Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P57253

- NUOB_BUCAI

UniProt

P57253 - NUOB_BUCAI

Protein

NADH-quinone oxidoreductase subunit B

Gene

nuoB

Organism
Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 81 (01 Oct 2014)
      Sequence version 1 (01 Dec 2000)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient By similarity.By similarity

    Catalytic activityi

    NADH + quinone = NAD+ + quinol.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi67 – 671Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi68 – 681Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi133 – 1331Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi162 – 1621Iron-sulfur (4Fe-4S)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    2. iron ion binding Source: UniProtKB-HAMAP
    3. NADH dehydrogenase (ubiquinone) activity Source: InterPro
    4. quinone binding Source: UniProtKB-KW

    GO - Biological processi

    1. transport Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Transport

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

    Enzyme and pathway databases

    BioCyciBAPH107806:GBZJ-155-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NADH-quinone oxidoreductase subunit BUniRule annotation (EC:1.6.99.5UniRule annotation)
    Alternative name(s):
    NADH dehydrogenase I subunit BUniRule annotation
    NDH-1 subunit BUniRule annotation
    Gene namesi
    Name:nuoBUniRule annotation
    Ordered Locus Names:BU155
    OrganismiBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
    Taxonomic identifieri107806 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
    ProteomesiUP000001806: Chromosome

    Subcellular locationi

    Cell membrane UniRule annotation; Peripheral membrane protein UniRule annotation; Cytoplasmic side UniRule annotation

    GO - Cellular componenti

    1. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 224224NADH-quinone oxidoreductase subunit BPRO_0000118769Add
    BLAST

    Keywords - PTMi

    Quinone

    Interactioni

    Subunit structurei

    NDH-1 is composed of 13 different subunits. Subunits NuoB, CD, E, F, and G constitute the peripheral sector of the complex.UniRule annotation

    Structurei

    3D structure databases

    ProteinModelPortaliP57253.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the complex I 20 kDa subunit family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0377.
    HOGENOMiHOG000228249.
    KOiK00331.
    OMAiPRPEAYI.
    OrthoDBiEOG62K20C.

    Family and domain databases

    Gene3Di3.40.50.700. 1 hit.
    HAMAPiMF_01356. NDH1_NuoB.
    InterProiIPR006137. NADH_UbQ_OxRdtase-like_20kDa.
    IPR006138. NADH_UQ_OxRdtase_20Kd_su.
    [Graphical view]
    PfamiPF01058. Oxidored_q6. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR01957. nuoB_fam. 1 hit.
    PROSITEiPS01150. COMPLEX1_20K. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P57253-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNYTLTKADS DNNNKKYPKQ TIESVSDPLE EYLKKNIFMG KITQLLHKLV    50
    NWGRKNSLWP YNFGLSCCYV EMVSAFTSVH DVARFGSEVL RASPRQADVM 100
    VIAGTPFIKM APVIQRLYDQ MLEPKWVISM GACANSGGMY DIYSVVQGVD 150
    KFLPVDIYIP GCPPRPEAYM QALILLQKLI NEERRPLSWV IGEQGVYHKK 200
    MPSERVQKRS KRINIINLST SEKI 224
    Length:224
    Mass (Da):25,597
    Last modified:December 1, 2000 - v1
    Checksum:iBB450B102C87CD7B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000003 Genomic DNA. Translation: BAB12873.1.
    RefSeqiNP_239987.1. NC_002528.1.

    Genome annotation databases

    EnsemblBacteriaiBAB12873; BAB12873; BAB12873.
    GeneIDi1109599.
    KEGGibuc:BU155.
    PATRICi21243826. VBIBucAph127364_0165.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000003 Genomic DNA. Translation: BAB12873.1 .
    RefSeqi NP_239987.1. NC_002528.1.

    3D structure databases

    ProteinModelPortali P57253.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAB12873 ; BAB12873 ; BAB12873 .
    GeneIDi 1109599.
    KEGGi buc:BU155.
    PATRICi 21243826. VBIBucAph127364_0165.

    Phylogenomic databases

    eggNOGi COG0377.
    HOGENOMi HOG000228249.
    KOi K00331.
    OMAi PRPEAYI.
    OrthoDBi EOG62K20C.

    Enzyme and pathway databases

    BioCyci BAPH107806:GBZJ-155-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.700. 1 hit.
    HAMAPi MF_01356. NDH1_NuoB.
    InterProi IPR006137. NADH_UbQ_OxRdtase-like_20kDa.
    IPR006138. NADH_UQ_OxRdtase_20Kd_su.
    [Graphical view ]
    Pfami PF01058. Oxidored_q6. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR01957. nuoB_fam. 1 hit.
    PROSITEi PS01150. COMPLEX1_20K. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
      Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
      Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: APS.

    Entry informationi

    Entry nameiNUOB_BUCAI
    AccessioniPrimary (citable) accession number: P57253
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: December 1, 2000
    Last modified: October 1, 2014
    This is version 81 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Buchnera aphidicola (subsp. Acyrthosiphon pisum)
      Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3