Reviewed, UniProtKB/Swiss-Prot P57229 (SYFA_BUCAI)

Names and origin

Protein names

Recommended name:
    Phenylalanyl-tRNA synthetase alpha chain
    EC=6.1.1.20
Alternative name(s):
    Phenylalanine--tRNA ligase alpha chain
      Short name=PheRS

Gene names

Name:	pheS
Ordered Locus Names:	BU129

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (Acyrthosiphon pisum symbiotic bacterium) [Complete proteome] [HAMAP]

Taxonomic identifier

118099 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	329 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe). HAMAP MF_00281
Cofactor	Binds 2 magnesium ions per tetramer Potential. HAMAP MF_00281
Subunit structure	Tetramer of two alpha and two beta chains By similarity. HAMAP MF_00281
Subcellular location	Cytoplasm HAMAP MF_00281.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily.
Caution	Lacks the conserved glutamate residue that binds magnesium.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phenylalanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP phenylalanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP tRNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 329	329	Phenylalanyl-tRNA synthetase alpha chain HAMAP MF_00281		PRO_0000126676

Sequences

	Sequence		Length	Mass (Da)
	P57229-1 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: 27F0F0E5A5F7768E	FASTA	329	38,868
	10 20 30 40 50 60 MLNLNKLFEI IEIDIQNSKK ISELDKIRIK YLGKKGVLTA FMKKLKDFSY EDKKKHSIII 70 80 90 100 110 120 NKKKQEIISK INIKKQKLSV YILEQRIKSE TIDISLPGRR TEHGGFHPIT NTIDYIKNFF 130 140 150 160 170 180 LKLGFQSINS PEIEDEYHNF DALNISKYHP ARDSHDTFWF DRNRLLRTQT SSMQIRIMKQ 190 200 210 220 230 240 EKPPIRFIFP GKVYRNDYDI THTPMFHQIE GLIVDKNINF SNLKWIIYKF LYDFFGKEVL 250 260 270 280 290 300 IKFRPSYFPF TVPSAEVDII DHNGKSLEIL GCGMVHPNVL KNVNIDSKIY SACAFGLGIE 310 320 RIAMLRYGIT DIRSFFENDI RFLKQFKYN

References

[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed: 10993077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tokyo 1998.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000003 Genomic DNA. Translation: BAB12847.1.
RefSeq	NP_239961.1.
3D structure databases
SMR	P57229. Positions 83-328.
ModBase	Search...
Genome annotation databases
GeneID	1109573.
GenomeReviews	Gene locus BU129 in contig BA000003_GR.
KEGG	buc:BU129.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG284353.
OMA	FRASYFP.
PhylomeDB	P57229.
Enzyme and pathway databases
BioCyc	BSP107806:BU129-MONOMER.
Family and domain databases
HAMAP	MF_00281. Phe_tRNA_synth_alpha1. [Tree]
InterPro	IPR006195. aa-tRNA-synth_II_cons-dom. IPR004529. Phe-tRNA-synth_IIc_asu. IPR004188. Phe-tRNA_synth_II_N. IPR002319. Phenylalanyl-tRNA_Synthase_acu. IPR010978. tRNA_bd_arm. [Graphical view]
PANTHER	PTHR11538. tRNA-synt_2d. 1 hit.
Pfam	PF02912. Phe_tRNA-synt_N. 1 hit. PF01409. tRNA-synt_2d. 1 hit. [Graphical view]
TIGRFAMs	TIGR00468. pheS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYFA_BUCAI

Accession

Primary (citable) accession number: P57229

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	February 9, 2010
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)