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P57262 (NUOL_BUCAI) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NADH-quinone oxidoreductase subunit L

EC=1.6.99.5
Alternative name(s):
NADH dehydrogenase I subunit L
NDH-1 subunit L
Gene names
Name:nuoL
Ordered Locus Names:BU164
OrganismBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium) [Reference proteome] [HAMAP]
Taxonomic identifier107806 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera

Protein attributes

Sequence length614 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient By similarity.

Catalytic activity

NADH + quinone = NAD+ + quinol.

Subunit structure

Composed of 13 different subunits. Subunits NuoA, H, J, K, L, M, N constitute the membrane sector of the complex By similarity.

Subcellular location

Cell membrane; Multi-pass membrane protein Potential.

Sequence similarities

Belongs to the complex I subunit 5 family.

Ontologies

Keywords
   Cellular componentCell membrane
Membrane
   DomainTransmembrane
Transmembrane helix
   LigandNAD
   Molecular functionOxidoreductase
   PTMQuinone
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processATP synthesis coupled electron transport

Inferred from electronic annotation. Source: InterPro

   Cellular_componentintegral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionNADH dehydrogenase (ubiquinone) activity

Inferred from electronic annotation. Source: InterPro

quinone binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 614614NADH-quinone oxidoreductase subunit L
PRO_0000118214

Regions

Transmembrane1 – 2121Helical; Potential
Transmembrane33 – 5321Helical; Potential
Transmembrane79 – 9921Helical; Potential
Transmembrane136 – 15621Helical; Potential
Transmembrane168 – 18821Helical; Potential
Transmembrane207 – 22721Helical; Potential
Transmembrane247 – 26721Helical; Potential
Transmembrane271 – 29121Helical; Potential
Transmembrane327 – 34721Helical; Potential
Transmembrane372 – 39221Helical; Potential
Transmembrane410 – 43021Helical; Potential
Transmembrane455 – 47521Helical; Potential
Transmembrane492 – 51221Helical; Potential
Transmembrane533 – 55321Helical; Potential
Transmembrane593 – 61321Helical; Potential

Sequences

Sequence LengthMass (Da)Tools
P57262 [UniParc].

Last modified December 1, 2000. Version 1.
Checksum: 18CCC2DFC4FE27E0

FASTA61470,882
        10         20         30         40         50         60 
MSIIFFIILF PLIGFLFLST IQDFIFKRYT LNIGIFSIFI SFFITCFYGV SILKNNNQVF 

        70         80         90        100        110        120 
TQILWKWLSI NEFKIDFGFF LDGLSLSMLF VITGVGLLIH IFSSWYMRYK EGQSRFFAYT 

       130        140        150        160        170        180 
NLFIASMSVL VLADNFLFMY LGWEGVSVCS YLLIGFYYTE LKNNLCAFKA FILTRVSDVF 

       190        200        210        220        230        240 
LMIGMFLIYR EFNSFNFQEI KFLSSFLNVE NFYYLDYITL FLLLGVIGKS AQLPLQTWLS 

       250        260        270        280        290        300 
DAMVGPTPVS ALIHAATMVT AGVYLIARTH FLFLLTPGIL YLVGLIGTLT ILVSSISALV 

       310        320        330        340        350        360 
QKDIKRILAY STMSQIGYMF LALGVKAWSA AITHLIMHAI FKALLFLSAG SLIKSCKNEK 

       370        380        390        400        410        420 
NIFKMGGLRK QLPFLYISFI VGGASLVSFP LITAGFYSKG NILFSVLKSG CIDFFIIGLF 

       430        440        450        460        470        480 
CSFLTAIYTF RMIFVIFHGK NIHTADSSTN LQHNIPLFVL LLLSTVFGSY ISPPLSDVFP 

       490        500        510        520        530        540 
LSYTPIDHKF AFEIICSILS LSGIYLSYYI WIKNLYVLDK IFQFKFMRYL YYFFLKGWGF 

       550        560        570        580        590        600 
NWFYKISFVY FYLYISKRLS ADPLNKIINY FLKVTQIFNF YLLKTSNGYV RWYVASMILG 

       610 
INFIFLLMLF FYFN 

« Hide

References

[1]"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: APS.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000003 Genomic DNA. Translation: BAB12882.1.
RefSeqNP_239996.1. NC_002528.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAB12882; BAB12882; BAB12882.
GeneID1109608.
KEGGbuc:BU164.
PATRIC21243844. VBIBucAph127364_0174.

Phylogenomic databases

eggNOGCOG1009.
HOGENOMHOG000100570.
KOK00341.
OMARFFSYMN.
OrthoDBEOG6FZ48R.
ProtClustDBCLSK315611.

Enzyme and pathway databases

BioCycBAPH107806:GBZJ-164-MONOMER.

Family and domain databases

InterProIPR001750. NADH_UbQ/plastoQ_OxRdtase.
IPR001516. NADH_UbQ_OxRdtase_chain5/L_N.
IPR003945. NADHpl_OxRdtase_5.
IPR018393. NADHpl_OxRdtase_5_subgr.
[Graphical view]
PfamPF00361. Oxidored_q1. 1 hit.
PF00662. Oxidored_q1_N. 1 hit.
[Graphical view]
PRINTSPR01435. NPOXDRDTASE5.
TIGRFAMsTIGR01974. NDH_I_L. 1 hit.
ProtoNetSearch...

Entry information

Entry nameNUOL_BUCAI
AccessionPrimary (citable) accession number: P57262
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: February 19, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Buchnera aphidicola (subsp. Acyrthosiphon pisum)

Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names