Reviewed, UniProtKB/Swiss-Prot P57267 (ISPE_BUCAI)

Names and origin

Protein names

Recommended name:
    4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
      Short name=CMK
    EC=2.7.1.148
Alternative name(s):
    4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Gene names

Name:	ispE
Ordered Locus Names:	BU170

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (Acyrthosiphon pisum symbiotic bacterium) [Complete proteome] [HAMAP]

Taxonomic identifier

118099 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	286 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity. HAMAP MF_00061
Catalytic activity	ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00061
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP MF_00061
Subunit structure	Homodimer By similarity. HAMAP MF_00061
Sequence similarities	Belongs to the GHMP kinase family. IspE subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phosphorylation Inferred from electronic annotation. Source: InterPro terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Inferred from electronic annotation. Source: HAMAP ATP binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 286	286	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP MF_00061		PRO_0000189197
Regions
	Nucleotide binding	100 – 110	11	ATP Potential
Sites
	Active site	10	1	By similarity
	Active site	142	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P57267-1 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: E9BDB6766131AF60	FASTA	286	32,040
	10 20 30 40 50 60 MIYTCLSPAK INLFLYVTGR RKDGYHNIQT LFQFLDYGDQ FKIIANKTGN IELFTEKKIF 70 80 90 100 110 120 MNVQNSIIIA AKLLKKTALL QGKLQNSSYG AKIFLKKNIP MGSGLGGGSS NAATTLVVLN 130 140 150 160 170 180 KLWNTQYTLK ELSLLGLRIG ADVPGFVMGN TAVIEGIGDI LYPIVQKEKW YLVVYPCINI 190 200 210 220 230 240 STRYMFSSPF LMSNTAKKSL QVLLKTPFKN DFENIAKKQF VPIKKLIRML SSYAPSRMTG 250 260 270 280 TGSCVFSEFD NKKSAQKIFS VLPKNVQGFI AKSVNISPLH KTLYKR

References

[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed: 10993077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tokyo 1998.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000003 Genomic DNA. Translation: BAB12887.1. Different initiation.
RefSeq	NP_240001.1.
3D structure databases
SMR	P57267. Positions 1-283.
ModBase	Search...
Genome annotation databases
GeneID	1109613.
GenomeReviews	Gene locus BU170 in contig BA000003_GR.
KEGG	buc:BU170.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG734593.
PhylomeDB	P57267.
Enzyme and pathway databases
BioCyc	BSP107806:BU170-MONOMER.
Family and domain databases
HAMAP	MF_00061. IspE. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR004424. IspE. IPR020568. Ribosomal_S5_D2-typ_fold. [Graphical view]
PANTHER	PTHR20861:SF2. IspE. 1 hit.
Pfam	PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF010376. IspE. 1 hit.
TIGRFAMs	TIGR00154. ispE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPE_BUCAI

Accession

Primary (citable) accession number: P57267

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	February 9, 2010
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)