P57255 (NUOE_BUCAI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADH-quinone oxidoreductase subunit E
EC=1.6.99.5

Alternative name(s):
NADH dehydrogenase I subunit E
NDH-1 subunit E

Gene names

Name:	nuoE
Ordered Locus Names:	BU157

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)

Taxonomic identifier

107806 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	162 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient By similarity.
Catalytic activity	NADH + quinone = NAD+ + quinol.
Cofactor	Binds 1 2Fe-2S cluster Potential.
Subunit structure	Composed of 13 different subunits. Subunits NuoCD, E, F, and G constitute the peripheral sector of the complex By similarity.
Sequence similarities	Belongs to the complex I 24 kDa subunit family.

Ontologies

Keywords
Ligand	2Fe-2S Iron Iron-sulfur Metal-binding NAD
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW NAD binding Inferred from electronic annotation. Source: InterPro NADH dehydrogenase (quinone) activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 162	162	NADH-quinone oxidoreductase subunit E		PRO_0000118688
Sites
	Metal binding	88	1	Iron-sulfur (2Fe-2S) Potential
	Metal binding	93	1	Iron-sulfur (2Fe-2S) Potential
	Metal binding	129	1	Iron-sulfur (2Fe-2S) Potential
	Metal binding	133	1	Iron-sulfur (2Fe-2S) Potential

Sequences

	Sequence		Length	Mass (Da)
	P57255 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: 428DC8DBA0B463F1	FASTA	162	18,598
	10 20 30 40 50 60 MQEISIKFKL TNEEINAIEN QKKYYEDFRA ISIEALKIVQ KKRGWVSDQA IYAIAEILHI 70 80 90 100 110 120 NPSDVEGVAT FYSQIFRKPV GRNIIRYCDS VVCFLTGYKR IQIALENYLK IKIGETTKDD 130 140 150 160 RFTLLPVCCL GNCDKGPTIM INEDTYSVLT PESIPSLLES YK

References

[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed: 10993077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: APS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000003 Genomic DNA. Translation: BAB12875.1.
RefSeq	NP_239989.1. NC_002528.1.
3D structure databases
ProteinModelPortal	P57255.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBUCT00000003278; EBBUCP00000003054; EBBUCG00000003278.
GeneID	1109601.
GenomeReviews	Gene locus BU157 in contig BA000003_GR.
KEGG	buc:BU157.
PATRIC	21243830. VBIBucAph127364_0167.
Phylogenomic databases
GeneTree	EBGT00050000008166.
HOGENOM	HBG616790.
OMA	IVQKERG.
PhylomeDB	P57255.
ProtClustDB	PRK07539.
Enzyme and pathway databases
BioCyc	BSP107806:BU157-MONOMER.
Family and domain databases
InterPro	IPR002023. NADH_UbQ_OxRdtase_24kDa_su. IPR012336. Thioredoxin-like_fold. [Graphical view]
Gene3D	G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
KO	K00334.
PANTHER	PTHR10371. Cmplx1_24kDa. 1 hit.
Pfam	PF01257. Complex1_24kDa. 1 hit. [Graphical view]
PIRSF	PIRSF000216. NADH_DH_24kDa. 1 hit.
SUPFAM	SSF52833. Thiordxn-like_fd. 1 hit.
TIGRFAMs	TIGR01958. NuoE_fam. 1 hit.
PROSITE	PS01099. COMPLEX1_24K. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NUOE_BUCAI

Accession

Primary (citable) accession number: P57255

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	January 25, 2012
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program