATRICi21243906. VBIBucAph127364_0205.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000003 Genomic DNA. Translation: BAB12911.1 .
    RefSeqi NP_240025.1. NC_002528.1.

    3D structure databases

    ProteinModelPortali P57290.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi P57290.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAB12911 ; BAB12911 ; BAB12911 .
    GeneIDi 1109637.
    KEGGi buc:BU194.
    PATRICi 21243906. VBIBucAph127364_0205.

    Phylogenomic databases

    eggNOGi COG0527.
    HOGENOMi HOG000271593.
    KOi K12524.
    OMAi YIFGMLD.
    OrthoDBi EOG6M0T34.

    Enzyme and pathway databases

    UniPathwayi UPA00034 ; UER00015 .
    UPA00050 ; UER00063 .
    UPA00050 ; UER00461 .
    UPA00051 ; UER00462 .
    UPA00051 ; UER00465 .
    BioCyci BAPH107806:GBZJ-193-MONOMER.

    Family and domain databases

    Gene3Di 3.40.1160.10. 2 hits.
    3.40.50.720. 1 hit.
    InterProi IPR002912. ACT_dom.
    IPR001048. Asp/Glu/Uridylate_kinase.
    IPR005106. Asp/hSer_DH_NAD-bd.
    IPR001341. Asp_kinase_dom.
    IPR018042. Aspartate_kinase_CS.
    IPR011147. Bifunc_aspartokin/hSer_DH.
    IPR001342. HDH_cat.
    IPR019811. HDH_CS.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    Pfami PF00696. AA_kinase. 1 hit.
    PF00742. Homoserine_dh. 1 hit.
    PF03447. NAD_binding_3. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000727. ThrA. 1 hit.
    SUPFAMi SSF53633. SSF53633. 1 hit.
    TIGRFAMsi TIGR00657. asp_kinases. 1 hit.
    PROSITEi PS51671. ACT. 1 hit.
    PS00324. ASPARTOKINASE. 1 hit.
    PS01042. HOMOSER_DHGENASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
      Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
      Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: APS.

    Entry informationi

    Entry nameiAKH_BUCAI
    AccessioniPrimary (citable) accession number: P57290
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: December 1, 2000
    Last modified: October 1, 2014
    This is version 98 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. Buchnera aphidicola (subsp. Acyrthosiphon pisum)
      Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3