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Protein

Electron transport complex subunit B

Gene

BU114

Organism
Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Part of a membrane complex involved in electron transport.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [2Fe-2S] clusterUniRule annotationNote: Binds 1 [2Fe-2S] cluster.UniRule annotation
  • [4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi45 – 451Iron-sulfur 1 (2Fe-2S)UniRule annotation
Metal bindingi48 – 481Iron-sulfur 1 (2Fe-2S)UniRule annotation
Metal bindingi53 – 531Iron-sulfur 1 (2Fe-2S)UniRule annotation
Metal bindingi70 – 701Iron-sulfur 1 (2Fe-2S)UniRule annotation
Metal bindingi113 – 1131Iron-sulfur 2 (4Fe-4S)UniRule annotation
Metal bindingi116 – 1161Iron-sulfur 2 (4Fe-4S)UniRule annotation
Metal bindingi119 – 1191Iron-sulfur 2 (4Fe-4S)UniRule annotation
Metal bindingi123 – 1231Iron-sulfur 3 (4Fe-4S)UniRule annotation
Metal bindingi143 – 1431Iron-sulfur 3 (4Fe-4S)UniRule annotation
Metal bindingi146 – 1461Iron-sulfur 3 (4Fe-4S)UniRule annotation
Metal bindingi149 – 1491Iron-sulfur 3 (4Fe-4S)UniRule annotation
Metal bindingi153 – 1531Iron-sulfur 2 (4Fe-4S)UniRule annotation

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. electron carrier activity Source: InterPro
  4. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. electron transport chain Source: InterPro
Complete GO annotation...

Keywords - Biological processi

Electron transport, Transport

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciBAPH107806:GBZJ-114-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Electron transport complex subunit BUniRule annotation
Gene namesi
Ordered Locus Names:BU114
OrganismiBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Taxonomic identifieri107806 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
ProteomesiUP000001806: Chromosome

Subcellular locationi

Cell inner membrane UniRule annotation; Peripheral membrane protein UniRule annotation

GO - Cellular componenti

  1. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 167167Electron transport complex subunit BPRO_0000216268Add
BLAST

Interactioni

Subunit structurei

Composed of six subunits.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliP57214.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini28 – 87604Fe-4SAdd
BLAST
Domaini104 – 133304Fe-4S ferredoxin-type 1UniRule annotationAdd
BLAST
Domaini134 – 163304Fe-4S ferredoxin-type 2UniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 2222HydrophobicAdd
BLAST

Sequence similaritiesi

Belongs to the 4Fe4S bacterial-type ferredoxin family. RnfB subfamily.UniRule annotation
Contains 1 4Fe-4S domain.Curated
Contains 2 4Fe-4S ferredoxin-type domains.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG2878.
HOGENOMiHOG000262938.
KOiK03616.
OMAiKVEGDPI.
OrthoDBiEOG60SCM3.

Family and domain databases

HAMAPiMF_00463. RsxB_RnfB.
InterProiIPR007202. 4Fe-4S_dom.
IPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR010207. Elect_transpt_cplx_RnfB.
IPR016463. RnfB_Proteobac.
[Graphical view]
PfamiPF04060. FeS. 1 hit.
[Graphical view]
PIRSFiPIRSF005784. Elect_transpt_RnfB. 1 hit.
TIGRFAMsiTIGR01944. rnfB. 1 hit.
PROSITEiPS51656. 4FE4S. 1 hit.
PS00198. 4FE4S_FER_1. 2 hits.
PS51379. 4FE4S_FER_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P57214-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MITLIIFSFL SFLLGIILSF TAYKFRSQED PIVAIVNELL PQSQCAQCGY
60 70 80 90 100
SGCYPYAKAI VENSEKINKC IPGGTDLISA ISSVLSIEVP EKNLIITHKK
110 120 130 140 150
QKNNTVLINE SNCVGCSKCA SFCPVDAIVG APNFIHTVLQ EFCTGCNICL
160
LHCPTNCIEI KKETYEE
Length:167
Mass (Da):18,333
Last modified:March 1, 2001 - v1
Checksum:iD9DC969A3C3868A1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000003 Genomic DNA. Translation: BAB12832.1.
RefSeqiNP_239946.1. NC_002528.1.

Genome annotation databases

EnsemblBacteriaiBAB12832; BAB12832; BAB12832.
GeneIDi1109558.
KEGGibuc:BU114.
PATRICi21243738. VBIBucAph127364_0121.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000003 Genomic DNA. Translation: BAB12832.1.
RefSeqiNP_239946.1. NC_002528.1.

3D structure databases

ProteinModelPortaliP57214.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB12832; BAB12832; BAB12832.
GeneIDi1109558.
KEGGibuc:BU114.
PATRICi21243738. VBIBucAph127364_0121.

Phylogenomic databases

eggNOGiCOG2878.
HOGENOMiHOG000262938.
KOiK03616.
OMAiKVEGDPI.
OrthoDBiEOG60SCM3.

Enzyme and pathway databases

BioCyciBAPH107806:GBZJ-114-MONOMER.

Family and domain databases

HAMAPiMF_00463. RsxB_RnfB.
InterProiIPR007202. 4Fe-4S_dom.
IPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR010207. Elect_transpt_cplx_RnfB.
IPR016463. RnfB_Proteobac.
[Graphical view]
PfamiPF04060. FeS. 1 hit.
[Graphical view]
PIRSFiPIRSF005784. Elect_transpt_RnfB. 1 hit.
TIGRFAMsiTIGR01944. rnfB. 1 hit.
PROSITEiPS51656. 4FE4S. 1 hit.
PS00198. 4FE4S_FER_1. 2 hits.
PS51379. 4FE4S_FER_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
    Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
    Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: APS.

Entry informationi

Entry nameiETCB_BUCAI
AccessioniPrimary (citable) accession number: P57214
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 24, 2001
Last sequence update: March 1, 2001
Last modified: January 7, 2015
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Buchnera aphidicola (subsp. Acyrthosiphon pisum)
    Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.