P57263 (NUOM_BUCAI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADH-quinone oxidoreductase subunit M
EC=1.6.99.5

Alternative name(s):
NADH dehydrogenase I subunit M
NDH-1 subunit M

Gene names

Name:	nuoM
Ordered Locus Names:	BU165

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)

Taxonomic identifier

107806 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	505 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient By similarity.
Catalytic activity	NADH + quinone = NAD+ + quinol.
Subunit structure	Composed of 13 different subunits. Subunits NuoA, H, J, K, L, M, N constitute the membrane sector of the complex By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein Potential.
Sequence similarities	Belongs to the complex I subunit 4 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	NAD
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	ATP synthesis coupled electron transport Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 505	505	NADH-quinone oxidoreductase subunit M		PRO_0000118041
Regions
	Transmembrane	30 – 50	21	Helical; Potential
	Transmembrane	86 – 106	21	Helical; Potential
	Transmembrane	114 – 134	21	Helical; Potential
	Transmembrane	136 – 156	21	Helical; Potential
	Transmembrane	173 – 193	21	Helical; Potential
	Transmembrane	221 – 241	21	Helical; Potential
	Transmembrane	251 – 271	21	Helical; Potential
	Transmembrane	285 – 305	21	Helical; Potential
	Transmembrane	313 – 333	21	Helical; Potential
	Transmembrane	373 – 393	21	Helical; Potential
	Transmembrane	409 – 429	21	Helical; Potential
	Transmembrane	462 – 482	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	P57263 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: 69C60A2B9ADF3ED9	FASTA	505	57,826
	10 20 30 40 50 60 MLLSLLIIIP FLSSFFSFFS PRLHNNFPRW IALSGIIATL LVVIQIFFQE NYHIFQIRHY 70 80 90 100 110 120 PNWNCQLIVP WISRFGIEFN IALDGLSIIM LIFSSFLSII AIICSWNEIK KNEGFFYFNF 130 140 150 160 170 180 MLVFTGIIGV FISCDLFLFF CFWEIMLIPM YFLIALWSDK TEKKKNFLAA NKFFLYSQTS 190 200 210 220 230 240 GLILLSSILL LVFSHYYSTN ILTFNYNLLI NKPINIYVEY IVMIGFFLSF AIKMPIVPFH 250 260 270 280 290 300 GWLPDIHSRS LSCGSVEIIG VLLKTAPYAL LRYNLVLFPD STKSFSLIAV FWGIISIFYG 310 320 330 340 350 360 AWIAFSQTNI KRLIAYSSVS HMGLILIGIY SNNERALQGV VIQMLSNSLT VAALCILSGQ 370 380 390 400 410 420 IYKRFKTQDM SKMGGLWSCI YWIPGFSLFF SLANLGVPGT GNFIGEFLIL SGVFEVFPLV 430 440 450 460 470 480 SILATIGIVF SSIYSLNVIQ KIFYGPCKQN IKVFFINKQE VWTIIALVFT LVFLGLNPQK 490 500 IIDVSYNSIH NIQKEFNNSI LKIRS

References

[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed: 10993077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: APS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000003 Genomic DNA. Translation: BAB12883.1.
RefSeq	NP_239997.1. NC_002528.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBUCT00000002840; EBBUCP00000002616; EBBUCG00000002840.
GeneID	1109609.
GenomeReviews	Gene locus BU165 in contig BA000003_GR.
KEGG	buc:BU165.
PATRIC	21243846. VBIBucAph127364_0175.
Phylogenomic databases
GeneTree	EBGT00050000007699.
HOGENOM	HBG643593.
OMA	PLAVICI.
PhylomeDB	P57263.
ProtClustDB	CLSK315615.
Enzyme and pathway databases
BioCyc	BSP107806:BU165-MONOMER.
Family and domain databases
InterPro	IPR010227. NADH_Q_OxRdtase_chainM/4. IPR001750. NADH_UbQ/plastoQ_OxRdtase. IPR003918. NADH_UbQ_OxRdtase. [Graphical view]
KO	K00342.
Pfam	PF00361. Oxidored_q1. 1 hit. [Graphical view]
PRINTS	PR01437. NUOXDRDTASE4.
TIGRFAMs	TIGR01972. NDH_I_M. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NUOM_BUCAI

Accession

Primary (citable) accession number: P57263

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	January 25, 2012
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program