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P57219

- END3_BUCAI

UniProt

P57219 - END3_BUCAI

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Protein

Endonuclease III

Gene

nth

Organism
Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

DNA repair enzyme that has both DNA N-glycosylase activity and AP-lyase activity. The DNA N-glycosylase activity releases various damaged pyrimidines from DNA by cleaving the N-glycosidic bond, leaving an AP (apurinic/apyrimidinic) site. The AP-lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.UniRule annotation

Catalytic activityi

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi187 – 1871Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi194 – 1941Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi197 – 1971Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi203 – 2031Iron-sulfur (4Fe-4S)UniRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. DNA-(apurinic or apyrimidinic site) lyase activity Source: UniProtKB-HAMAP
  3. DNA binding Source: UniProtKB-HAMAP
  4. DNA N-glycosylase activity Source: UniProtKB-HAMAP
  5. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. base-excision repair Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase, Lyase

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

4Fe-4S, DNA-binding, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciBAPH107806:GBZJ-119-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Endonuclease IIIUniRule annotation (EC:4.2.99.18UniRule annotation)
Alternative name(s):
DNA-(apurinic or apyrimidinic site) lyaseUniRule annotation
Gene namesi
Name:nthUniRule annotation
Ordered Locus Names:BU119
OrganismiBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Taxonomic identifieri107806 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
ProteomesiUP000001806: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 210210Endonuclease IIIPRO_0000102214Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP57219.
SMRiP57219. Positions 1-209.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini108 – 12720HhHUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the Nth/MutY family.UniRule annotation
Contains 1 HhH domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG0177.
HOGENOMiHOG000252206.
KOiK10773.
OMAiDTHIYRV.
OrthoDBiEOG6H4KC5.

Family and domain databases

Gene3Di1.10.1670.10. 1 hit.
1.10.340.30. 1 hit.
HAMAPiMF_00942. Nth.
InterProiIPR011257. DNA_glycosylase.
IPR004036. Endonuclease-III-like_CS2.
IPR003651. Endouclease3_FeS-loop_motif.
IPR003265. HhH-GPD_domain.
IPR000445. HhH_motif.
IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR023170. HTH_base_excis_C.
IPR005759. Nth.
[Graphical view]
PfamiPF00633. HHH. 1 hit.
PF00730. HhH-GPD. 1 hit.
[Graphical view]
PIRSFiPIRSF001435. Nth. 1 hit.
SMARTiSM00478. ENDO3c. 1 hit.
SM00525. FES. 1 hit.
SM00278. HhH1. 1 hit.
[Graphical view]
SUPFAMiSSF48150. SSF48150. 1 hit.
TIGRFAMsiTIGR01083. nth. 1 hit.
PROSITEiPS01155. ENDONUCLEASE_III_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P57219-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNKKKRYEIL SILSRNNPEP KIELFFSSDF ELLLSVILSA QSTDFIVNKT
60 70 80 90 100
TKILFKIANT PETIFLLGLE RLKNYIKDIG LYNTKALNII RTSFIILTKY
110 120 130 140 150
NSIVPNNRIE LESLPGVGRK TANIILNILF KKKTIAVDTH VFRVCNRTNF
160 170 180 190 200
AKGKNVKIVE EKLIKVVPSI FKLNFHSWFI LHGRYICTAR KIKCNICLIF
210
KLCEFKQKIF
Length:210
Mass (Da):24,388
Last modified:December 1, 2000 - v1
Checksum:iC43A060F24694660
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000003 Genomic DNA. Translation: BAB12837.1.
RefSeqiNP_239951.1. NC_002528.1.

Genome annotation databases

EnsemblBacteriaiBAB12837; BAB12837; BAB12837.
GeneIDi1109563.
KEGGibuc:BU119.
PATRICi21243752. VBIBucAph127364_0128.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000003 Genomic DNA. Translation: BAB12837.1 .
RefSeqi NP_239951.1. NC_002528.1.

3D structure databases

ProteinModelPortali P57219.
SMRi P57219. Positions 1-209.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAB12837 ; BAB12837 ; BAB12837 .
GeneIDi 1109563.
KEGGi buc:BU119.
PATRICi 21243752. VBIBucAph127364_0128.

Phylogenomic databases

eggNOGi COG0177.
HOGENOMi HOG000252206.
KOi K10773.
OMAi DTHIYRV.
OrthoDBi EOG6H4KC5.

Enzyme and pathway databases

BioCyci BAPH107806:GBZJ-119-MONOMER.

Family and domain databases

Gene3Di 1.10.1670.10. 1 hit.
1.10.340.30. 1 hit.
HAMAPi MF_00942. Nth.
InterProi IPR011257. DNA_glycosylase.
IPR004036. Endonuclease-III-like_CS2.
IPR003651. Endouclease3_FeS-loop_motif.
IPR003265. HhH-GPD_domain.
IPR000445. HhH_motif.
IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR023170. HTH_base_excis_C.
IPR005759. Nth.
[Graphical view ]
Pfami PF00633. HHH. 1 hit.
PF00730. HhH-GPD. 1 hit.
[Graphical view ]
PIRSFi PIRSF001435. Nth. 1 hit.
SMARTi SM00478. ENDO3c. 1 hit.
SM00525. FES. 1 hit.
SM00278. HhH1. 1 hit.
[Graphical view ]
SUPFAMi SSF48150. SSF48150. 1 hit.
TIGRFAMsi TIGR01083. nth. 1 hit.
PROSITEi PS01155. ENDONUCLEASE_III_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
    Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
    Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: APS.

Entry informationi

Entry nameiEND3_BUCAI
AccessioniPrimary (citable) accession number: P57219
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: November 26, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Buchnera aphidicola (subsp. Acyrthosiphon pisum)
    Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3