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P57290

- AKH_BUCAI

UniProt

P57290 - AKH_BUCAI

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Protein

Bifunctional aspartokinase/homoserine dehydrogenase

Gene
thrA, BU194
Organism
Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Catalytic activityi

L-homoserine + NAD(P)+ = L-aspartate 4-semialdehyde + NAD(P)H.
ATP + L-aspartate = ADP + 4-phospho-L-aspartate.

Pathwayi

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi472 – 4798NADP Reviewed prediction

GO - Molecular functioni

  1. amino acid binding Source: InterPro
  2. aspartate kinase activity Source: UniProtKB-EC
  3. ATP binding Source: UniProtKB-KW
  4. homoserine dehydrogenase activity Source: UniProtKB-EC
  5. NADP binding Source: InterPro

GO - Biological processi

  1. lysine biosynthetic process via diaminopimelate Source: UniProtKB-UniPathway
  2. threonine biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Oxidoreductase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Threonine biosynthesis

Keywords - Ligandi

ATP-binding, NADP, Nucleotide-binding

Enzyme and pathway databases

BioCyciBAPH107806:GBZJ-193-MONOMER.
UniPathwayiUPA00034; UER00015.
UPA00050; UER00063.
UPA00050; UER00461.
UPA00051; UER00462.
UPA00051; UER00465.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional aspartokinase/homoserine dehydrogenase
Short name:
AK-HD
Including the following 2 domains:
Aspartokinase (EC:2.7.2.4)
Homoserine dehydrogenase (EC:1.1.1.3)
Gene namesi
Name:thrA
Ordered Locus Names:BU194
OrganismiBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Taxonomic identifieri107806 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
ProteomesiUP000001806: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 816816Bifunctional aspartokinase/homoserine dehydrogenasePRO_0000066684Add
BLAST

Proteomic databases

PRIDEiP57290.

Interactioni

Subunit structurei

Homotetramer By similarity.

Structurei

3D structure databases

ProteinModelPortaliP57290.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini402 – 47978ACTAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 250250Aspartokinase By similarityAdd
BLAST
Regioni251 – 471221Interface By similarityAdd
BLAST
Regioni472 – 816345Homoserine dehydrogenase By similarityAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the aspartokinase family.
In the C-terminal section; belongs to the homoserine dehydrogenase family.
Contains 1 ACT domain.

Phylogenomic databases

eggNOGiCOG0527.
HOGENOMiHOG000271593.
KOiK12524.
OMAiYIFGMLD.
OrthoDBiEOG6M0T34.

Family and domain databases

Gene3Di3.40.1160.10. 2 hits.
3.40.50.720. 1 hit.
InterProiIPR002912. ACT_dom.
IPR001048. Asp/Glu/Uridylate_kinase.
IPR005106. Asp/hSer_DH_NAD-bd.
IPR001341. Asp_kinase_dom.
IPR018042. Aspartate_kinase_CS.
IPR011147. Bifunc_aspartokin/hSer_DH.
IPR001342. HDH_cat.
IPR019811. HDH_CS.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00696. AA_kinase. 1 hit.
PF00742. Homoserine_dh. 1 hit.
PF03447. NAD_binding_3. 1 hit.
[Graphical view]
PIRSFiPIRSF000727. ThrA. 1 hit.
SUPFAMiSSF53633. SSF53633. 1 hit.
TIGRFAMsiTIGR00657. asp_kinases. 1 hit.
PROSITEiPS51671. ACT. 1 hit.
PS00324. ASPARTOKINASE. 1 hit.
PS01042. HOMOSER_DHGENASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P57290-1 [UniParc]FASTAAdd to Basket

