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P57235 (SYGB_BUCAI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glycine--tRNA ligase beta subunit

EC=6.1.1.14
Alternative name(s):
Glycyl-tRNA synthetase beta subunit
Short name=GlyRS
Gene names
Name:glyS
Ordered Locus Names:BU135
OrganismBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium) [Reference proteome] [HAMAP]
Taxonomic identifier107806 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera

Protein attributes

Sequence length690 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly). HAMAP-Rule MF_00255

Subunit structure

Tetramer of two alpha and two beta subunits By similarity.

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00255.

Sequence similarities

Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processarginyl-tRNA aminoacylation

Inferred from electronic annotation. Source: InterPro

glycyl-tRNA aminoacylation

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

arginine-tRNA ligase activity

Inferred from electronic annotation. Source: InterPro

glycine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 690690Glycine--tRNA ligase beta subunit HAMAP-Rule MF_00255
PRO_0000072895

Sequences

Sequence LengthMass (Da)Tools
P57235 [UniParc].

Last modified December 1, 2000. Version 1.
Checksum: 2788D93B7B231403

FASTA69080,803
        10         20         30         40         50         60 
MTKKILLIEI GTEELPARLL SKISLYFYKN FIKELDFHNI SYKNIKYFST PRRLALKIKD 

        70         80         90        100        110        120 
IDITERFVEI KKRGPSIINS YDKDGFLTEA ATRWLKHCGI NINQAIRLKN EKGEWLFYKT 

       130        140        150        160        170        180 
RKKQENIESL IPKITESALK NISIKKSMRW GQDNQKFSRP IRNIVILLDK KVIPGDVFNI 

       190        200        210        220        230        240 
TSKNLLQNHL SSKDSQIKIK DAKDYPKILL EKNNIIADYF IRKEKIIEDI ENIAKKIKGF 

       250        260        270        280        290        300 
IKKNNVLIEE VTALVESPKA LLVNFQEKFL QIPKKILINT IEKKQKCFPI YNSEKKLLPY 

       310        320        330        340        350        360 
FIFISNIQTQ ESEKIIIGNQ RVMHARLSDA EFFFKNDRKV KLESRLLSLK KVLFQNNLGS 

       370        380        390        400        410        420 
LYEKTLRIKL LIKWIAKYSS SDVEDSIRAA LLSKCDLVTD VVCEFPELQG KIGMYYALED 

       430        440        450        460        470        480 
KEKKDVATAL EEQYLPRFSG DKLPCTPIGC GLSIADKMDT LSGMFYIGNI PSSDKDPFAL 

       490        500        510        520        530        540 
RRLAIGIIRI ILEKNIPLNL EDLIKKSLSL YNKKNEDDLI LFDKMIKFFM IRLFHWYEET 

       550        560        570        580        590        600 
GYSAKIIKSV LSCKSIELID IHKKIQAISF FKKLKDSQSI ILSIKRISNI LAKEKEKING 

       610        620        630        640        650        660 
DINKKLMIEK EEIILFNNIE EFDNYTKNLF LEKKYNDILI KIKSFENPIY NFFKKVKIYH 

       670        680        690 
SDSKIRLNRL LLLSKLKKIF FKIADFSYLY 

« Hide

References

[1]"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: APS.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000003 Genomic DNA. Translation: BAB12853.1.
RefSeqNP_239967.1. NC_002528.1.

3D structure databases

ProteinModelPortalP57235.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAB12853; BAB12853; BAB12853.
GeneID1109579.
KEGGbuc:BU135.
PATRIC21243784. VBIBucAph127364_0144.

Phylogenomic databases

eggNOGCOG0751.
HOGENOMHOG000264302.
KOK01879.
OMALPIPKRM.
OrthoDBEOG661H9S.

Enzyme and pathway databases

BioCycBAPH107806:GBZJ-135-MONOMER.

Family and domain databases

HAMAPMF_00255. Gly_tRNA_synth_beta.
InterProIPR008909. DALR_anticod-bd.
IPR015944. Gly-tRNA-synth_bsu.
IPR006194. Gly-tRNA-synth_heterodimer.
[Graphical view]
PfamPF05746. DALR_1. 1 hit.
PF02092. tRNA_synt_2f. 1 hit.
[Graphical view]
PRINTSPR01045. TRNASYNTHGB.
TIGRFAMsTIGR00211. glyS. 1 hit.
PROSITEPS50861. AA_TRNA_LIGASE_II_GLYAB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYGB_BUCAI
AccessionPrimary (citable) accession number: P57235
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: May 14, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Buchnera aphidicola (subsp. Acyrthosiphon pisum)

Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries