P57271 (NADE_BUCAI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NH(3)-dependent NAD(+) synthetase
EC=6.3.1.5

Gene names

Name:	nadE
Ordered Locus Names:	BU174

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)

Taxonomic identifier

107806 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	268 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193
Sequence similarities	Belongs to the NAD synthetase family.

Ontologies

Keywords
Ligand	ATP-binding NAD Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 268	268	NH(3)-dependent NAD(+) synthetase HAMAP MF_00193		PRO_0000152160
Regions
	Nucleotide binding	46 – 53	8	ATP By similarity
Sites
	Active site	48	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P57271 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: 55ED78A6EDF98112	FASTA	268	30,430
	10 20 30 40 50 60 MTLQKKIIEL LGVKPAIIPE IEIKNCVDFL KKYLVNHVHI KSLIVGVSGG QDSTLTAKLC 70 80 90 100 110 120 QMTAETLRKE KNDITYQFIA LRLPYGIQYD EKDCQDAIRF IQPDQIFNVN IKKAVLSSEK 130 140 150 160 170 180 SLKKSGVIIS DYVRGNEKAR ERMKVQYSIA AMKQGLVVGT GHAAENITGF FTKYGDSGTD 190 200 210 220 230 240 INPIAKLNKR QIRLLLKNLN CPKHLYLKKP MADLEDEHPQ QDDESVLGVT YDAIDSYLEQ 250 260 KKIDIRSQKI IEALYLNTLH KRNLPITQ

References

[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed: 10993077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: APS.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000003 Genomic DNA. Translation: BAB12891.1.
RefSeq	NP_240005.1. NC_002528.1.
3D structure databases
ProteinModelPortal	P57271.
SMR	P57271. Positions 2-267.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBUCT00000002883; EBBUCP00000002659; EBBUCG00000002883.
GeneID	1109617.
GenomeReviews	Gene locus BU174 in contig BA000003_GR.
KEGG	buc:BU174.
PATRIC	21243866. VBIBucAph127364_0185.
Phylogenomic databases
GeneTree	EBGT00050000008132.
HOGENOM	HBG351567.
OMA	GLNKRRV.
PhylomeDB	P57271.
ProtClustDB	PRK00768.
Enzyme and pathway databases
BioCyc	BSP107806:BU174-MONOMER.
Family and domain databases
HAMAP	MF_00193. NadE. [Tree]
InterPro	IPR022310. NAD/GMP_synthase. IPR003694. NAD_synthase. IPR022926. NH(3)-dep_NAD(+)_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01916.
Pfam	PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00552. NadE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NADE_BUCAI

Accession

Primary (citable) accession number: P57271

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	January 25, 2012
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program