P57262 (NUOL_BUCAI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADH-quinone oxidoreductase subunit L
EC=1.6.99.5

Alternative name(s):
NADH dehydrogenase I subunit L
NDH-1 subunit L

Gene names

Name:	nuoL
Ordered Locus Names:	BU164

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium) [Reference proteome] [HAMAP]

Taxonomic identifier

107806 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera › Buchnera aphidicola › Buchnera aphidicola (Acyrthosiphon pisum)

Protein attributes

Sequence length	614 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient By similarity.
Catalytic activity	NADH + quinone = NAD+ + quinol.
Subunit structure	Composed of 13 different subunits. Subunits NuoA, H, J, K, L, M, N constitute the membrane sector of the complex By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein Potential.
Sequence similarities	Belongs to the complex I subunit 5 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	NAD
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ATP synthesis coupled electron transport Inferred from electronic annotation. Source: InterPro
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 614	614	NADH-quinone oxidoreductase subunit L		PRO_0000118214
Regions
	Transmembrane	1 – 21	21	Helical; Potential
	Transmembrane	33 – 53	21	Helical; Potential
	Transmembrane	79 – 99	21	Helical; Potential
	Transmembrane	136 – 156	21	Helical; Potential
	Transmembrane	168 – 188	21	Helical; Potential
	Transmembrane	207 – 227	21	Helical; Potential
	Transmembrane	247 – 267	21	Helical; Potential
	Transmembrane	271 – 291	21	Helical; Potential
	Transmembrane	327 – 347	21	Helical; Potential
	Transmembrane	372 – 392	21	Helical; Potential
	Transmembrane	410 – 430	21	Helical; Potential
	Transmembrane	455 – 475	21	Helical; Potential
	Transmembrane	492 – 512	21	Helical; Potential
	Transmembrane	533 – 553	21	Helical; Potential
	Transmembrane	593 – 613	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	P57262 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: 18CCC2DFC4FE27E0	FASTA	614	70,882
	10 20 30 40 50 60 MSIIFFIILF PLIGFLFLST IQDFIFKRYT LNIGIFSIFI SFFITCFYGV SILKNNNQVF 70 80 90 100 110 120 TQILWKWLSI NEFKIDFGFF LDGLSLSMLF VITGVGLLIH IFSSWYMRYK EGQSRFFAYT 130 140 150 160 170 180 NLFIASMSVL VLADNFLFMY LGWEGVSVCS YLLIGFYYTE LKNNLCAFKA FILTRVSDVF 190 200 210 220 230 240 LMIGMFLIYR EFNSFNFQEI KFLSSFLNVE NFYYLDYITL FLLLGVIGKS AQLPLQTWLS 250 260 270 280 290 300 DAMVGPTPVS ALIHAATMVT AGVYLIARTH FLFLLTPGIL YLVGLIGTLT ILVSSISALV 310 320 330 340 350 360 QKDIKRILAY STMSQIGYMF LALGVKAWSA AITHLIMHAI FKALLFLSAG SLIKSCKNEK 370 380 390 400 410 420 NIFKMGGLRK QLPFLYISFI VGGASLVSFP LITAGFYSKG NILFSVLKSG CIDFFIIGLF 430 440 450 460 470 480 CSFLTAIYTF RMIFVIFHGK NIHTADSSTN LQHNIPLFVL LLLSTVFGSY ISPPLSDVFP 490 500 510 520 530 540 LSYTPIDHKF AFEIICSILS LSGIYLSYYI WIKNLYVLDK IFQFKFMRYL YYFFLKGWGF 550 560 570 580 590 600 NWFYKISFVY FYLYISKRLS ADPLNKIINY FLKVTQIFNF YLLKTSNGYV RWYVASMILG 610 INFIFLLMLF FYFN

References

[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: APS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000003 Genomic DNA. Translation: BAB12882.1.
RefSeq	NP_239996.1. NC_002528.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAB12882; BAB12882; BAB12882.
GeneID	1109608.
KEGG	buc:BU164.
PATRIC	21243844. VBIBucAph127364_0174.
Phylogenomic databases
eggNOG	COG1009.
HOGENOM	HOG000100570.
KO	K00341.
OMA	LIARTHF.
ProtClustDB	CLSK315611.
Family and domain databases
InterPro	IPR001750. NADH_UbQ/plastoQ_OxRdtase. IPR001516. NADH_UbQ_OxRdtase_chain5/L_N. IPR003945. NADHpl_OxRdtase_5. IPR018393. NADHpl_OxRdtase_5_subgr. [Graphical view]
Pfam	PF00361. Oxidored_q1. 1 hit. PF00662. Oxidored_q1_N. 1 hit. [Graphical view]
PRINTS	PR01435. NPOXDRDTASE5.
TIGRFAMs	TIGR01974. NDH_I_L. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NUOL_BUCAI

Accession

Primary (citable) accession number: P57262

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	May 1, 2013
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program