« Hide

MKLLKFGGTS LANAEKFLSV SSIIEENTQT DQIAVVLSAP AKITNYLVKI    50
IENTIKNNQI LETVHLAENI FMQLINNFLN IQSNFPHKEI EKIIKKEFNE 100
LKNIIQGILL LKQCPDNIRA IIISRGEILS VFIMKSILQS KNYNVTIINP 150
VKNLVAIGDN YLDSTVDISE SKKNIQNMNI NQSNIILMAG FIAGNKDKKL 200
VVLGRNGSDY SAAVLAACLD ANCCEIWTDV DGVFTSDPRK VPNARLLKSI 250
SYQEAMELSY FGAKVLHPRT IEPIAQFKIP CLIKNTNNVK SIGTLICEQN 300
CSEKDFLKGV THLDEIAMFN ISGPHIKDVG SVISRIFTMM SRGNIKILLI 350
TQSSSENKIN FCVYEHDIYK ILYLFNKEFQ LELKDGLLNP FKIKKNLSIL 400
SIVGSNIYKK HNIASKIFSV LGALKINVIA ISQGSSKHSI SLVIKKENIL 450
KAVQHVHNTL FFNKKTIHMF LIGIGGIGST LLNQILKQKQ FLDKKNIEIK 500
ICAIANSKKI LLLDNTNDLS NWKNDFKKST QKFNLELLNN LIKNNHFSNS 550
VIVDCTSSQL LSEQYVNFID NNFHVVTSNK KANTSEWNYY KKIRKSVAQT 600
GKKFLYETNV GAGLPVIETL QNLFKSGDNL ICFKGILSGS LSFIFGRLEE 650
GILLSQATRE AKELGFTEPN PCDDLSGIDV ARKLLILARE SGYNIELKDI 700
KIEPLLPNNF KIYEDTDKFL LKLKELDVYF SNKIKQALNV GNVLRFVATI 750
EQKRQFFIKL EEVNINNPLY KVKNGENALA FYTNYYQPIP LVLRGYGAGN 800
NVTASGVFSD LLRTLS 816
Length:816
Mass (Da):91,809
Last modified:December 1, 2000 - v1
Checksum:iCEACE5C565465884
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000003 Genomic DNA. Translation: BAB12911.1.
RefSeqiNP_240025.1. NC_002528.1.

Genome annotation databases

EnsemblBacteriaiBAB12911; BAB12911; BAB12911.
GeneIDi1109637.
KEGGibuc:BU194.
PATRICi21243906. VBIBucAph127364_0205.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000003 Genomic DNA. Translation: BAB12911.1 .
RefSeqi NP_240025.1. NC_002528.1.

3D structure databases

ProteinModelPortali P57290.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi P57290.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAB12911 ; BAB12911 ; BAB12911 .
GeneIDi 1109637.
KEGGi buc:BU194.
PATRICi 21243906. VBIBucAph127364_0205.

Phylogenomic databases

eggNOGi COG0527.
HOGENOMi HOG000271593.
KOi K12524.
OMAi YIFGMLD.
OrthoDBi EOG6M0T34.

Enzyme and pathway databases

UniPathwayi UPA00034 ; UER00015 .
UPA00050 ; UER00063 .
UPA00050 ; UER00461 .
UPA00051 ; UER00462 .
UPA00051 ; UER00465 .
BioCyci BAPH107806:GBZJ-193-MONOMER.

Family and domain databases

Gene3Di 3.40.1160.10. 2 hits.
3.40.50.720. 1 hit.
InterProi IPR002912. ACT_dom.
IPR001048. Asp/Glu/Uridylate_kinase.
IPR005106. Asp/hSer_DH_NAD-bd.
IPR001341. Asp_kinase_dom.
IPR018042. Aspartate_kinase_CS.
IPR011147. Bifunc_aspartokin/hSer_DH.
IPR001342. HDH_cat.
IPR019811. HDH_CS.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
Pfami PF00696. AA_kinase. 1 hit.
PF00742. Homoserine_dh. 1 hit.
PF03447. NAD_binding_3. 1 hit.
[Graphical view ]
PIRSFi PIRSF000727. ThrA. 1 hit.
SUPFAMi SSF53633. SSF53633. 1 hit.
TIGRFAMsi TIGR00657. asp_kinases. 1 hit.
PROSITEi PS51671. ACT. 1 hit.
PS00324. ASPARTOKINASE. 1 hit.
PS01042. HOMOSER_DHGENASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
    Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
    Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: APS.

Entry informationi

Entry nameiAKH_BUCAI
AccessioniPrimary (citable) accession number: P57290
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: May 14, 2014
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. Buchnera aphidicola (subsp. Acyrthosiphon pisum)
    Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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