Reviewed, UniProtKB/Swiss-Prot P57276 (RIR1_BUCAI)

Names and origin

Protein names

Recommended name:
    Ribonucleoside-diphosphate reductase subunit alpha
    EC=1.17.4.1
Alternative name(s):
    Ribonucleotide reductase

Gene names

Name:	nrdA
Ordered Locus Names:	BU179

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (Acyrthosiphon pisum symbiotic bacterium) [Complete proteome] [HAMAP]

Taxonomic identifier

118099 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	761 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides By similarity.
Catalytic activity	2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.
Pathway	Genetic information processing; DNA replication.
Subunit structure	Tetramer of two alpha and two beta subunits By similarity.
Sequence similarities	Belongs to the ribonucleoside diphosphate reductase large chain family. Contains 1 ATP-cone domain.

Ontologies

Keywords
Biological process	DNA replication
Ligand	ATP-binding Nucleotide-binding
Molecular function	Oxidoreductase
PTM	Disulfide bond
Technical term	Allosteric enzyme Complete proteome
Gene Ontology (GO)
Biological process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	ribonucleoside-diphosphate reductase complex Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from electronic annotation. Source: InterPro ribonucleoside-diphosphate reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 761	761	Ribonucleoside-diphosphate reductase subunit alpha		PRO_0000187206
Regions
	Domain	5 – 95	91	ATP-cone
Sites
	Active site	225	1	Hydrogen atom transfer By similarity
	Active site	437	1	Proton acceptor By similarity
	Active site	439	1	Proton acceptor By similarity
	Active site	441	1	Proton acceptor By similarity
	Active site	462	1	Hydrogen atom transfer By similarity
	Active site	730	1	Electron transfer By similarity
	Active site	731	1	Electron transfer By similarity
	Site	232	1	Allosteric effector binding By similarity
	Site	262	1	Allosteric effector binding By similarity
	Site	754	1	Interacts with thioredoxin/glutaredoxin By similarity
	Site	759	1	Interacts with thioredoxin/glutaredoxin By similarity
Amino acid modifications
	Disulfide bond	225 ↔ 462		Redox-active By similarity

Sequences

	Sequence		Length	Mass (Da)
	P57276-1 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: 700A2A4AA426AC76	FASTA	761	87,166
	10 20 30 40 50 60 MKKNLFVTKR DGRKEKINLD KIHKVLNWAS EGLDDVSVSQ VELCSRIQFY NNITTINIHE 70 80 90 100 110 120 TIIKAAADLI SQDTPDYQYM AARLAIFHLR KKAYGQFEPP KLYDHVKKMV DLEKYDENLL 130 140 150 160 170 180 KNYSYKEFLQ MNSFIDHWRD MNFSYAAVKQ LEGKYLIQNR VNGKIYESAQ FLYILISACL 190 200 210 220 230 240 FSQYPKNVRM NYIHRFYNAI STFKISLPTP IMSGVRTPTR QFSSCVLIEC GDSLNSINAT 250 260 270 280 290 300 TSSIVKYVSQ RAGIGVNAGQ IRALGSPIRN GEAFHTGCIP FYKHFQSAVK SCSQGGVRGG 310 320 330 340 350 360 AATIFYPIWH LEVESLLVLK NNRGIEENRV RHIDYAIQIN KLMYQRMLSG DQITLFSPSD 370 380 390 400 410 420 VPDLYKAFFS DQKKFKKIYL QYEKNKNIRK KTIKALDLFS LMMQERTSTG RIYVQNVDHC 430 440 450 460 470 480 NLHSAFNPEL SPIRQSNLCL EITLPTKSLN DVHDTDGEIA LCTLSAFNLG KIKSLDDFKE 490 500 510 520 530 540 LSILSVRALD EILDYQNYPV LAAKKSAISR RSLGIGVINF AYYLAKNKVR YSDGSAHNLT 550 560 570 580 590 600 HKTFEAMQYY LLEASCELAK EKGACSLFNH TNYYLGKLPI DTYKKYIDDI CNEPLHLDWN 610 620 630 640 650 660 LLRSKIKKYG LRNSTLSALM PSETSSQISN ATNGIEPPRG FISIKVSKDG ILRQVVPEYK 670 680 690 700 710 720 KLRLQYELLW DIPNNTGYLQ LAGIMQKFID QSISVNTHYD PARFLNNKIP MKQLLYDLLL 730 740 750 760 SYKLGLKTLY YQNTRDGSED DQNITSKSIT EDICESGSCI L

References

[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed: 10993077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tokyo 1998.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000003 Genomic DNA. Translation: BAB12896.1.
RefSeq	NP_240010.1.
3D structure databases
HSSP	HSSP built from PDB template 5R1R based on UniProtKB P00452.
SMR	P57276. Positions 4-737.
ModBase	Search...
Genome annotation databases
GeneID	1109622.
GenomeReviews	Gene locus BU179 in contig BA000003_GR.
KEGG	buc:BU179.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P57276.
OMA	ATLFYPL.
Enzyme and pathway databases
BioCyc	BSP107806:BU179-MON.
Family and domain databases
InterPro	IPR005144. ATP-cone. IPR013346. NrdE_NrdA. IPR013509. Ribncl_Rdtase_lsu_N. IPR000788. Ribncl_red_lg_C. [Graphical view]
PANTHER	PTHR11573. Ribncl_red_lg_C. 1 hit.
Pfam	PF03477. ATP-cone. 1 hit. PF02867. Ribonuc_red_lgC. 1 hit. PF00317. Ribonuc_red_lgN. 1 hit. [Graphical view]
PRINTS	PR01183. RIBORDTASEM1.
TIGRFAMs	TIGR02506. NrdE_NrdA. 1 hit.
PROSITE	PS51161. ATP_CONE. 1 hit. PS00089. RIBORED_LARGE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RIR1_BUCAI

Accession

Primary (citable) accession number: P57276

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	November 3, 2009
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)