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Protein

Ribonucleoside-diphosphate reductase subunit beta

Gene

nrdB

Organism
Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides (By similarity).By similarity

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.PROSITE-ProRule annotation

Cofactori

Fe cationBy similarityNote: Binds 2 iron ions per subunit.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi85 – 851Iron 1PROSITE-ProRule annotation
Metal bindingi116 – 1161Iron 1PROSITE-ProRule annotation
Metal bindingi116 – 1161Iron 2By similarity
Metal bindingi119 – 1191Iron 1PROSITE-ProRule annotation
Active sitei123 – 1231PROSITE-ProRule annotation
Metal bindingi205 – 2051Iron 2By similarity
Metal bindingi239 – 2391Iron 2By similarity
Metal bindingi242 – 2421Iron 2By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-EC

GO - Biological processi

  1. deoxyribonucleoside diphosphate metabolic process Source: InterPro
  2. DNA replication Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BioCyciBAPH107806:GBZJ-177-MONOMER.
UniPathwayiUPA00326.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonucleoside-diphosphate reductase subunit beta (EC:1.17.4.1)
Alternative name(s):
Ribonucleotide reductase small subunit
Gene namesi
Name:nrdB
Ordered Locus Names:BU178
OrganismiBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Taxonomic identifieri107806 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
ProteomesiUP000001806: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 376376Ribonucleoside-diphosphate reductase subunit betaPRO_0000190470Add
BLAST

Interactioni

Subunit structurei

Tetramer of two alpha and two beta subunits.By similarity

Structurei

3D structure databases

ProteinModelPortaliP57275.
SMRiP57275. Positions 2-342.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0208.
HOGENOMiHOG000278087.
KOiK00526.
OMAiCNRRCQQ.
OrthoDBiEOG6J48J7.

Family and domain databases

Gene3Di1.10.620.20. 1 hit.
InterProiIPR009078. Ferritin-like_SF.
IPR012348. RNR-rel.
IPR000358. RNR_small.
[Graphical view]
PANTHERiPTHR23409. PTHR23409. 1 hit.
PfamiPF00268. Ribonuc_red_sm. 1 hit.
[Graphical view]
SUPFAMiSSF47240. SSF47240. 1 hit.
PROSITEiPS00368. RIBORED_SMALL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P57275-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSYTIFSKKK NNQLKEPMFF GQPVNIARYD QQKYKIFEQL IEKQLSFFWR
60 70 80 90 100
PEEIDLSRDR IDFQNLPDNE KHIFISNLKY QTLLDSIQGR SPNIAFLPII
110 120 130 140 150
SIPELETWIE TWSFSETIHS RSYTHIIRNI VNCPSLVFDD IISNKNIYDR
160 170 180 190 200
AQNISIYYDE LINLTSYWHL LGEGIHLING KKIHINLRFL KKRLYLCLIS
210 220 230 240 250
VNVLEAIRFY VSFACSFAFA ERELMEGNAK IIRLIARDEA LHLTGTQHIL
260 270 280 290 300
NLLSNIKNNE NMEDVVLECK EEAINIFISA AQQEKKWASY LFKSGSMLGL
310 320 330 340 350
NKDILCQYIE YITNIRMHAI GFKMPFKKQS NPIPWINDWL TSDNIQIAPQ
360 370
ETEISSYLVG QIDSEVSDNE FKKFEL
Length:376
Mass (Da):44,146
Last modified:December 1, 2000 - v1
Checksum:iBECC1B02F77CBB32
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000003 Genomic DNA. Translation: BAB12895.1.
RefSeqiNP_240009.1. NC_002528.1.

Genome annotation databases

EnsemblBacteriaiBAB12895; BAB12895; BAB12895.
GeneIDi1109621.
KEGGibuc:BU178.
PATRICi21243874. VBIBucAph127364_0189.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000003 Genomic DNA. Translation: BAB12895.1.
RefSeqiNP_240009.1. NC_002528.1.

3D structure databases

ProteinModelPortaliP57275.
SMRiP57275. Positions 2-342.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB12895; BAB12895; BAB12895.
GeneIDi1109621.
KEGGibuc:BU178.
PATRICi21243874. VBIBucAph127364_0189.

Phylogenomic databases

eggNOGiCOG0208.
HOGENOMiHOG000278087.
KOiK00526.
OMAiCNRRCQQ.
OrthoDBiEOG6J48J7.

Enzyme and pathway databases

UniPathwayiUPA00326.
BioCyciBAPH107806:GBZJ-177-MONOMER.

Family and domain databases

Gene3Di1.10.620.20. 1 hit.
InterProiIPR009078. Ferritin-like_SF.
IPR012348. RNR-rel.
IPR000358. RNR_small.
[Graphical view]
PANTHERiPTHR23409. PTHR23409. 1 hit.
PfamiPF00268. Ribonuc_red_sm. 1 hit.
[Graphical view]
SUPFAMiSSF47240. SSF47240. 1 hit.
PROSITEiPS00368. RIBORED_SMALL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
    Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
    Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: APS.

Entry informationi

Entry nameiRIR2_BUCAI
AccessioniPrimary (citable) accession number: P57275
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: January 7, 2015
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Buchnera aphidicola (subsp. Acyrthosiphon pisum)
    Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.