P57260 (NUOJ_BUCAI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADH-quinone oxidoreductase subunit J
EC=1.6.99.5

Alternative name(s):
NADH dehydrogenase I subunit J
NDH-1 subunit J

Gene names

Name:	nuoJ
Ordered Locus Names:	BU162

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)

Taxonomic identifier

107806 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	170 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient By similarity.
Catalytic activity	NADH + quinone = NAD+ + quinol.
Subunit structure	Composed of 13 different subunits. Subunits NuoA, H, J, K, L, M, N constitute the membrane sector of the complex By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein Potential.
Sequence similarities	Belongs to the complex I subunit 6 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	NAD
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 170	170	NADH-quinone oxidoreductase subunit J		PRO_0000118374
Regions
	Transmembrane	1 – 21	21	Helical; Potential
	Transmembrane	30 – 50	21	Helical; Potential
	Transmembrane	56 – 76	21	Helical; Potential
	Transmembrane	94 – 114	21	Helical; Potential
	Transmembrane	138 – 158	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	P57260 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: EA1FE307DB7AF878	FASTA	170	19,088
	10 20 30 40 50 60 MEFVFYVCSF AAVVSTFFVI IQKNAVYSLV YLIISLLSIA GVFFSLGAFF AGSLEVIIYA 70 80 90 100 110 120 GAIIVLFVFV IMMLNISDKY NLEEAHYLKP RFWIGPSILS LILLLSMTYA IFFLRDKKID 130 140 150 160 170 GFLIDSKIVG INLFGPYVFL VELSSILLLS ALVVIFHIGT EKNVDKNKVL

References

[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed: 10993077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: APS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000003 Genomic DNA. Translation: BAB12880.1.
RefSeq	NP_239994.1. NC_002528.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBUCT00000002985; EBBUCP00000002761; EBBUCG00000002985.
GeneID	1109606.
GenomeReviews	Gene locus BU162 in contig BA000003_GR.
KEGG	buc:BU162.
PATRIC	21243840. VBIBucAph127364_0172.
Phylogenomic databases
GeneTree	EBGT00050000008025.
HOGENOM	HBG729722.
OMA	IALFGPY.
PhylomeDB	P57260.
ProtClustDB	CLSK315601.
Enzyme and pathway databases
BioCyc	BSP107806:BU162-MONOMER.
Family and domain databases
InterPro	IPR001457. NADH_UbQ/plastoQ_OxRdtase_su6. [Graphical view]
KO	K00339.
Pfam	PF00499. Oxidored_q3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NUOJ_BUCAI

Accession

Primary (citable) accession number: P57260

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	January 25, 2012
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program