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Protein

Dihydrofolate synthase/folylpolyglutamate synthase

Gene

folC

Organism
Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Functions in two distinct reactions of the de novo folate biosynthetic pathway. Catalyzes the addition of a glutamate residue to dihydropteroate (7,8-dihydropteroate or H2Pte) to form dihydrofolate (7,8-dihydrofolate monoglutamate or H2Pte-Glu). Also catalyzes successive additions of L-glutamate to tetrahydrofolate or 10-formyltetrahydrofolate or 5,10-methylenetetrahydrofolate, leading to folylpolyglutamate derivatives.By similarity

Catalytic activityi

ATP + 7,8-dihydropteroate + L-glutamate = ADP + phosphate + 7,8-dihydropteroylglutamate.By similarity
ATP + tetrahydropteroyl-(gamma-Glu)(n) + L-glutamate = ADP + phosphate + tetrahydropteroyl-(gamma-Glu)(n+1).By similarity
ATP + 10-formyl-tetrahydropteroyl-(gamma-Glu)(n) + L-glutamate = ADP + phosphate + 10-formyl-tetrahydropteroyl-(gamma-Glu)(n+1).By similarity
ATP + 5,10-methylene-tetrahydropteroyl-(gamma-Glu)(n) + L-glutamate = ADP + phosphate + 5,10-methylene-tetrahydropteroyl-(gamma-Glu)(n+1).By similarity

Cofactori

Mg2+By similarityNote: Binds 2 Mg2+ ions per subunit.By similarity

Pathwayi: tetrahydrofolate biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes 7,8-dihydrofolate from 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate and 4-aminobenzoate.By similarity
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Dihydrofolate synthase/folylpolyglutamate synthase (folC)
This subpathway is part of the pathway tetrahydrofolate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 7,8-dihydrofolate from 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate and 4-aminobenzoate, the pathway tetrahydrofolate biosynthesis and in Cofactor biosynthesis.

Pathwayi: tetrahydrofolylpolyglutamate biosynthesis

This protein is involved in the pathway tetrahydrofolylpolyglutamate biosynthesis, which is part of Cofactor biosynthesis.By similarity
View all proteins of this organism that are known to be involved in the pathway tetrahydrofolylpolyglutamate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi77Magnesium 1By similarity1
Metal bindingi167Magnesium 2By similarity1
Binding sitei283ATPBy similarity1
Binding sitei296ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi53 – 56ATPBy similarity4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processFolate biosynthesis, One-carbon metabolism
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00077; UER00157.
UPA00850.

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydrofolate synthase/folylpolyglutamate synthase (EC:6.3.2.12, EC:6.3.2.17)
Short name:
DHFS / FPGS
Alternative name(s):
Folylpoly-gamma-glutamate synthetase-dihydrofolate synthetase
Folylpolyglutamate synthetase
Tetrahydrofolylpolyglutamate synthase
Gene namesi
Name:folC
Ordered Locus Names:BU167
OrganismiBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Taxonomic identifieri107806 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesErwiniaceaeBuchnera
Proteomesi
  • UP000001806 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001683001 – 411Dihydrofolate synthase/folylpolyglutamate synthaseAdd BLAST411

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi107806.BU167.

Structurei

3D structure databases

ProteinModelPortaliP57265.
SMRiP57265.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni116 – 1197,8-dihydropteroate bindingBy similarity4
Regioni147 – 1497,8-dihydropteroate bindingBy similarity3

Sequence similaritiesi

Belongs to the folylpolyglutamate synthase family.Curated

Phylogenomic databases

eggNOGiENOG4105DPM. Bacteria.
COG0285. LUCA.
HOGENOMiHOG000019982.
KOiK11754.
OMAiLTYFEMG.

