Reviewed, UniProtKB/Swiss-Prot P57212 (RISA_BUCAI)

Names and origin

Protein names

Recommended name:
    Riboflavin synthase alpha chain
    EC=2.5.1.9

Gene names

Name:	ribE
Ordered Locus Names:	BU112

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (Acyrthosiphon pisum symbiotic bacterium) [Complete proteome] [HAMAP]

Taxonomic identifier

118099 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	208 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)-ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2-butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The alpha subunit catalyzes the dismutation of 6,7-dimethyl-8-lumazine to riboflavin and 5-amino-6-(1'-D)-ribityl-amino-2,4(1H,3H)-pyrimidinedione By similarity.
Catalytic activity	2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine.
Pathway	Cofactor biosynthesis; riboflavin biosynthesis; 5-amino-6-(D-ribitylamino)uracil and riboflavin from 6,7-dimethyl-8-(1-D-ribityl)lumazine: step 1/1.
Subunit structure	Oligomer that consist of 3 alpha subunits and 60 beta subunits By similarity.
Sequence similarities	Contains 2 lumazine-binding repeats.

Ontologies

Keywords
Biological process	Riboflavin biosynthesis
Domain	Repeat
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	riboflavin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	riboflavin synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 208	208	Riboflavin synthase alpha chain		PRO_0000068160
Regions
	Repeat	1 – 97	97	Lumazine-binding 1
	Repeat	98 – 195	98	Lumazine-binding 2

Sequences

	Sequence		Length	Mass (Da)
	P57212-1 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: 7CCE3272CF54BA5B	FASTA	208	23,505
	10 20 30 40 50 60 MFTGIINGTA TIVYIEKKKK KYRYTVELPS NLSKNLKLGD SISHNGCCLT VKLINNSFII 70 80 90 100 110 120 CDVIQETLKS TNLGILNIGD SINIERSIKY GDEIGGHIIS GHVMNTAEIS KMSKSDNNCV 130 140 150 160 170 180 IWLKMNNMSL MKYIFYKGFI CVDGISLTVN DIIKNEFCVN IIPYTFLFTT IKDKKNGSLV 190 200 NIEIDFYTQT IVDTTERLIN NNLKSLYD

References

[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed: 10993077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tokyo 1998.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000003 Genomic DNA. Translation: BAB12830.1.
RefSeq	NP_239944.1.
3D structure databases
HSSP	HSSP built from PDB template 1I8D based on UniProtKB P29015.
ModBase	Search...
Genome annotation databases
GeneID	1109556.
GenomeReviews	Gene locus BU112 in contig BA000003_GR.
KEGG	buc:BU112.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P57212.
OMA	CLTVTKI.
Enzyme and pathway databases
BioCyc	BSP107806:BU112-MON.
Family and domain databases
InterPro	IPR001783. Lumazine_bd. [Graphical view]
PANTHER	PTHR21098. Lumazine_bd. 1 hit.
Pfam	PF00677. Lum_binding. 2 hits. [Graphical view]
PIRSF	PIRSF000498. Riboflavin_syn_A. 1 hit.
ProDom	PD004110. Lum_binding. 2 hits. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00187. ribE. 1 hit.
PROSITE	PS51177. LUMAZINE_BIND. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RISA_BUCAI

Accession

Primary (citable) accession number: P57212

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	November 3, 2009
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)