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P57219 (END3_BUCAI) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 89. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Endonuclease III

EC=4.2.99.18
Alternative name(s):
DNA-(apurinic or apyrimidinic site) lyase
Gene names
Name:nth
Ordered Locus Names:BU119
OrganismBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium) [Reference proteome] [HAMAP]
Taxonomic identifier107806 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera

Protein attributes

Sequence length210 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

DNA repair enzyme that has both DNA N-glycosylase activity and AP-lyase activity. The DNA N-glycosylase activity releases various damaged pyrimidines from DNA by cleaving the N-glycosidic bond, leaving an AP (apurinic/apyrimidinic) site. The AP-lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate By similarity. HAMAP-Rule MF_00942

Catalytic activity

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate. HAMAP-Rule MF_00942

Cofactor

Binds 1 4Fe-4S cluster By similarity. HAMAP-Rule MF_00942

Sequence similarities

Belongs to the Nth/MutY family.

Contains 1 HhH domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 210210Endonuclease III HAMAP-Rule MF_00942
PRO_0000102214

Regions

Domain108 – 12720HhH

Sites

Metal binding1871Iron-sulfur (4Fe-4S) By similarity
Metal binding1941Iron-sulfur (4Fe-4S) By similarity
Metal binding1971Iron-sulfur (4Fe-4S) By similarity
Metal binding2031Iron-sulfur (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
P57219 [UniParc].

Last modified December 1, 2000. Version 1.
Checksum: C43A060F24694660

FASTA21024,388
        10         20         30         40         50         60 
MNKKKRYEIL SILSRNNPEP KIELFFSSDF ELLLSVILSA QSTDFIVNKT TKILFKIANT 

        70         80         90        100        110        120 
PETIFLLGLE RLKNYIKDIG LYNTKALNII RTSFIILTKY NSIVPNNRIE LESLPGVGRK 

       130        140        150        160        170        180 
TANIILNILF KKKTIAVDTH VFRVCNRTNF AKGKNVKIVE EKLIKVVPSI FKLNFHSWFI 

       190        200        210 
LHGRYICTAR KIKCNICLIF KLCEFKQKIF 

« Hide

References

[1]"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: APS.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000003 Genomic DNA. Translation: BAB12837.1.
RefSeqNP_239951.1. NC_002528.1.

3D structure databases

ProteinModelPortalP57219.
SMRP57219. Positions 1-209.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAB12837; BAB12837; BAB12837.
GeneID1109563.
KEGGbuc:BU119.
PATRIC21243752. VBIBucAph127364_0128.

Phylogenomic databases

eggNOGCOG0177.
HOGENOMHOG000252206.
KOK10773.
OMANNKSKHL.
OrthoDBEOG6H4KC5.
ProtClustDBCLSK315401.

Enzyme and pathway databases

BioCycBAPH107806:GBZJ-119-MONOMER.

Family and domain databases

Gene3D1.10.1670.10. 1 hit.
1.10.340.30. 1 hit.
HAMAPMF_00942. Nth.
InterProIPR011257. DNA_glycosylase.
IPR004036. Endonuclease-III-like_CS2.
IPR003651. Endouclease3_FeS-loop_motif.
IPR003265. HhH-GPD_domain.
IPR000445. HhH_motif.
IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR023170. HTH_base_excis_C.
IPR005759. Nth.
[Graphical view]
PfamPF00633. HHH. 1 hit.
PF00730. HhH-GPD. 1 hit.
[Graphical view]
PIRSFPIRSF001435. Nth. 1 hit.
SMARTSM00478. ENDO3c. 1 hit.
SM00525. FES. 1 hit.
SM00278. HhH1. 1 hit.
[Graphical view]
SUPFAMSSF48150. SSF48150. 1 hit.
TIGRFAMsTIGR01083. nth. 1 hit.
PROSITEPS01155. ENDONUCLEASE_III_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameEND3_BUCAI
AccessionPrimary (citable) accession number: P57219
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: February 19, 2014
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Buchnera aphidicola (subsp. Acyrthosiphon pisum)

Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names