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Protein

NADH-quinone oxidoreductase subunit E

Gene

nuoE

Organism
Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient (By similarity).By similarity

Catalytic activityi

NADH + quinone = NAD+ + quinol.

Cofactori

[2Fe-2S] clusterCuratedNote: Binds 1 [2Fe-2S] cluster.Curated

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi88 – 881Iron-sulfur (2Fe-2S)Sequence Analysis
Metal bindingi93 – 931Iron-sulfur (2Fe-2S)Sequence Analysis
Metal bindingi129 – 1291Iron-sulfur (2Fe-2S)Sequence Analysis
Metal bindingi133 – 1331Iron-sulfur (2Fe-2S)Sequence Analysis

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. NADH dehydrogenase (quinone) activity Source: UniProtKB-EC
  4. quinone binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

2Fe-2S, Iron, Iron-sulfur, Metal-binding, NAD

Enzyme and pathway databases

BioCyciBAPH107806:GBZJ-157-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
NADH-quinone oxidoreductase subunit E (EC:1.6.99.5)
Alternative name(s):
NADH dehydrogenase I subunit E
NDH-1 subunit E
Gene namesi
Name:nuoE
Ordered Locus Names:BU157
OrganismiBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Taxonomic identifieri107806 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
ProteomesiUP000001806 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 162162NADH-quinone oxidoreductase subunit EPRO_0000118688Add
BLAST

Keywords - PTMi

Quinone

Interactioni

Subunit structurei

Composed of 13 different subunits. Subunits NuoCD, E, F, and G constitute the peripheral sector of the complex (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliP57255.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the complex I 24 kDa subunit family.Curated

Phylogenomic databases

eggNOGiCOG1905.
HOGENOMiHOG000257749.
KOiK00334.
OMAiGTACHVF.
OrthoDBiEOG6T4S12.

Family and domain databases

Gene3Di3.40.30.10. 1 hit.
InterProiIPR002023. NuoE-like.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PIRSFiPIRSF000216. NADH_DH_24kDa. 1 hit.
SUPFAMiSSF52833. SSF52833. 1 hit.
TIGRFAMsiTIGR01958. nuoE_fam. 1 hit.
PROSITEiPS01099. COMPLEX1_24K. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P57255-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQEISIKFKL TNEEINAIEN QKKYYEDFRA ISIEALKIVQ KKRGWVSDQA
60 70 80 90 100
IYAIAEILHI NPSDVEGVAT FYSQIFRKPV GRNIIRYCDS VVCFLTGYKR
110 120 130 140 150
IQIALENYLK IKIGETTKDD RFTLLPVCCL GNCDKGPTIM INEDTYSVLT
160
PESIPSLLES YK
Length:162
Mass (Da):18,598
Last modified:November 30, 2000 - v1
Checksum:i428DC8DBA0B463F1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000003 Genomic DNA. Translation: BAB12875.1.
RefSeqiNP_239989.1. NC_002528.1.

Genome annotation databases

EnsemblBacteriaiBAB12875; BAB12875; BAB12875.
GeneIDi1109601.
KEGGibuc:BU157.
PATRICi21243830. VBIBucAph127364_0167.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000003 Genomic DNA. Translation: BAB12875.1.
RefSeqiNP_239989.1. NC_002528.1.

3D structure databases

ProteinModelPortaliP57255.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB12875; BAB12875; BAB12875.
GeneIDi1109601.
KEGGibuc:BU157.
PATRICi21243830. VBIBucAph127364_0167.

Phylogenomic databases

eggNOGiCOG1905.
HOGENOMiHOG000257749.
KOiK00334.
OMAiGTACHVF.
OrthoDBiEOG6T4S12.

Enzyme and pathway databases

BioCyciBAPH107806:GBZJ-157-MONOMER.

Family and domain databases

Gene3Di3.40.30.10. 1 hit.
InterProiIPR002023. NuoE-like.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PIRSFiPIRSF000216. NADH_DH_24kDa. 1 hit.
SUPFAMiSSF52833. SSF52833. 1 hit.
TIGRFAMsiTIGR01958. nuoE_fam. 1 hit.
PROSITEiPS01099. COMPLEX1_24K. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
    Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
    Nature 407:81-86(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: APS.

Entry informationi

Entry nameiNUOE_BUCAI
AccessioniPrimary (citable) accession number: P57255
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 30, 2000
Last sequence update: November 30, 2000
Last modified: March 31, 2015
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Buchnera aphidicola (subsp. Acyrthosiphon pisum)
    Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.