P57235 (SYGB_BUCAI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycine--tRNA ligase beta subunit
EC=6.1.1.14

Alternative name(s):
Glycyl-tRNA synthetase beta subunit
Short name=GlyRS

Gene names

Name:	glyS
Ordered Locus Names:	BU135

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)

Taxonomic identifier

107806 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	690 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly). HAMAP MF_00255
Subunit structure	Tetramer of two alpha and two beta subunits By similarity.
Subcellular location	Cytoplasm HAMAP MF_00255.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	arginyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro glycyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW arginine-tRNA ligase activity Inferred from electronic annotation. Source: InterPro glycine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 690	690	Glycine--tRNA ligase beta subunit HAMAP MF_00255		PRO_0000072895

Sequences

	Sequence		Length	Mass (Da)
	P57235 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: 2788D93B7B231403	FASTA	690	80,803
	10 20 30 40 50 60 MTKKILLIEI GTEELPARLL SKISLYFYKN FIKELDFHNI SYKNIKYFST PRRLALKIKD 70 80 90 100 110 120 IDITERFVEI KKRGPSIINS YDKDGFLTEA ATRWLKHCGI NINQAIRLKN EKGEWLFYKT 130 140 150 160 170 180 RKKQENIESL IPKITESALK NISIKKSMRW GQDNQKFSRP IRNIVILLDK KVIPGDVFNI 190 200 210 220 230 240 TSKNLLQNHL SSKDSQIKIK DAKDYPKILL EKNNIIADYF IRKEKIIEDI ENIAKKIKGF 250 260 270 280 290 300 IKKNNVLIEE VTALVESPKA LLVNFQEKFL QIPKKILINT IEKKQKCFPI YNSEKKLLPY 310 320 330 340 350 360 FIFISNIQTQ ESEKIIIGNQ RVMHARLSDA EFFFKNDRKV KLESRLLSLK KVLFQNNLGS 370 380 390 400 410 420 LYEKTLRIKL LIKWIAKYSS SDVEDSIRAA LLSKCDLVTD VVCEFPELQG KIGMYYALED 430 440 450 460 470 480 KEKKDVATAL EEQYLPRFSG DKLPCTPIGC GLSIADKMDT LSGMFYIGNI PSSDKDPFAL 490 500 510 520 530 540 RRLAIGIIRI ILEKNIPLNL EDLIKKSLSL YNKKNEDDLI LFDKMIKFFM IRLFHWYEET 550 560 570 580 590 600 GYSAKIIKSV LSCKSIELID IHKKIQAISF FKKLKDSQSI ILSIKRISNI LAKEKEKING 610 620 630 640 650 660 DINKKLMIEK EEIILFNNIE EFDNYTKNLF LEKKYNDILI KIKSFENPIY NFFKKVKIYH 670 680 690 SDSKIRLNRL LLLSKLKKIF FKIADFSYLY

References

[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed: 10993077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: APS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000003 Genomic DNA. Translation: BAB12853.1.
RefSeq	NP_239967.1. NC_002528.1.
3D structure databases
ProteinModelPortal	P57235.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBUCT00000003306; EBBUCP00000003082; EBBUCG00000003306.
GeneID	1109579.
GenomeReviews	Gene locus BU135 in contig BA000003_GR.
KEGG	buc:BU135.
PATRIC	21243784. VBIBucAph127364_0144.
Phylogenomic databases
GeneTree	EBGT00050000007707.
HOGENOM	HBG586599.
OMA	FIRPVHT.
PhylomeDB	P57235.
ProtClustDB	PRK01233.
Enzyme and pathway databases
BioCyc	BSP107806:BU135-MONOMER.
Family and domain databases
HAMAP	MF_00255. Gly_tRNA_synth_beta. [Tree]
InterPro	IPR008909. DALR_anticod-bd. IPR006194. Gly-tRNA-synth_II_heterodimer. IPR015944. Gly-tRNA-synth_IIc_bsu. [Graphical view]
KO	K01879.
Pfam	PF05746. DALR_1. 1 hit. PF02092. tRNA_synt_2f. 1 hit. [Graphical view]
PRINTS	PR01045. TRNASYNTHGB.
TIGRFAMs	TIGR00211. GlyS. 1 hit.
PROSITE	PS50861. AA_TRNA_LIGASE_II_GLYAB. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYGB_BUCAI

Accession

Primary (citable) accession number: P57235

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	January 25, 2012
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program