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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).

Cofactori

Zn2+Note: Binds 1 zinc ion per subunit.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei563Aminoacyl-adenylate1
Binding sitei607ATP1
Metal bindingi903Zinc1
Metal bindingi906Zinc1
Metal bindingi923Zinc1
Metal bindingi926Zinc1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:BU149
OrganismiBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Taxonomic identifieri107806 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesErwiniaceaeBuchnera
Proteomesi
  • UP000001806 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000983651 – 940Isoleucine--tRNA ligaseAdd BLAST940

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

STRINGi107806.BU149.

Structurei

3D structure databases

ProteinModelPortaliP57249.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi58 – 68"HIGH" regionAdd BLAST11
Motifi604 – 608"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.

Phylogenomic databases

eggNOGiENOG4105C07. Bacteria.
COG0060. LUCA.
HOGENOMiHOG000246402.
KOiK01870.
OMAiATEQWFI.

Family and domain databases

CDDicd07960. Anticodon_Ia_Ile_BEm. 1 hit.
Gene3Di3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1. 1 hit.
InterProiView protein in InterPro
IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR033708. Anticodon_Ile_BEm.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_Ia_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_FPG/IleRS.
PfamiView protein in Pfam
PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiView protein in PROSITE
PS00178. AA_TRNA_LIGASE_I. 1 hit.

Sequencei

Sequence statusi: Complete.

P57249-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDDYKDTLNL PKTLFSMRGN LSKKEPNILK SWNENNLYKL IRKKNQEKKI
60 70 80 90 100
FFLHDGPPYA NGNIHIGHAV NKILKDIIIK SKNMSGFDAP YIPSWDCHGL
110 120 130 140 150
PIEQKVEEKI KSNQGEISTT EFQEKCRKYA QDQVEKQKKD FIRLGVIGDW
160 170 180 190 200
DNPHLTMNFK NEANIIKTLS KIVQKKHLYQ DFKPIHWCLK CASSLSEAEI
210 220 230 240 250
EYSKKKSDSI IVGFKFKYKS IIEKLFDFQI SNKKEIHLLI WTTTPWTLPS
260 270 280 290 300
SKAISIHPDF QYQLIETERC YLIIAKELVE KTLNTLKIKK SIIRNYVKGR
310 320 330 340 350
FLEKMICLHP FLKNIDLPVI LGKHVTLESG TGAVHTAPDH GLEDYIISQK
360 370 380 390 400
YNIKTSNIVN FKGEYISNTH DKLDGVNVLE ANSIIIELLI KNNTFFHHES
410 420 430 440 450
LIHSYPHCWR HKSPVIYRAT PQWFIDIDQK QLRIKLLQEI KKVRWIPEWG
460 470 480 490 500
ESRIGEMIKK RPDWCISRQR KWGVPMSIFI HKNTRKIHPN TFVFMKKIAK
510 520 530 540 550
KVELEGLQVW WNIDSKEILG EEYQSYEKIL DILDVWFESG NTHTTINYKN
560 570 580 590 600
KNYTKKNADM FLEGSDQHRG WFMSSLIIST LISEKKPYSE VLTHGFVVDG
610 620 630 640 650
KGQKMSKSIG NTISPNEIVD TLGADILRLW VASSNYSNDI SISNEILKSS
660 670 680 690 700
SDIYRRIRNT ARFMLANISD FDPKKNIISK ENMVLLDKWA IGQTKIVQEE
710 720 730 740 750
IIQHYNNYNF HAVIQRLMYF CSIEMGSFYL DIIKDRQYTL KKHSQERRSS
760 770 780 790 800
QTAIYYIINS LVRWIAPILS FTADEIWSYL PENNSQYVFM EEWFDKLFYL
810 820 830 840 850
DQDDLFNYQF WNEIITIKHE INKFLEEAIQ NKTINNSLET SIILYVSHEL
860 870 880 890 900
SNKLKILEQE TKFIFLTSDI QIKLYDTAPK NAKKSKIVPY LKVSLEKIKG
910 920 930 940
KKCPRCWHYF NFTKKNIKNS DICNRCILNT IGNGEKRIFI
Length:940
Mass (Da):110,457
Last modified:December 1, 2000 - v1
Checksum:i769F5DC1504F6496
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000003 Genomic DNA. Translation: BAB12867.1.
RefSeqiNP_239981.1. NC_002528.1.
WP_010895974.1. NC_002528.1.

Genome annotation databases

EnsemblBacteriaiBAB12867; BAB12867; BAB12867.
GeneIDi1109593.
KEGGibuc:BU149.
PATRICifig|107806.10.peg.158.

Similar proteinsi

Entry informationi

Entry nameiSYI_BUCAI
AccessioniPrimary (citable) accession number: P57249
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: November 22, 2017
This is version 112 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Buchnera aphidicola (subsp. Acyrthosiphon pisum)
    Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families