P57212 (RISA_BUCAI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Riboflavin synthase
Short name=RS
EC=2.5.1.9

Gene names

Name:	ribE
Ordered Locus Names:	BU112

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium) [Reference proteome] [HAMAP]

Taxonomic identifier

107806 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera › Buchnera aphidicola › Buchnera aphidicola (Acyrthosiphon pisum)

Protein attributes

Sequence length	208 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the dismutation of two molecules of 6,7-dimethyl-8-ribityllumazine, resulting in the formation of riboflavin and 5-amino-6-(D-ribitylamino)uracil By similarity.
Catalytic activity	2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine.
Pathway	Cofactor biosynthesis; riboflavin biosynthesis; riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil: step 2/2.
Subunit structure	Homotrimer By similarity.
Sequence similarities	Contains 2 lumazine-binding repeats.

Ontologies

Keywords
Biological process	Riboflavin biosynthesis
Domain	Repeat
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	riboflavin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	oxidoreductase activity Inferred from electronic annotation. Source: InterPro riboflavin synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 208	208	Riboflavin synthase		PRO_0000068160
Regions
	Repeat	1 – 97	97	Lumazine-binding 1
	Repeat	98 – 195	98	Lumazine-binding 2
	Region	4 – 6	3	Substrate binding By similarity
	Region	48 – 50	3	Substrate binding By similarity
	Region	62 – 67	6	Substrate binding By similarity

Sequences

	Sequence		Length	Mass (Da)
	P57212 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: 7CCE3272CF54BA5B	FASTA	208	23,505
	10 20 30 40 50 60 MFTGIINGTA TIVYIEKKKK KYRYTVELPS NLSKNLKLGD SISHNGCCLT VKLINNSFII 70 80 90 100 110 120 CDVIQETLKS TNLGILNIGD SINIERSIKY GDEIGGHIIS GHVMNTAEIS KMSKSDNNCV 130 140 150 160 170 180 IWLKMNNMSL MKYIFYKGFI CVDGISLTVN DIIKNEFCVN IIPYTFLFTT IKDKKNGSLV 190 200 NIEIDFYTQT IVDTTERLIN NNLKSLYD

References

[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: APS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000003 Genomic DNA. Translation: BAB12830.1.
RefSeq	NP_239944.1. NC_002528.1.
3D structure databases
ProteinModelPortal	P57212.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAB12830; BAB12830; BAB12830.
GeneID	1109556.
KEGG	buc:BU112.
PATRIC	21243734. VBIBucAph127364_0119.
Phylogenomic databases
eggNOG	COG0307.
HOGENOM	HOG000151758.
KO	K00793.
OMA	HILSGHV.
ProtClustDB	PRK09289.
Enzyme and pathway databases
BioCyc	BAPH107806:GBZJ-112-MONOMER.
UniPathway	UPA00275; UER00405.
Family and domain databases
Gene3D	2.40.30.20. 2 hits.
InterPro	IPR023366. ATPase_F1/A1-cplx_a_su_N. IPR001783. Lumazine-bd. IPR026017. Lumazine-bd_dom. IPR017938. Riboflavin_synthase-like_b-brl. [Graphical view]
PANTHER	PTHR21098. PTHR21098. 1 hit.
Pfam	PF00677. Lum_binding. 2 hits. [Graphical view]
PIRSF	PIRSF000498. Riboflavin_syn_A. 1 hit.
SUPFAM	SSF63380. Riboflavin_synthase_like_b-brl. 2 hits.
TIGRFAMs	TIGR00187. ribE. 1 hit.
PROSITE	PS51177. LUMAZINE_BIND. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RISA_BUCAI

Accession

Primary (citable) accession number: P57212

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	May 29, 2013
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program