P57277 (GYRA_BUCAI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA gyrase subunit A
EC=5.99.1.3

Gene names

Name:	gyrA
Ordered Locus Names:	BU180

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)

Taxonomic identifier

107806 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	830 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings. HAMAP MF_01897
Catalytic activity	ATP-dependent breakage, passage and rejoining of double-stranded DNA. HAMAP MF_01897
Subunit structure	Made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis. The enzyme forms an A2B2 tetramer.
Subcellular location	Cytoplasm Potential HAMAP MF_01897.
Sequence similarities	Belongs to the topoisomerase GyrA/ParC subunit family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Isomerase Topoisomerase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 830	830	DNA gyrase subunit A HAMAP MF_01897		PRO_0000145223
Sites
	Active site	122	1	O-(5'-phospho-DNA)-tyrosine intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P57277 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: 29B995C7B09A9073	FASTA	830	93,879
	10 20 30 40 50 60 MKDPSREIIQ VNIEEELKSS YLDYSMSVIV GRALPDVRDG LKPVHRRILF AMYILNNDWN 70 80 90 100 110 120 KAYKKSARIV GDVIGKYHPH GDSAVYDAIV RMAQKFSLRY MLIDGQGNFG SVDGDSAAAM 130 140 150 160 170 180 RYTEVRMSKI AHELLNDLEK NTVEFLPNYD GTEYIPEILP AKIPNLLING SSGIAVGMAT 190 200 210 220 230 240 NIPPHNLNEV INGCLAYIDN NDITLEELIK HIPGPDFPTA AIINGKSGIE EAYRTGKGKI 250 260 270 280 290 300 YIRAQNQIEK NKKNKKESIV FNEIPYQVNK SRLIEKIAEL VKEKRIDGIT ALRDESDKDG 310 320 330 340 350 360 MRIVIEIKRE AIAEVILNQL YSLTQLQISF GINMVALCQG QPKTLSLKEI LKNFLSHRQE 370 380 390 400 410 420 IIIRRSLFEL NKVRNRIHIL EGLNMALINI NAIIEIIKNS VNSIDAKKII IQKNWKSEKI 430 440 450 460 470 480 NYLAKKHEYY FSEKQAQAIL DLRLHKITNL EQEKIIMEHN DLIKKTKELK EILENPKKMF 490 500 510 520 530 540 EVIKSELLSI QNNFSDKRRT KITENHSDIN MEDLINQEDV VVTLSHSGYV KYQPLSDYNA 550 560 570 580 590 600 QRRGGKGKSA AKIKEEDFIE SLVIANTHDT ILCFSSRGIL YWMKVYQLPE SSRHARGRPI 610 620 630 640 650 660 VNLLPLSPKE RITAILPVHK YQDNLNIFMT TAHGIVKKSS LSQFKKPRFA GIIAINLHAN 670 680 690 700 710 720 DELIGVALTD GNNNIMLFTQ NGKVVQFLEN SVRTMGRTAS GVKGIKIKKN DKVVSLIVPK 730 740 750 760 770 780 NKGSILIATK NGYGKRTKIS DFPIKSRATQ GVISIKITKK NGKIIGAIQV IEKDQIMMIT 790 800 810 820 830 DAGTLVRIRV SEVGVLKRNT QGVILIRTSK NEKVVALQKI VDPMIEKIDL

References

[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed: 10993077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: APS.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000003 Genomic DNA. Translation: BAB12897.1.
RefSeq	NP_240011.1. NC_002528.1.
3D structure databases
ProteinModelPortal	P57277.
SMR	P57277. Positions 30-503, 517-822.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBUCT00000002778; EBBUCP00000002554; EBBUCG00000002778.
GeneID	1109623.
GenomeReviews	Gene locus BU180 in contig BA000003_GR.
KEGG	buc:BU180.
PATRIC	21243878. VBIBucAph127364_0191.
Phylogenomic databases
GeneTree	EBGT00050000007782.
HOGENOM	HBG635183.
OMA	TGRGRIY.
PhylomeDB	P57277.
ProtClustDB	PRK05560.
Enzyme and pathway databases
BioCyc	BSP107806:BU180-MONOMER.
Family and domain databases
HAMAP	MF_01897. GyrA. [Tree]
InterPro	IPR005743. GyrA. IPR006691. GyrA/parC_pinwhl. IPR002205. Topo_IIA_A/C. IPR013758. Topo_IIA_A/C_ab. IPR013757. Topo_IIA_A_a. IPR013760. Topo_IIA_cen. [Graphical view]
Gene3D	G3DSA:3.90.199.10. Topo_IIA_A/C_ab. 1 hit. G3DSA:1.10.268.10. Topo_IIA_A_a. 1 hit.
KO	K02469.
Pfam	PF03989. DNA_gyraseA_C. 6 hits. PF00521. DNA_topoisoIV. 1 hit. [Graphical view]
SMART	SM00434. TOP4c. 1 hit. [Graphical view]
SUPFAM	SSF56719. Topo_IIA_cen. 1 hit.
TIGRFAMs	TIGR01063. GyrA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GYRA_BUCAI

Accession

Primary (citable) accession number: P57277

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	January 25, 2012
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program