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Protein

Glycine--tRNA ligase beta subunit

Gene

glyS

Organism
Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly).

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBAPH107806:GBZJ-135-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycine--tRNA ligase beta subunit (EC:6.1.1.14)
Alternative name(s):
Glycyl-tRNA synthetase beta subunit
Short name:
GlyRS
Gene namesi
Name:glyS
Ordered Locus Names:BU135
OrganismiBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Taxonomic identifieri107806 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
ProteomesiUP000001806 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 690690Glycine--tRNA ligase beta subunitPRO_0000072895Add
BLAST

Interactioni

Subunit structurei

Tetramer of two alpha and two beta subunits.By similarity

Protein-protein interaction databases

STRINGi107806.BU135.

Structurei

3D structure databases

ProteinModelPortaliP57235.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0751.
HOGENOMiHOG000264302.
KOiK01879.
OMAiALVEWPV.
OrthoDBiEOG661H9S.

Family and domain databases

HAMAPiMF_00255. Gly_tRNA_synth_beta.
InterProiIPR008909. DALR_anticod-bd.
IPR015944. Gly-tRNA-synth_bsu.
IPR006194. Gly-tRNA-synth_heterodimer.
[Graphical view]
PfamiPF05746. DALR_1. 1 hit.
PF02092. tRNA_synt_2f. 1 hit.
[Graphical view]
PRINTSiPR01045. TRNASYNTHGB.
TIGRFAMsiTIGR00211. glyS. 1 hit.
PROSITEiPS50861. AA_TRNA_LIGASE_II_GLYAB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P57235-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKKILLIEI GTEELPARLL SKISLYFYKN FIKELDFHNI SYKNIKYFST
60 70 80 90 100
PRRLALKIKD IDITERFVEI KKRGPSIINS YDKDGFLTEA ATRWLKHCGI
110 120 130 140 150
NINQAIRLKN EKGEWLFYKT RKKQENIESL IPKITESALK NISIKKSMRW
160 170 180 190 200
GQDNQKFSRP IRNIVILLDK KVIPGDVFNI TSKNLLQNHL SSKDSQIKIK
210 220 230 240 250
DAKDYPKILL EKNNIIADYF IRKEKIIEDI ENIAKKIKGF IKKNNVLIEE
260 270 280 290 300
VTALVESPKA LLVNFQEKFL QIPKKILINT IEKKQKCFPI YNSEKKLLPY
310 320 330 340 350
FIFISNIQTQ ESEKIIIGNQ RVMHARLSDA EFFFKNDRKV KLESRLLSLK
360 370 380 390 400
KVLFQNNLGS LYEKTLRIKL LIKWIAKYSS SDVEDSIRAA LLSKCDLVTD
410 420 430 440 450
VVCEFPELQG KIGMYYALED KEKKDVATAL EEQYLPRFSG DKLPCTPIGC
460 470 480 490 500
GLSIADKMDT LSGMFYIGNI PSSDKDPFAL RRLAIGIIRI ILEKNIPLNL
510 520 530 540 550
EDLIKKSLSL YNKKNEDDLI LFDKMIKFFM IRLFHWYEET GYSAKIIKSV
560 570 580 590 600
LSCKSIELID IHKKIQAISF FKKLKDSQSI ILSIKRISNI LAKEKEKING
610 620 630 640 650
DINKKLMIEK EEIILFNNIE EFDNYTKNLF LEKKYNDILI KIKSFENPIY
660 670 680 690
NFFKKVKIYH SDSKIRLNRL LLLSKLKKIF FKIADFSYLY
Length:690
Mass (Da):80,803
Last modified:December 1, 2000 - v1
Checksum:i2788D93B7B231403
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000003 Genomic DNA. Translation: BAB12853.1.
RefSeqiNP_239967.1. NC_002528.1.
WP_009874091.1. NC_002528.1.

Genome annotation databases

EnsemblBacteriaiBAB12853; BAB12853; BAB12853.
GeneIDi1109579.
KEGGibuc:BU135.
PATRICi21243784. VBIBucAph127364_0144.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000003 Genomic DNA. Translation: BAB12853.1.
RefSeqiNP_239967.1. NC_002528.1.
WP_009874091.1. NC_002528.1.

3D structure databases

ProteinModelPortaliP57235.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi107806.BU135.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB12853; BAB12853; BAB12853.
GeneIDi1109579.
KEGGibuc:BU135.
PATRICi21243784. VBIBucAph127364_0144.

Phylogenomic databases

eggNOGiCOG0751.
HOGENOMiHOG000264302.
KOiK01879.
OMAiALVEWPV.
OrthoDBiEOG661H9S.

Enzyme and pathway databases

BioCyciBAPH107806:GBZJ-135-MONOMER.

Family and domain databases

HAMAPiMF_00255. Gly_tRNA_synth_beta.
InterProiIPR008909. DALR_anticod-bd.
IPR015944. Gly-tRNA-synth_bsu.
IPR006194. Gly-tRNA-synth_heterodimer.
[Graphical view]
PfamiPF05746. DALR_1. 1 hit.
PF02092. tRNA_synt_2f. 1 hit.
[Graphical view]
PRINTSiPR01045. TRNASYNTHGB.
TIGRFAMsiTIGR00211. glyS. 1 hit.
PROSITEiPS50861. AA_TRNA_LIGASE_II_GLYAB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
    Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
    Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: APS.

Entry informationi

Entry nameiSYGB_BUCAI
AccessioniPrimary (citable) accession number: P57235
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: July 22, 2015
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Buchnera aphidicola (subsp. Acyrthosiphon pisum)
    Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.