Reviewed, UniProtKB/Swiss-Prot P57256 (NUOF_BUCAI)

Names and origin

Protein names

Recommended name:
    NADH-quinone oxidoreductase subunit F
    EC=1.6.99.5
Alternative name(s):
    NADH dehydrogenase I subunit F
    NDH-1 subunit F

Gene names

Name:	nuoF
Ordered Locus Names:	BU158

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (Acyrthosiphon pisum symbiotic bacterium) [Complete proteome] [HAMAP]

Taxonomic identifier

118099 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	444 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient By similarity.
Catalytic activity	NADH + quinone = NAD+ + quinol.
Cofactor	Binds 1 FMN Potential. Binds 1 4Fe-4S cluster Potential.
Subunit structure	Composed of 13 different subunits. Subunits nuoCD, E, F, and G constitute the peripheral sector of the complex By similarity.
Sequence similarities	Belongs to the complex I 51 kDa subunit family.

Ontologies

Keywords
Ligand	4Fe-4S FMN Flavoprotein Iron Iron-sulfur Metal-binding NAD
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW FMN binding Inferred from electronic annotation. Source: InterPro NAD or NADH binding Inferred from electronic annotation. Source: InterPro NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 444	444	NADH-quinone oxidoreductase subunit F		PRO_0000118571
Regions
	Nucleotide binding	61 – 70	10	NAD By similarity
	Nucleotide binding	176 – 223	48	FMN By similarity
Sites
	Metal binding	353	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	356	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	359	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	400	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	P57256-1 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: 8E200410C135CDFC	FASTA	444	49,595
	10 20 30 40 50 60 MNRILRISET HPLTWRLRDD QKTVWIKEYC DKNGYLSLKK ALKEMLPEDV INIVKESGLK 70 80 90 100 110 120 GRGGAGFSTG LKWSLMSQNH SYTRERGYLI CNADEMEPGT YKDRLLIEKI PHQLIEGIIL 130 140 150 160 170 180 SAYALNVSRA YIFLRGEYVQ AEHILKQSIQ EAINFGFIGL NILGSNFNIE LVLHTGAGRY 190 200 210 220 230 240 ICGEETALIN SLEGRRANPR SKPPFPAVFG LWGKPTCVNN VETLSNVPCI ILNGVSWYKN 250 260 270 280 290 300 LSKSSDTGTK LMGFSGNVRN PGVWELPFGT TAREILEDYA HGMKSGFSLK AWQPGGAGTD 310 320 330 340 350 360 FLLEEHLDLP MDFKNISQAG SRLGTALSMA VDNKTSMVSL VYNIEKFFSR ESCGLCTPCR 370 380 390 400 410 420 DGLPWIVKIL KSLEQNKGHK NDVKNLEKLC SHLSPGKTFC AHAPGAIEPL QSAIKYFRSE 430 440 FESGINIRKR DLNKKIIGIQ SNCI

References

[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed: 10993077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tokyo 1998.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000003 Genomic DNA. Translation: BAB12876.1.
RefSeq	NP_239990.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1109602.
GenomeReviews	Gene locus BU158 in contig BA000003_GR.
KEGG	buc:BU158.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P57256.
OMA	P57256. LPHLLIE.
Enzyme and pathway databases
BioCyc	BSP107806:BU158-MON.
Family and domain databases
InterPro	IPR001949. NADH-UbQ_OxRdtase_51KDa_CS. IPR019575. NADH-UbQ_OxRdtase_Fsu_4Fe4S-bd. IPR011537. NADH-UbQ_OxRdtase_suF. IPR011538. NADH_UbQ_OxRdtase_51KDa_su. IPR019554. Soluble_ligand_bd. [Graphical view]
Pfam	PF01512. Complex1_51K. 1 hit. PF10589. NADH_4Fe-4S. 1 hit. PF10531. SLBB. 1 hit. [Graphical view]
TIGRFAMs	TIGR01959. nuoF_fam. 1 hit.
PROSITE	PS00644. COMPLEX1_51K_1. 1 hit. PS00645. COMPLEX1_51K_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NUOF_BUCAI

Accession

Primary (citable) accession number: P57256

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	June 16, 2009
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)