Bifunctional aspartokinase/homoserine dehydrogenase - Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS)

Preferred order of sections

Names and origin

Protein names

Recommended name:
Bifunctional aspartokinase/homoserine dehydrogenase
Short name=AK-HD

Including the following 2 domains:

Aspartokinase
EC=2.7.2.4
Homoserine dehydrogenase
EC=1.1.1.3

Gene names

Name:	thrA
Ordered Locus Names:	BU194

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium) [Reference proteome] [HAMAP]

Taxonomic identifier

107806 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera ›

Protein attributes

Sequence length	816 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	L-homoserine + NAD(P)⁺ = L-aspartate 4-semialdehyde + NAD(P)H. ATP + L-aspartate = ADP + 4-phospho-L-aspartate.
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 1/4. Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 1/3. Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 3/3. Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 1/5. Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 3/5.
Subunit structure	Homotetramer By similarity.
Sequence similarities	In the N-terminal section; belongs to the aspartokinase family. In the C-terminal section; belongs to the homoserine dehydrogenase family. Contains 1 ACT domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Threonine biosynthesis
Ligand	ATP-binding NADP Nucleotide-binding
Molecular function	Kinase Oxidoreductase Transferase
Technical term	Complete proteome Multifunctional enzyme Reference proteome
Gene Ontology (GO)
Biological_process	lysine biosynthetic process via diaminopimelate Inferred from electronic annotation. Source: UniProtKB-UniPathway threonine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW NADP binding Inferred from electronic annotation. Source: InterPro aspartate kinase activity Inferred from electronic annotation. Source: EC homoserine dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 816

816

Bifunctional aspartokinase/homoserine dehydrogenase

PRO_0000066684

Regions

Domain

398 – 468

71

ACT

Nucleotide binding

472 – 479

8

NADP Potential

Region

1 – 250

250

Aspartokinase By similarity

Region

251 – 471

221

Interface By similarity

Region

472 – 816

345

Homoserine dehydrogenase By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

P57290 [UniParc].

Last modified December 1, 2000. Version 1.
Checksum: CEACE5C565465884
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FASTA

816

91,809

        10         20         30         40         50         60 
MKLLKFGGTS LANAEKFLSV SSIIEENTQT DQIAVVLSAP AKITNYLVKI IENTIKNNQI 

        70         80         90        100        110        120 
LETVHLAENI FMQLINNFLN IQSNFPHKEI EKIIKKEFNE LKNIIQGILL LKQCPDNIRA 

       130        140        150        160        170        180 
IIISRGEILS VFIMKSILQS KNYNVTIINP VKNLVAIGDN YLDSTVDISE SKKNIQNMNI 

       190        200        210        220        230        240 
NQSNIILMAG FIAGNKDKKL VVLGRNGSDY SAAVLAACLD ANCCEIWTDV DGVFTSDPRK 

       250        260        270        280        290        300 
VPNARLLKSI SYQEAMELSY FGAKVLHPRT IEPIAQFKIP CLIKNTNNVK SIGTLICEQN 

       310        320        330        340        350        360 
CSEKDFLKGV THLDEIAMFN ISGPHIKDVG SVISRIFTMM SRGNIKILLI TQSSSENKIN 

       370        380        390        400        410        420 
FCVYEHDIYK ILYLFNKEFQ LELKDGLLNP FKIKKNLSIL SIVGSNIYKK HNIASKIFSV 

       430        440        450        460        470        480 
LGALKINVIA ISQGSSKHSI SLVIKKENIL KAVQHVHNTL FFNKKTIHMF LIGIGGIGST 

       490        500        510        520        530        540 
LLNQILKQKQ FLDKKNIEIK ICAIANSKKI LLLDNTNDLS NWKNDFKKST QKFNLELLNN 

       550        560        570        580        590        600 
LIKNNHFSNS VIVDCTSSQL LSEQYVNFID NNFHVVTSNK KANTSEWNYY KKIRKSVAQT 

       610        620        630        640        650        660 
GKKFLYETNV GAGLPVIETL QNLFKSGDNL ICFKGILSGS LSFIFGRLEE GILLSQATRE 

       670        680        690        700        710        720 
AKELGFTEPN PCDDLSGIDV ARKLLILARE SGYNIELKDI KIEPLLPNNF KIYEDTDKFL 

       730        740        750        760        770        780 
LKLKELDVYF SNKIKQALNV GNVLRFVATI EQKRQFFIKL EEVNINNPLY KVKNGENALA 

       790        800        810 
FYTNYYQPIP LVLRGYGAGN NVTASGVFSD LLRTLS

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References

[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: APS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000003 Genomic DNA. Translation: BAB12911.1.
RefSeq	NP_240025.1. NC_002528.1.
3D structure databases
ProteinModelPortal	P57290.
ModBase	Search...
Proteomic databases
PRIDE	P57290.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAB12911; BAB12911; BAB12911.
GeneID	1109637.
KEGG	buc:BU194.
PATRIC	21243906. VBIBucAph127364_0205.
Phylogenomic databases
eggNOG	COG0527.
HOGENOM	HOG000271593.
KO	K12524.
OMA	IFISQAS.
ProtClustDB	PRK09436.
Enzyme and pathway databases
BioCyc	BAPH107806:GBZJ-193-MONOMER.
UniPathway	UPA00034; UER00015. UPA00050; UER00063. UPA00050; UER00461. UPA00051; UER00462. UPA00051; UER00465.
Family and domain databases
Gene3D	3.40.1160.10. 2 hits. 3.40.50.720. 1 hit.
InterPro	IPR001048. Asp/Glu/Uridylate_kinase. IPR005106. Asp/hSer_DH_NAD-bd. IPR001341. Asp_kinase_dom. IPR018042. Aspartate_kinase_CS. IPR011147. Bifunc_aspartokin/hSer_DH. IPR001342. HDH_cat. IPR019811. HDH_CS. IPR016040. NAD(P)-bd_dom. [Graphical view]
Pfam	PF00696. AA_kinase. 1 hit. PF00742. Homoserine_dh. 1 hit. PF03447. NAD_binding_3. 1 hit. [Graphical view]
PIRSF	PIRSF000727. ThrA. 1 hit.
SUPFAM	SSF53633. Aa_kinase. 1 hit.
TIGRFAMs	TIGR00657. asp_kinases. 1 hit.
PROSITE	PS00324. ASPARTOKINASE. 1 hit. PS01042. HOMOSER_DHGENASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AKH_BUCAI

Accession

Primary (citable) accession number: P57290

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	May 29, 2013
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Buchnera aphidicola (subsp. Acyrthosiphon pisum)

Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Including the following 2 domains:

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sequences

References

Cross-references

Sequence databases

3D structure databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents