P57258 (NUOH_BUCAI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADH-quinone oxidoreductase subunit H
EC=1.6.99.5

Alternative name(s):
NADH dehydrogenase I subunit H
NDH-1 subunit H

Gene names

Name:	nuoH
Ordered Locus Names:	BU160

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)

Taxonomic identifier

107806 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	322 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient. This subunit may bind ubiquinone By similarity. HAMAP MF_01350
Catalytic activity	NADH + quinone = NAD+ + quinol. HAMAP MF_01350
Subunit structure	NDH-1 is composed of 13 different subunits. Subunits NuoA, H, J, K, L, M, N constitute the membrane sector of the complex By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein HAMAP MF_01350.
Sequence similarities	Belongs to the complex I subunit 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	NAD Ubiquinone
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NADH dehydrogenase (quinone) activity Inferred from electronic annotation. Source: EC quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 322	322	NADH-quinone oxidoreductase subunit H HAMAP MF_01350		PRO_0000117526
Regions
	Transmembrane	15 – 35	21	Helical; Potential
	Transmembrane	50 – 69	20	Helical; Potential
	Transmembrane	81 – 101	21	Helical; Potential
	Transmembrane	114 – 134	21	Helical; Potential
	Transmembrane	149 – 169	21	Helical; Potential
	Transmembrane	186 – 206	21	Helical; Potential
	Transmembrane	237 – 257	21	Helical; Potential
	Transmembrane	265 – 285	21	Helical; Potential
	Transmembrane	302 – 322	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	P57258 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: F251144E9A66AB52	FASTA	322	36,972
	10 20 30 40 50 60 MIWLEENMIK ITFCFFKVIF ILLLIVFSSA MLSIVERRLL AVFQNRYGPN RVGWMGSLQL 70 80 90 100 110 120 CADMIKILFK EDWIPPFSRK FIFVLSPVIA FTSLLCVIPI IPFTSHFVII DLNIGILFFL 130 140 150 160 170 180 MMASLSVYAI LFAGWSSNNK YALLGAMRAC VQTLSYEVFL GLSLMGVVAQ SGSFKISDIV 190 200 210 220 230 240 NSQKYIWNVF PQFFGFLTFL IAGLAVCHRH PFDQPESEQE LADGYHIEYS GMKFGLFFIG 250 260 270 280 290 300 EYISIITVSS LIVTLFFGGW LGPWIPSCIW FILKIIFFIF LFILIRAALP RPRYDQVLLF 310 320 GWKFCLPLTL FNLFLTAFLI LV

References

[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed: 10993077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: APS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000003 Genomic DNA. Translation: BAB12878.1.
RefSeq	NP_239992.1. NC_002528.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBUCT00000002782; EBBUCP00000002558; EBBUCG00000002782.
GeneID	1109604.
GenomeReviews	Gene locus BU160 in contig BA000003_GR.
KEGG	buc:BU160.
PATRIC	21243836. VBIBucAph127364_0170.
Phylogenomic databases
GeneTree	EBGT00050000007740.
HOGENOM	HBG727670.
OMA	MSWLTPE.
PhylomeDB	P57258.
ProtClustDB	PRK06076.
Enzyme and pathway databases
BioCyc	BSP107806:BU160-MONOMER.
Family and domain databases
HAMAP	MF_01350. NDH1_NuoH. [Tree]
InterPro	IPR001694. NADH_UbQ_OxRdtase_su1/FPO. IPR018086. NADH_UbQ_OxRdtase_su1_CS. [Graphical view]
KO	K00337.
PANTHER	PTHR11432. Resp_NADH_DH_1. 1 hit.
Pfam	PF00146. NADHdh. 1 hit. [Graphical view]
PROSITE	PS00667. COMPLEX1_ND1_1. 1 hit. PS00668. COMPLEX1_ND1_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NUOH_BUCAI

Accession

Primary (citable) accession number: P57258

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	January 25, 2012
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program