Reviewed, UniProtKB/Swiss-Prot P57287 (PTH_BUCAI)

Names and origin

Protein names

Recommended name:
    Peptidyl-tRNA hydrolase
      Short name=PTH
    EC=3.1.1.29

Gene names

Name:	pth
Ordered Locus Names:	BU190

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (Acyrthosiphon pisum symbiotic bacterium) [Complete proteome] [HAMAP]

Taxonomic identifier

118099 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	177 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	The natural substrate for this enzyme may be peptidyl-tRNAs which drop off the ribosome during protein synthesis By similarity.
Catalytic activity	N-substituted aminoacyl-tRNA + H2O = N-substituted amino acid + tRNA. HAMAP MF_00083
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the PTH family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	translation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aminoacyl-tRNA hydrolase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 177	177	Peptidyl-tRNA hydrolase HAMAP MF_00083		PRO_0000187707

Sequences

	Sequence		Length	Mass (Da)
	P57287-1 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: BD18DA8FEF0DFAAF	FASTA	177	20,576
	10 20 30 40 50 60 MIVGLSNPKK EYHSTRHNVG SWYLYSLAES YLRNFKNEKK FFGFTTSLNI ESNYIRLLIP 70 80 90 100 110 120 NIFMNINGQS VFKMASFYNI NLSEILIVHD DLELQPGISK LKYSYGHNGH NGLRDIVNTF 130 140 150 160 170 NKNINFYRFR IGIGRPINRD QIASFVLSNP TKKEKILIQK SILHAIEKNV LSNILKF

References

[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed: 10993077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tokyo 1998.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000003 Genomic DNA. Translation: BAB12907.1.
RefSeq	NP_240021.1.
3D structure databases
HSSP	HSSP built from PDB template 2PTH based on UniProtKB P23932.
ModBase	Search...
Genome annotation databases
GeneID	1109633.
GenomeReviews	Gene locus BU190 in contig BA000003_GR.
KEGG	buc:BU190.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P57287.
OMA	P57287. IKFKTGG.
Enzyme and pathway databases
BioCyc	BSP107806:BU190-MON.
Family and domain databases
HAMAP	MF_00083. [Tree]
InterPro	IPR001328. Pept_tRNA_hydro. IPR018171. Pept_tRNA_hydro_CS. [Graphical view]
Gene3D	G3DSA:3.40.50.1470. Pept_tRNA_hydro. 1 hit.
PANTHER	PTHR17224. Pept_tRNA_hydro. 1 hit.
Pfam	PF01195. Pept_tRNA_hydro. 1 hit. [Graphical view]
ProDom	PD005324. PeptRNAhydrolase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00447. pth. 1 hit.
PROSITE	PS01195. PEPT_TRNA_HYDROL_1. False negative. PS01196. PEPT_TRNA_HYDROL_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PTH_BUCAI

Accession

Primary (citable) accession number: P57287

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	June 16, 2009
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)