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P57253 (NUOB_BUCAI) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NADH-quinone oxidoreductase subunit B

EC=1.6.99.5
Alternative name(s):
NADH dehydrogenase I subunit B
NDH-1 subunit B
Gene names
Name:nuoB
Ordered Locus Names:BU155
OrganismBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium) [Reference proteome] [HAMAP]
Taxonomic identifier107806 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera

Protein attributes

Sequence length224 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient By similarity. HAMAP-Rule MF_01356

Catalytic activity

NADH + quinone = NAD+ + quinol. HAMAP-Rule MF_01356

Cofactor

Binds 1 4Fe-4S cluster By similarity. HAMAP-Rule MF_01356

Subunit structure

NDH-1 is composed of 13 different subunits. Subunits NuoB, CD, E, F, and G constitute the peripheral sector of the complex By similarity.

Subcellular location

Cell membrane; Peripheral membrane protein; Cytoplasmic side By similarity HAMAP-Rule MF_01356.

Sequence similarities

Belongs to the complex I 20 kDa subunit family.

Ontologies

Keywords
   Biological processTransport
   Cellular componentCell membrane
Membrane
   Ligand4Fe-4S
Iron
Iron-sulfur
Metal-binding
NAD
Ubiquinone
   Molecular functionOxidoreductase
   PTMQuinone
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processtransport

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentplasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_function4 iron, 4 sulfur cluster binding

Inferred from electronic annotation. Source: UniProtKB-KW

NADH dehydrogenase (ubiquinone) activity

Inferred from electronic annotation. Source: InterPro

iron ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

quinone binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 224224NADH-quinone oxidoreductase subunit B HAMAP-Rule MF_01356
PRO_0000118769

Sites

Metal binding671Iron-sulfur (4Fe-4S) Potential
Metal binding681Iron-sulfur (4Fe-4S) Potential
Metal binding1331Iron-sulfur (4Fe-4S) Potential
Metal binding1621Iron-sulfur (4Fe-4S) Potential

Sequences

Sequence LengthMass (Da)Tools
P57253 [UniParc].

Last modified December 1, 2000. Version 1.
Checksum: BB450B102C87CD7B

FASTA22425,597
        10         20         30         40         50         60 
MNYTLTKADS DNNNKKYPKQ TIESVSDPLE EYLKKNIFMG KITQLLHKLV NWGRKNSLWP 

        70         80         90        100        110        120 
YNFGLSCCYV EMVSAFTSVH DVARFGSEVL RASPRQADVM VIAGTPFIKM APVIQRLYDQ 

       130        140        150        160        170        180 
MLEPKWVISM GACANSGGMY DIYSVVQGVD KFLPVDIYIP GCPPRPEAYM QALILLQKLI 

       190        200        210        220 
NEERRPLSWV IGEQGVYHKK MPSERVQKRS KRINIINLST SEKI 

« Hide

References

[1]"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: APS.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000003 Genomic DNA. Translation: BAB12873.1.
RefSeqNP_239987.1. NC_002528.1.

3D structure databases

ProteinModelPortalP57253.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAB12873; BAB12873; BAB12873.
GeneID1109599.
KEGGbuc:BU155.
PATRIC21243826. VBIBucAph127364_0165.

Phylogenomic databases

eggNOGCOG0377.
HOGENOMHOG000228249.
KOK00331.
OMAPRPEAYI.
OrthoDBEOG62K20C.
ProtClustDBPRK06411.

Enzyme and pathway databases

BioCycBAPH107806:GBZJ-155-MONOMER.

Family and domain databases

Gene3D3.40.50.700. 1 hit.
HAMAPMF_01356. NDH1_NuoB.
InterProIPR006137. NADH_UbQ_OxRdtase-like_20kDa.
IPR006138. NADH_UQ_OxRdtase_20Kd_su.
[Graphical view]
PfamPF01058. Oxidored_q6. 1 hit.
[Graphical view]
TIGRFAMsTIGR01957. nuoB_fam. 1 hit.
PROSITEPS01150. COMPLEX1_20K. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNUOB_BUCAI
AccessionPrimary (citable) accession number: P57253
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: February 19, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Buchnera aphidicola (subsp. Acyrthosiphon pisum)

Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names