P57241 (RSMA_BUCAI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal RNA small subunit methyltransferase A
EC=2.1.1.182

Alternative name(s):
16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase
16S rRNA dimethyladenosine transferase
16S rRNA dimethylase
S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase

Gene names

Name:	rsmA
Synonyms:	ksgA
Ordered Locus Names:	BU141

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium) [Reference proteome] [HAMAP]

Taxonomic identifier

107806 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera › Buchnera aphidicola › Buchnera aphidicola (Acyrthosiphon pisum)

Protein attributes

Sequence length	273 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits By similarity. HAMAP-Rule MF_00607
Catalytic activity	4 S-adenosyl-L-methionine + adenine(1518)/adenine(1519) in 16S rRNA = 4 S-adenosyl-L-homocysteine + N(6)-dimethyladenine(1518)/N(6)-dimethyladenine(1519) in 16S rRNA. HAMAP-Rule MF_00607
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_00607.
Sequence similarities	Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	RNA-binding S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	rRNA methylation Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase activity Inferred from electronic annotation. Source: EC RNA binding Inferred from electronic annotation. Source: UniProtKB-KW rRNA (adenine-N6,N6-)-dimethyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 273	273	Ribosomal RNA small subunit methyltransferase A HAMAP-Rule MF_00607		PRO_0000101499
Sites
	Binding site	17	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	19	1	S-adenosyl-L-methionine; via amide nitrogen By similarity
	Binding site	44	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	65	1	S-adenosyl-L-methionine By similarity
	Binding site	111	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P57241 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: 70B632C67DF437F9	FASTA	273	31,913
	10 20 30 40 50 60 MPKNFKKHLP LKKYGQNFLI NKDTIKNIIK IIDPKTTETL VEIGPGLAAL TKPMCELLEE 70 80 90 100 110 120 LIVIEIDQDL LFLLKKRSFY SKLIVFYQNA LNFNFLNLFH KKKQLIRIFG NLPYNISTSL 130 140 150 160 170 180 IIFLFKQIKV IQDMNFMLQK EVAERLISTP GNKSYGRLSI ISQYYCDIKI LLNVSPEDFR 190 200 210 220 230 240 PIPKVHSVFI NLKPHTNSPY FVYNVNILSA ITKDAFQNRR KILRHSLKNL FSEKELIKLE 250 260 270 INSNLRAENV SVSHYCKLAN YLYKKSNNLL KIN

References

[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: APS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000003 Genomic DNA. Translation: BAB12859.1.
RefSeq	NP_239973.1. NC_002528.1.
3D structure databases
ProteinModelPortal	P57241.
ModBase	Search...
Proteomic databases
PRIDE	P57241.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAB12859; BAB12859; BAB12859.
GeneID	1109585.
KEGG	buc:BU141.
PATRIC	21243796. VBIBucAph127364_0150.
Phylogenomic databases
eggNOG	COG0030.
HOGENOM	HOG000227962.
KO	K02528.
OMA	IANIPYN.
ProtClustDB	PRK00274.
Family and domain databases
Gene3D	1.10.8.100. 1 hit.
HAMAP	MF_00607. 16SrRNA_methyltr_A.
InterPro	IPR023165. rRNA_Ade_diMease-like. IPR020596. rRNA_Ade_Mease_Trfase_CS. IPR001737. rRNA_Ade_methylase_transferase. IPR020598. rRNA_Ade_methylase_Trfase_N. IPR011530. rRNA_adenine_dimethylase. [Graphical view]
PANTHER	PTHR11727. PTHR11727. 1 hit.
Pfam	PF00398. RrnaAD. 1 hit. [Graphical view]
SMART	SM00650. rADc. 1 hit. [Graphical view]
TIGRFAMs	TIGR00755. ksgA. 1 hit.
PROSITE	PS01131. RRNA_A_DIMETH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RSMA_BUCAI

Accession

Primary (citable) accession number: P57241

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	May 1, 2013
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program