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P57281 (GRPE2_BUCAI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein GrpE 2
Alternative name(s):
HSP-70 cofactor 2
Gene names
Name:grpE2
Ordered Locus Names:BU184
OrganismBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium) [Reference proteome] [HAMAP]
Taxonomic identifier107806 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera

Protein attributes

Sequence length188 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with DnaK and GrpE. It is the nucleotide exchange factor for DnaK and may function as a thermosensor. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding By similarity. HAMAP-Rule MF_01151

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01151

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_01151.

Sequence similarities

Belongs to the GrpE family.

Ontologies

Keywords
   Biological processStress response
   Cellular componentCytoplasm
   Molecular functionChaperone
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processprotein folding

Inferred from electronic annotation. Source: InterPro

response to stress

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionadenyl-nucleotide exchange factor activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 188188Protein GrpE 2 HAMAP-Rule MF_01151
PRO_0000113760

Sequences

Sequence LengthMass (Da)Tools
P57281 [UniParc].

Last modified December 1, 2000. Version 1.
Checksum: 1FEC6225B582BC81

FASTA18821,726
        10         20         30         40         50         60 
MDNKENKLDE KKIFKNNKIE EKKENLIDAI TVQNQKIENL KLKLLQNQKK INDIELRKLA 

        70         80         90        100        110        120 
NIENIKKNTE EKIEKIKKTE IERFLKSIIP VIDSLEDILN LSTTVDIKDQ PIIKGIELTL 

       130        140        150        160        170        180 
ESLLNILNKL GVKIEGQKNK VFNPDIHELV SRELSKETLP NHVISVNKKG FTFNKIVLRK 


ASVIVAEN 

« Hide

References

[1]"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: APS.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000003 Genomic DNA. Translation: BAB12901.1.
RefSeqNP_240015.1. NC_002528.1.

3D structure databases

ProteinModelPortalP57281.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAB12901; BAB12901; BAB12901.
GeneID1109627.
KEGGbuc:BU184.
PATRIC21243886. VBIBucAph127364_0195.

Phylogenomic databases

eggNOGCOG0576.
HOGENOMHOG000252084.
KOK03687.
OMARTYAVAN.
OrthoDBEOG61ZTJ2.

Enzyme and pathway databases

BioCycBAPH107806:GBZJ-183-MONOMER.

Family and domain databases

Gene3D2.30.22.10. 1 hit.
3.90.20.20. 1 hit.
HAMAPMF_01151. GrpE.
InterProIPR000740. GrpE.
IPR013805. GrpE_coiled_coil.
IPR009012. GrpE_head.
[Graphical view]
PANTHERPTHR21237. PTHR21237. 1 hit.
PfamPF01025. GrpE. 1 hit.
[Graphical view]
PRINTSPR00773. GRPEPROTEIN.
SUPFAMSSF51064. SSF51064. 1 hit.
PROSITEPS01071. GRPE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGRPE2_BUCAI
AccessionPrimary (citable) accession number: P57281
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: May 14, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Buchnera aphidicola (subsp. Acyrthosiphon pisum)

Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names