P57264 (NUON_BUCAI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADH-quinone oxidoreductase subunit N
EC=1.6.99.5

Alternative name(s):
NADH dehydrogenase I subunit N
NDH-1 subunit N

Gene names

Name:	nuoN
Ordered Locus Names:	BU166

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)

Taxonomic identifier

107806 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	486 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient By similarity. HAMAP MF_00445
Catalytic activity	NADH + quinone = NAD+ + quinol. HAMAP MF_00445
Subunit structure	NDH-1 is composed of 13 different subunits. Subunits NuoA, H, J, K, L, M, N constitute the membrane sector of the complex By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein HAMAP MF_00445.
Sequence similarities	Belongs to the complex I subunit 2 family.
Sequence caution	The sequence BAB12884.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	NAD Ubiquinone
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	ATP synthesis coupled electron transport Inferred from electronic annotation. Source: InterPro transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 486	486	NADH-quinone oxidoreductase subunit N HAMAP MF_00445		PRO_0000117690
Regions
	Transmembrane	8 – 28	21	Helical; Potential
	Transmembrane	36 – 56	21	Helical; Potential
	Transmembrane	74 – 94	21	Helical; Potential
	Transmembrane	104 – 124	21	Helical; Potential
	Transmembrane	125 – 145	21	Helical; Potential
	Transmembrane	160 – 180	21	Helical; Potential
	Transmembrane	201 – 221	21	Helical; Potential
	Transmembrane	239 – 259	21	Helical; Potential
	Transmembrane	269 – 289	21	Helical; Potential
	Transmembrane	298 – 318	21	Helical; Potential
	Transmembrane	329 – 349	21	Helical; Potential
	Transmembrane	376 – 396	21	Helical; Potential
	Transmembrane	410 – 432	23	Helical; Potential
	Transmembrane	459 – 479	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	P57264 [UniParc]. Last modified September 19, 2006. Version 2. Checksum: 0BE35CC86C83D438	FASTA	486	54,481
	10 20 30 40 50 60 MIINLQQLTA LLPLLIIMLT VITVILSISY NRNHFFVAVF SILGLIFALC SLYFLIEIIP 70 80 90 100 110 120 INVSILFHFN SNAILYIGMI LISSICTCIF SYPWLLKYPF NKEEFYLLVI ISTLGAISLT 130 140 150 160 170 180 ISHHMASFFI NIELISLPMF GLIAYSRYQK YSLESSLKYI ILSGVSSSFL LFGIAWVYSI 190 200 210 220 230 240 SGGLDFLSIH KSFNFASEKE ILVVLFGISM ILLSLFFKLS IVPFHLWTPD IYQGSPTSVL 250 260 270 280 290 300 SFFSTAGKIS VFSVLLNFLS YFSNSDNKVI YFILSLIIIL SILVGNLMAL FQKDIKRFLG 310 320 330 340 350 360 YTSISQIGYL LIVLLVSHKN YSFSLEASAI YLCGYLFSNI ACLGIVNLIS TSHINNNASS 370 380 390 400 410 420 INSYRGLFWS HPLLSSVLTL VLISSAGIPM TLGFIGKFYI LSIVMIEHLW LIGFAFLIGS 430 440 450 460 470 480 LLGLYCYLRI ILNLYLHPSK LFKRDLNIPL NWVYTPSGIV ICISGIILLA LGIYPNPLIG LIKCTI

References

[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed: 10993077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: APS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000003 Genomic DNA. Translation: BAB12884.1. Different initiation.
RefSeq	NP_239998.1. NC_002528.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBUCT00000003196; EBBUCP00000002972; EBBUCG00000003196.
GeneID	1109610.
GenomeReviews	Gene locus BU166 in contig BA000003_GR.
KEGG	buc:BU166.
PATRIC	21243848. VBIBucAph127364_0176.
Phylogenomic databases
GeneTree	EBGT00050000007699.
HOGENOM	HBG747830.
OMA	ILSISYN.
PhylomeDB	P57264.
ProtClustDB	CLSK315621.
Enzyme and pathway databases
BioCyc	BSP107806:BU166-MONOMER.
Family and domain databases
HAMAP	MF_00445. NDH1_NuoN_1. [Tree]
InterPro	IPR010096. NADH-Q_OxRdtase_suN/2. IPR001750. NADH_UbQ/plastoQ_OxRdtase. [Graphical view]
KO	K00343.
Pfam	PF00361. Oxidored_q1. 1 hit. [Graphical view]
TIGRFAMs	TIGR01770. NDH_I_N. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NUON_BUCAI

Accession

Primary (citable) accession number: P57264

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	September 19, 2006
Last modified:	January 25, 2012
This is version 62 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program