P57245 (CARA_BUCAI) Reviewed, UniProtKB/Swiss-Prot
Last modified
May 1, 2013.
Version 87.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Carbamoyl-phosphate synthase small chain EC=6.3.5.5 Alternative name(s): Carbamoyl-phosphate synthetase glutamine chain | ||||
| Gene names |
| ||||
| Organism | Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium) [Reference proteome] [HAMAP] | ||||
| Taxonomic identifier | 107806 [NCBI] | ||||
| Taxonomic lineage | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera › ![]() |
Protein attributes
| Sequence length | 387 AA. |
| Sequence status | Complete. |
| Protein existence | Inferred from homology |
General annotation (Comments)
| Catalytic activity | 2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate. HAMAP-Rule MF_01209 |
| Pathway | Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1. HAMAP-Rule MF_01209 Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3. HAMAP-Rule MF_01209 |
| Subunit structure | Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate By similarity. |
| Sequence similarities | Belongs to the CarA family. Contains 1 glutamine amidotransferase type-1 domain. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Amino-acid biosynthesis Arginine biosynthesis Pyrimidine biosynthesis |
| Domain | Glutamine amidotransferase |
| Ligand | ATP-binding Nucleotide-binding |
| Molecular function | Ligase |
| Technical term | Complete proteome Reference proteome |
| Gene Ontology (GO) | |
| Biological_process | 'de novo' UMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway arginine biosynthetic processInferred from electronic annotation. Source: HAMAP carbamoyl phosphate biosynthetic processInferred from electronic annotation. Source: InterPro glutamine catabolic processInferred from electronic annotation. Source: InterPro |
| Molecular_function | ATP binding Inferred from electronic annotation. Source: HAMAP carbamoyl-phosphate synthase (glutamine-hydrolyzing) activityInferred from electronic annotation. Source: HAMAP |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 387 | 387 | Carbamoyl-phosphate synthase small chain HAMAP-Rule MF_01209 | PRO_0000112261 | |||||
Regions | |||||||||
| Domain | 197 – 384 | 188 | Glutamine amidotransferase type-1 | ||||||
| Region | 1 – 193 | 193 | CPSase HAMAP-Rule MF_01209 | ||||||
Sites | |||||||||
| Active site | 273 | 1 | Nucleophile By similarity | ||||||
| Active site | 357 | 1 | By similarity | ||||||
| Active site | 359 | 1 | By similarity | ||||||
Sequences
| ||||||||||||||||||
References
| [1] | "Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: APS. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | BA000003 Genomic DNA. Translation: BAB12863.1. |
| RefSeq | NP_239977.1. NC_002528.1. |
3D structure databases | |
| ProteinModelPortal | P57245. |
| SMR | P57245. Positions 9-384. |
| ModBase | Search... |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| EnsemblBacteria | BAB12863; BAB12863; BAB12863. |
| GeneID | 1109589. |
| KEGG | buc:BU145. |
| PATRIC | 21243804. VBIBucAph127364_0154. |
Phylogenomic databases | |
| eggNOG | COG0505. |
| HOGENOM | HOG000038087. |
| KO | K01956. |
| OMA | RDSHYLF. |
| ProtClustDB | PRK12564. |
Enzyme and pathway databases | |
| UniPathway | UPA00068; UER00171. UPA00070; UER00115. |
Family and domain databases | |
| Gene3D | 3.50.30.20. 1 hit. |
| HAMAP | MF_01209. CPSase_S_chain. |
| InterPro | IPR006274. CarbamoylP_synth_ssu. IPR002474. CarbamoylP_synth_ssu_N. IPR017926. GATASE_1. [Graphical view] |
| Pfam | PF00988. CPSase_sm_chain. 1 hit. PF00117. GATase. 1 hit. [Graphical view] |
| SMART | SM01097. CPSase_sm_chain. 1 hit. [Graphical view] |
| SUPFAM | SSF52021. CP_synthsmall. 1 hit. |
| TIGRFAMs | TIGR01368. CPSaseIIsmall. 1 hit. |
| PROSITE | PS51273. GATASE_TYPE_1. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | CARA_BUCAI | ||||||||
| Accession | Primary (citable) accession number: P57245 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Prokaryotic Protein Annotation Program | ||||||||
Relevant documents
| Buchnera aphidicola (subsp. Acyrthosiphon pisum) Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

Clusters with
