P57214 (RNFB_BUCAI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Electron transport complex protein RnfB

Gene names

Name:	rnfB
Ordered Locus Names:	BU114

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium) [Reference proteome] [HAMAP]

Taxonomic identifier

107806 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera › Buchnera aphidicola › Buchnera aphidicola (Acyrthosiphon pisum)

Protein attributes

Sequence length	167 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	May be part of a membrane complex involved in electron transport By similarity. HAMAP-Rule MF_00463
Cofactor	Binds 1 2Fe-2S cluster Potential. Binds 2 4Fe-4S clusters Potential.
Subunit structure	Composed of at least six subunits; RnfA, RnfB, RnfC, RnfD, RnfE and RnfG By similarity.
Subcellular location	Cytoplasm. Cell membrane; Peripheral membrane protein By similarity HAMAP-Rule MF_00463.
Sequence similarities	Belongs to the 4Fe4S bacterial-type ferredoxin family. RnfB subfamily. Contains 1 4Fe-4S domain. Contains 2 4Fe-4S ferredoxin-type domains.

Ontologies

Keywords
Biological process	Electron transport Transport
Cellular component	Cell membrane Cytoplasm Membrane
Domain	Repeat
Ligand	2Fe-2S 4Fe-4S Iron Iron-sulfur Metal-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW nitrogen fixation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 167	167	Electron transport complex protein RnfB HAMAP-Rule MF_00463		PRO_0000216268
Regions
	Domain	28 – 87	60	4Fe-4S
	Domain	104 – 133	30	4Fe-4S ferredoxin-type 1
	Domain	134 – 163	30	4Fe-4S ferredoxin-type 2
Sites
	Metal binding	45	1	Iron-sulfur 1 (2Fe-2S) Potential
	Metal binding	48	1	Iron-sulfur 1 (2Fe-2S) Potential
	Metal binding	53	1	Iron-sulfur 1 (2Fe-2S) Potential
	Metal binding	70	1	Iron-sulfur 1 (2Fe-2S) Potential
	Metal binding	113	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	116	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	119	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	123	1	Iron-sulfur 3 (4Fe-4S) By similarity
	Metal binding	143	1	Iron-sulfur 3 (4Fe-4S) By similarity
	Metal binding	146	1	Iron-sulfur 3 (4Fe-4S) By similarity
	Metal binding	149	1	Iron-sulfur 3 (4Fe-4S) By similarity
	Metal binding	153	1	Iron-sulfur 2 (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	P57214 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: D9DC969A3C3868A1	FASTA	167	18,333
	10 20 30 40 50 60 MITLIIFSFL SFLLGIILSF TAYKFRSQED PIVAIVNELL PQSQCAQCGY SGCYPYAKAI 70 80 90 100 110 120 VENSEKINKC IPGGTDLISA ISSVLSIEVP EKNLIITHKK QKNNTVLINE SNCVGCSKCA 130 140 150 160 SFCPVDAIVG APNFIHTVLQ EFCTGCNICL LHCPTNCIEI KKETYEE

References

[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: APS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000003 Genomic DNA. Translation: BAB12832.1.
RefSeq	NP_239946.1. NC_002528.1.
3D structure databases
ProteinModelPortal	P57214.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAB12832; BAB12832; BAB12832.
GeneID	1109558.
KEGG	buc:BU114.
PATRIC	21243738. VBIBucAph127364_0121.
Phylogenomic databases
eggNOG	COG2878.
HOGENOM	HOG000262938.
KO	K03616.
OMA	NGEMINK.
ProtClustDB	CLSK390906.
Family and domain databases
HAMAP	MF_00463. RnfB.
InterPro	IPR007202. 4Fe-4S_dom. IPR001450. 4Fe4S-bd_dom. IPR017896. 4Fe4S_Fe-S-bd. IPR017900. 4Fe4S_Fe_S_CS. IPR010207. Elect_transpt_cplx_RnfB. IPR016463. RnfB_Proteobac. [Graphical view]
Pfam	PF00037. Fer4. 2 hits. PF04060. FeS. 1 hit. [Graphical view]
PIRSF	PIRSF005784. Elect_transpt_RnfB. 1 hit.
TIGRFAMs	TIGR01944. rnfB. 1 hit.
PROSITE	PS51656. 4FE4S. 1 hit. PS00198. 4FE4S_FER_1. 2 hits. PS51379. 4FE4S_FER_2. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNFB_BUCAI

Accession

Primary (citable) accession number: P57214

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 24, 2001
Last sequence update:	March 1, 2001
Last modified:	May 1, 2013
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program