P57261 (NUOK_BUCAI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADH-quinone oxidoreductase subunit K
EC=1.6.99.5

Alternative name(s):
NADH dehydrogenase I subunit K
NDH-1 subunit K

Gene names

Name:	nuoK
Ordered Locus Names:	BU163

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)

Taxonomic identifier

107806 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	100 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient By similarity. HAMAP MF_01456
Catalytic activity	NADH + quinone = NAD+ + quinol. HAMAP MF_01456
Subunit structure	NDH-1 is composed of 13 different subunits. Subunits NuoA, H, J, K, L, M, N constitute the membrane sector of the complex By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity HAMAP MF_01456.
Sequence similarities	Belongs to the complex I subunit 4L family.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	NAD Ubiquinone
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	ATP synthesis coupled electron transport Inferred from electronic annotation. Source: InterPro transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NADH dehydrogenase (quinone) activity Inferred from electronic annotation. Source: EC quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 100	100	NADH-quinone oxidoreductase subunit K HAMAP MF_01456		PRO_0000118528
Regions
	Transmembrane	4 – 24	21	Helical; Potential
	Transmembrane	28 – 48	21	Helical; Potential
	Transmembrane	60 – 80	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	P57261 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: CA9DFE6521980052	FASTA	100	11,180
	10 20 30 40 50 60 MISLFHGLFL SLILFILGLT SLIVRRNILF ILISLEIMMN AVGLALIVVG SYWHQADGQI 70 80 90 100 MYIFVITLAA SEASIALALL LQLYRRKKTL NIDILSEMNG

References

[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed: 10993077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: APS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000003 Genomic DNA. Translation: BAB12881.1.
RefSeq	NP_239995.1. NC_002528.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBUCT00000002957; EBBUCP00000002733; EBBUCG00000002957.
GeneID	1109607.
GenomeReviews	Gene locus BU163 in contig BA000003_GR.
KEGG	buc:BU163.
PATRIC	21243842. VBIBucAph127364_0173.
Phylogenomic databases
GeneTree	EBGT00050000007928.
HOGENOM	HBG673066.
OMA	IPMEHGL.
PhylomeDB	P57261.
ProtClustDB	CLSK315606.
Enzyme and pathway databases
BioCyc	BSP107806:BU163-MONOMER.
Family and domain databases
HAMAP	MF_01456. NDH1_NuoK. [Tree]
InterPro	IPR001133. NADH_UbQ_OxRdtase_chain4L/K. [Graphical view]
KO	K00340.
PANTHER	PTHR11434. Oxidored_4L. 1 hit.
Pfam	PF00420. Oxidored_q2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NUOK_BUCAI

Accession

Primary (citable) accession number: P57261

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	January 25, 2012
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program