P57253 (NUOB_BUCAI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADH-quinone oxidoreductase subunit B
EC=1.6.99.5

Alternative name(s):
NADH dehydrogenase I subunit B
NDH-1 subunit B

Gene names

Name:	nuoB
Ordered Locus Names:	BU155

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium) [Reference proteome] [HAMAP]

Taxonomic identifier

107806 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera › Buchnera aphidicola › Buchnera aphidicola (Acyrthosiphon pisum)

Protein attributes

Sequence length	224 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient By similarity. HAMAP-Rule MF_01356
Catalytic activity	NADH + quinone = NAD+ + quinol. HAMAP-Rule MF_01356
Cofactor	Binds 1 4Fe-4S cluster By similarity. HAMAP-Rule MF_01356
Subunit structure	NDH-1 is composed of 13 different subunits. Subunits NuoB, CD, E, F, and G constitute the peripheral sector of the complex By similarity.
Subcellular location	Cell membrane; Peripheral membrane protein; Cytoplasmic side By similarity HAMAP-Rule MF_01356.
Sequence similarities	Belongs to the complex I 20 kDa subunit family.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell membrane Membrane
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding NAD Ubiquinone
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	photosynthesis, light reaction Inferred from electronic annotation. Source: HAMAP transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: HAMAP quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 224	224	NADH-quinone oxidoreductase subunit B HAMAP-Rule MF_01356		PRO_0000118769
Sites
	Metal binding	67	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	68	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	133	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	162	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	P57253 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: BB450B102C87CD7B	FASTA	224	25,597
	10 20 30 40 50 60 MNYTLTKADS DNNNKKYPKQ TIESVSDPLE EYLKKNIFMG KITQLLHKLV NWGRKNSLWP 70 80 90 100 110 120 YNFGLSCCYV EMVSAFTSVH DVARFGSEVL RASPRQADVM VIAGTPFIKM APVIQRLYDQ 130 140 150 160 170 180 MLEPKWVISM GACANSGGMY DIYSVVQGVD KFLPVDIYIP GCPPRPEAYM QALILLQKLI 190 200 210 220 NEERRPLSWV IGEQGVYHKK MPSERVQKRS KRINIINLST SEKI

References

[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: APS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000003 Genomic DNA. Translation: BAB12873.1.
RefSeq	NP_239987.1. NC_002528.1.
3D structure databases
ProteinModelPortal	P57253.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAB12873; BAB12873; BAB12873.
GeneID	1109599.
KEGG	buc:BU155.
PATRIC	21243826. VBIBucAph127364_0165.
Phylogenomic databases
eggNOG	COG0377.
HOGENOM	HOG000228249.
KO	K00331.
OMA	PRPEAYI.
ProtClustDB	PRK06411.
Enzyme and pathway databases
BioCyc	BAPH107806:GBZJ-155-MONOMER.
Family and domain databases
Gene3D	3.40.50.700. 1 hit.
HAMAP	MF_01356. NDH1_NuoB.
InterPro	IPR006137. NADH_UbQ_OxRdtase-like_20kDa. IPR006138. NADH_UQ_OxRdtase_20Kd_su. IPR014406. NiFe-hyd_3_ssu/Q_oxred_NuoB. [Graphical view]
PANTHER	PTHR11995. PTHR11995. 1 hit.
Pfam	PF01058. Oxidored_q6. 1 hit. [Graphical view]
TIGRFAMs	TIGR01957. nuoB_fam. 1 hit.
PROSITE	PS01150. COMPLEX1_20K. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NUOB_BUCAI

Accession

Primary (citable) accession number: P57253

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	May 29, 2013
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program