Reviewed, UniProtKB/Swiss-Prot P57254 (NUOCD_BUCAI)

Names and origin

Protein names

Recommended name:
    NADH-quinone oxidoreductase subunit C/D
    EC=1.6.99.5
Alternative name(s):
    NADH dehydrogenase I subunit C/D
    NDH-1 subunit C/D

Gene names

Name:	nuoCD
Ordered Locus Names:	BU156

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (Acyrthosiphon pisum symbiotic bacterium) [Complete proteome] [HAMAP]

Taxonomic identifier

118099 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	600 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient By similarity.
Catalytic activity	NADH + quinone = NAD(+) + quinol.
Subunit structure	Composed of 13 different subunits. Subunits nuoCD, E, F, and G constitute the peripheral sector of the complex By similarity.
Sequence similarities	In the N-terminal section; belongs to the complex I 30 kDa subunit family. In the C-terminal section; belongs to the complex I 49 kDa subunit family.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	mitochondrial electron transport, NADH to ubiquinone Inferred from electronic annotation. Source: InterPro
Molecular function	NAD binding Inferred from electronic annotation. Source: InterPro NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 600	600	NADH-quinone oxidoreductase subunit C/D		PRO_0000118681
Regions
	Region	1 – 190	190	NADH dehydrogenase I subunit C
	Region	214 – 600	387	NADH dehydrogenase I subunit D

Sequences

	Sequence		Length	Mass (Da)
	P57254-1 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: 033AA3AFAC5C567D	FASTA	600	69,576
	10 20 30 40 50 60 MIDLMPKKNT FLLKKKYKED SNNSVINNLF DFFGKEFCFH QITLTGFPII WIDKTLLIEV 70 80 90 100 110 120 GKFLYHLSQP YIMLYDLHGV DERIRLHRED LPKADFSVFY HLISIERNSD IMIKVPLLEN 130 140 150 160 170 180 DLILPTFTGL FPNANWYERE TWDMFGIIFN NHPNLTRIIM PSTWKGHPLR KNYSARATEY 190 200 210 220 230 240 EPFFLNEQKE DLEMEGLKFK PELWGMKRKN DNVEFMFLNL GPNHPSAHGA FRIVLQLDGE 250 260 270 280 290 300 NIVDCVPDIG YHHRGAEKMA ERQSWHSYIP YTDRIEYLGG CVNELPYVLA VEKLANISVP 310 320 330 340 350 360 EKAEVIRVMM SELFRINSHL LYISTFIQDV GCMTPVFFAF TDRQKIYDLI EAITGARMHP 370 380 390 400 410 420 AWFRIGGVAN DLPQGWNILL KEFLDWMPKR LKYYINTALK NSILIHRSKG IAEYNKKEAL 430 440 450 460 470 480 QWGVTGAGLR ATGLNFDVRK WRPYSGYQNY TFEVPVGSGI SDCYSRVMIK VEEIYQSLFI 490 500 510 520 530 540 LKQCLCNMPS GPFKSEDSLT TPPSKECVLQ NIETMITHFL QVSWGPVIPE NESFQMIEAT 550 560 570 580 590 600 KGINSYYLIS DGGTMSYRTR IRTPSFPHLQ QIPSVIRGSL ISDLIVYLGS IDFVMSDVDR

References

[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed: 10993077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tokyo 1998.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000003 Genomic DNA. Translation: BAB12874.1.
RefSeq	NP_239988.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1109600.
GenomeReviews	Gene locus BU156 in contig BA000003_GR.
KEGG	buc:BU156.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P57254.
Enzyme and pathway databases
BioCyc	BSP107806:BU156-MON.
Family and domain databases
InterPro	IPR014029. NADH-UbQ_OxRdtase_49kDa_CS. IPR010219. NADH_DH_1_dsu. IPR010218. NADH_DH_csu. IPR001268. NADH_DHase_Ub_30kDa_su. IPR001135. NADH_UbQ_OxRdtase_49kDa. [Graphical view]
Pfam	PF00329. Complex1_30kDa. 1 hit. PF00346. Complex1_49kDa. 1 hit. [Graphical view]
ProDom	PD001581. Complex1_30K. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01961. NuoC_fam. 1 hit. TIGR01962. NuoD. 1 hit.
PROSITE	PS00542. COMPLEX1_30K. False negative. PS00535. COMPLEX1_49K. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NUOCD_BUCAI

Accession

Primary (citable) accession number: P57254

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	November 25, 2008
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)