Family and domain databases

Gene3Di3.40.1190.10. 1 hit.
3.90.190.20. 1 hit.
InterProiView protein in InterPro
IPR001645. Folylpolyglutamate_synth.
IPR018109. Folylpolyglutamate_synth_CS.
IPR004101. Mur_ligase_C.
IPR013221. Mur_ligase_cen.
PANTHERiPTHR11136. PTHR11136. 1 hit.
PfamiView protein in Pfam
PF08245. Mur_ligase_M. 1 hit.
PIRSFiPIRSF001563. Folylpolyglu_synth. 1 hit.
SUPFAMiSSF53244. SSF53244. 1 hit.
SSF53623. SSF53623. 1 hit.
TIGRFAMsiTIGR01499. folC. 1 hit.
PROSITEiView protein in PROSITE
PS01011. FOLYLPOLYGLU_SYNT_1. 1 hit.
PS01012. FOLYLPOLYGLU_SYNT_2. 1 hit.

Sequencei

Sequence statusi: Complete.

P57265-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MINKNYSLSL WLKYLEQLDK KRIYNLTELK FLAKKLGLLK SESFIFTVAG
60 70 80 90 100
TNGKGTTCAV LERLLLDSGY QVGLYTSPHL INFVERVRIN GFVLHEEEHI
110 120 130 140 150
DSFQNVELVR NGVLLTYFEF ITLAALILFK RYSLDCIILK VGLGGRLDAT
160 170 180 190 200
NIIDSDISII TNIGIDHTSI LGRDRISIAR EKCGVFRKNK ISVIGETDIP
210 220 230 240 250
CSMYQIAKEK KTILKKIDID WSWEKKRNYW NFFHSTIQLY NLPETQVPLS
260 270 280 290 300
SAATALSTLY YSRFKIKEKI IRKSISNVQL PGRFQVISTF PYIIVDVAHN
310 320 330 340 350
PNAAFYLSQK IDEINITGKI YAVVGILKDK DILGIIDPLA NKIHHWFTAP
360 370 380 390 400
LKTIRTATKH ELKKFFPIHN TSILKSIEIA YKKALILVKK EDAIIIFGSF
410
LTVSEFLSLK I
Length:411
Mass (Da):46,970
Last modified:December 1, 2000 - v1
Checksum:i5DDC2DC66539935A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000003 Genomic DNA. Translation: BAB12885.1.
RefSeqiNP_239999.1. NC_002528.1.
WP_010895983.1. NC_002528.1.

Genome annotation databases

EnsemblBacteriaiBAB12885; BAB12885; BAB12885.
GeneIDi1109611.
KEGGibuc:BU167.
PATRICi21243850. VBIBucAph127364_0177.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000003 Genomic DNA. Translation: BAB12885.1.
RefSeqiNP_239999.1. NC_002528.1.
WP_010895983.1. NC_002528.1.

3D structure databases

ProteinModelPortaliP57265.
SMRiP57265.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi107806.BU167.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB12885; BAB12885; BAB12885.
GeneIDi1109611.
KEGGibuc:BU167.
PATRICi21243850. VBIBucAph127364_0177.

Phylogenomic databases

eggNOGiENOG4105DPM. Bacteria.
COG0285. LUCA.
HOGENOMiHOG000019982.
KOiK11754.
OMAiLTYFEMG.

Enzyme and pathway databases

UniPathwayiUPA00077; UER00157.
UPA00850.

Family and domain databases

Gene3Di3.40.1190.10. 1 hit.
3.90.190.20. 1 hit.
InterProiView protein in InterPro
IPR001645. Folylpolyglutamate_synth.
IPR018109. Folylpolyglutamate_synth_CS.
IPR004101. Mur_ligase_C.
IPR013221. Mur_ligase_cen.
PANTHERiPTHR11136. PTHR11136. 1 hit.
PfamiView protein in Pfam
PF08245. Mur_ligase_M. 1 hit.
PIRSFiPIRSF001563. Folylpolyglu_synth. 1 hit.
SUPFAMiSSF53244. SSF53244. 1 hit.
SSF53623. SSF53623. 1 hit.
TIGRFAMsiTIGR01499. folC. 1 hit.
PROSITEiView protein in PROSITE
PS01011. FOLYLPOLYGLU_SYNT_1. 1 hit.
PS01012. FOLYLPOLYGLU_SYNT_2. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiFOLC_BUCAI
AccessioniPrimary (citable) accession number: P57265
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: March 15, 2017
This is version 97 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Buchnera aphidicola (subsp. Acyrthosiphon pisum)
    Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.