P57275 (RIR2_BUCAI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonucleoside-diphosphate reductase subunit beta
EC=1.17.4.1

Alternative name(s):
Ribonucleotide reductase small subunit

Gene names

Name:	nrdB
Ordered Locus Names:	BU178

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)

Taxonomic identifier

107806 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	376 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides By similarity.
Catalytic activity	2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.
Cofactor	Binds 2 iron ions per subunit By similarity.
Pathway	Genetic information processing; DNA replication.
Subunit structure	Tetramer of two alpha and two beta subunits By similarity.
Sequence similarities	Belongs to the ribonucleoside diphosphate reductase small chain family.

Ontologies

Keywords
Biological process	DNA replication
Ligand	Iron Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW deoxyribonucleoside diphosphate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	ribonucleoside-diphosphate reductase activity Inferred from electronic annotation. Source: EC transition metal ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 376	376	Ribonucleoside-diphosphate reductase subunit beta		PRO_0000190470
Sites
	Active site	123	1	By similarity
	Metal binding	85	1	Iron 1 By similarity
	Metal binding	116	1	Iron 1 By similarity
	Metal binding	116	1	Iron 2 By similarity
	Metal binding	119	1	Iron 1 By similarity
	Metal binding	205	1	Iron 2 By similarity
	Metal binding	239	1	Iron 2 By similarity
	Metal binding	242	1	Iron 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	P57275 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: BECC1B02F77CBB32	FASTA	376	44,146
	10 20 30 40 50 60 MSYTIFSKKK NNQLKEPMFF GQPVNIARYD QQKYKIFEQL IEKQLSFFWR PEEIDLSRDR 70 80 90 100 110 120 IDFQNLPDNE KHIFISNLKY QTLLDSIQGR SPNIAFLPII SIPELETWIE TWSFSETIHS 130 140 150 160 170 180 RSYTHIIRNI VNCPSLVFDD IISNKNIYDR AQNISIYYDE LINLTSYWHL LGEGIHLING 190 200 210 220 230 240 KKIHINLRFL KKRLYLCLIS VNVLEAIRFY VSFACSFAFA ERELMEGNAK IIRLIARDEA 250 260 270 280 290 300 LHLTGTQHIL NLLSNIKNNE NMEDVVLECK EEAINIFISA AQQEKKWASY LFKSGSMLGL 310 320 330 340 350 360 NKDILCQYIE YITNIRMHAI GFKMPFKKQS NPIPWINDWL TSDNIQIAPQ ETEISSYLVG 370 QIDSEVSDNE FKKFEL

References

[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed: 10993077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: APS.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000003 Genomic DNA. Translation: BAB12895.1.
RefSeq	NP_240009.1. NC_002528.1.
3D structure databases
ProteinModelPortal	P57275.
SMR	P57275. Positions 2-342.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBUCT00000003308; EBBUCP00000003084; EBBUCG00000003308.
GeneID	1109621.
GenomeReviews	Gene locus BU178 in contig BA000003_GR.
KEGG	buc:BU178.
PATRIC	21243874. VBIBucAph127364_0189.
Phylogenomic databases
GeneTree	EBGT00050000007938.
HOGENOM	HBG296569.
OMA	QHMLNLM.
PhylomeDB	P57275.
ProtClustDB	PRK09101.
Enzyme and pathway databases
BioCyc	BSP107806:BU178-MONOMER.
Family and domain databases
InterPro	IPR009078. Ferritin/RR-like. IPR012348. Ribncl_red-rel. IPR000358. Ribonucl_redctse. [Graphical view]
Gene3D	G3DSA:1.10.620.20. Ribncl_red_rel. 1 hit.
KO	K00526.
PANTHER	PTHR23409. Ribonucl_redctse. 1 hit.
Pfam	PF00268. Ribonuc_red_sm. 1 hit. [Graphical view]
SUPFAM	SSF47240. Ferritin/RR_like. 1 hit.
PROSITE	PS00368. RIBORED_SMALL. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RIR2_BUCAI

Accession

Primary (citable) accession number: P57275

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	January 25, 2012
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program