Reviewed, UniProtKB/Swiss-Prot P57245 (CARA_BUCAI)

Names and origin

Protein names

Recommended name:
    Carbamoyl-phosphate synthase small chain
    EC=6.3.5.5
Alternative name(s):
    Carbamoyl-phosphate synthetase glutamine chain

Gene names

Name:	carA
Ordered Locus Names:	BU145

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (Acyrthosiphon pisum symbiotic bacterium) [Complete proteome] [HAMAP]

Taxonomic identifier

118099 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	387 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate. HAMAP MF_01209
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1. HAMAP MF_01209 Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3. HAMAP MF_01209
Subunit structure	Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate By similarity. HAMAP MF_01209
Sequence similarities	Belongs to the carA family. Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis Pyrimidine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: HAMAP glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 387	387	Carbamoyl-phosphate synthase small chain HAMAP MF_01209		PRO_0000112261
Regions
	Domain	197 – 384	188	Glutamine amidotransferase type-1
	Region	1 – 193	193	CPSase HAMAP MF_01209
Sites
	Active site	273	1	Nucleophile By similarity
	Active site	357	1	By similarity
	Active site	359	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P57245-1 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: 2D5F9E8C8EAE1941	FASTA	387	43,020
	10 20 30 40 50 60 MEGVLSQLAV LVLEDGTKFH GRAIGAKGIT VGEVVFNTSI TGYQEIITDP SYSHQIVTLT 70 80 90 100 110 120 HPHIGNVGTN SNDEESSKIY IKGLIIRDLS LTASNYRNKK SFSSYLKENN IIAISDIDTR 130 140 150 160 170 180 KLTRILRTKG SQNGCIIEDK KQNYSIAYNK AKNFISLQDL DLAKKVSTKS IYNWDQGSFT 190 200 210 220 230 240 FKKNNFFSIN KQKFLFHVVV YDFGVKRNIL RMLVDRGCYL TVVPAITDPK TVLNLSPDGI 250 260 270 280 290 300 FLSNGPGDPR PCDYAIHAIQ YFLKTNIPIF GICLGHQLLA LASGAKIVKM KFGHHGGNHP 310 320 330 340 350 360 VKDIKNNRVI ITSQNHSFTV DTENLPNNIE ITHSSLFDGT LQGLSLTNKS AFSFQGHPEA 370 380 SPGPHDASYL FDYFIKLIVS QKTTLSN

References

[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed: 10993077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tokyo 1998.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000003 Genomic DNA. Translation: BAB12863.1.
RefSeq	NP_239977.1.
3D structure databases
SMR	P57245. Positions 9-384.
ModBase	Search...
Genome annotation databases
GeneID	1109589.
GenomeReviews	Gene locus BU145 in contig BA000003_GR.
KEGG	buc:BU145.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG286341.
OMA	FTYPELG.
PhylomeDB	P57245.
Enzyme and pathway databases
BioCyc	BSP107806:BU145-MONOMER.
Family and domain databases
HAMAP	MF_01209_B. CPSase_S_chain_B. [Tree]
InterPro	IPR006220. Anth_synthII. IPR001317. CarbamoylP_synth_GATase_dom. IPR006274. CarbamoylP_synth_ssu. IPR002474. CarbamoylP_synth_ssu_N. IPR011702. GATASE. IPR017926. GATASE_1. IPR000991. GATase_class1_C. [Graphical view]
PANTHER	PTHR11405:SF4. CarA_synth_small. 1 hit.
Pfam	PF00988. CPSase_sm_chain. 1 hit. PF00117. GATase. 1 hit. [Graphical view]
PRINTS	PR00097. ANTSNTHASEII. PR00099. CPSGATASE. PR00096. GATASE.
TIGRFAMs	TIGR01368. CPSaseIIsmall. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CARA_BUCAI

Accession

Primary (citable) accession number: P57245

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	February 9, 2010
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)