P57267 (ISPE_BUCAI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
Short name=CMK
EC=2.7.1.148

Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Gene names

Name:	ispE
Ordered Locus Names:	BU170

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)

Taxonomic identifier

107806 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	286 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity. HAMAP MF_00061
Catalytic activity	ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00061
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP MF_00061
Subunit structure	Homodimer By similarity. HAMAP MF_00061
Sequence similarities	Belongs to the GHMP kinase family. IspE subfamily.
Sequence caution	The sequence BAB12887.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 286	286	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP MF_00061		PRO_0000189197
Regions
	Nucleotide binding	100 – 110	11	ATP Potential
Sites
	Active site	10	1	By similarity
	Active site	142	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P57267 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: E9BDB6766131AF60	FASTA	286	32,040
	10 20 30 40 50 60 MIYTCLSPAK INLFLYVTGR RKDGYHNIQT LFQFLDYGDQ FKIIANKTGN IELFTEKKIF 70 80 90 100 110 120 MNVQNSIIIA AKLLKKTALL QGKLQNSSYG AKIFLKKNIP MGSGLGGGSS NAATTLVVLN 130 140 150 160 170 180 KLWNTQYTLK ELSLLGLRIG ADVPGFVMGN TAVIEGIGDI LYPIVQKEKW YLVVYPCINI 190 200 210 220 230 240 STRYMFSSPF LMSNTAKKSL QVLLKTPFKN DFENIAKKQF VPIKKLIRML SSYAPSRMTG 250 260 270 280 TGSCVFSEFD NKKSAQKIFS VLPKNVQGFI AKSVNISPLH KTLYKR

References

[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed: 10993077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: APS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000003 Genomic DNA. Translation: BAB12887.1. Different initiation.
RefSeq	NP_240001.1. NC_002528.1.
3D structure databases
ProteinModelPortal	P57267.
SMR	P57267. Positions 1-283.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBUCT00000002839; EBBUCP00000002615; EBBUCG00000002839.
GeneID	1109613.
GenomeReviews	Gene locus BU170 in contig BA000003_GR.
KEGG	buc:BU170.
PATRIC	21243858. VBIBucAph127364_0181.
Phylogenomic databases
GeneTree	EBGT00050000008174.
HOGENOM	HBG734593.
OMA	LNLFLHV.
PhylomeDB	P57267.
ProtClustDB	PRK00343.
Enzyme and pathway databases
BioCyc	BSP107806:BU170-MONOMER.
Family and domain databases
HAMAP	MF_00061. IspE. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR004424. IspE. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00919.
PANTHER	PTHR20861:SF2. IspE. 1 hit.
Pfam	PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF010376. IspE. 1 hit.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00154. IspE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPE_BUCAI

Accession

Primary (citable) accession number: P57267

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	January 25, 2012
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program