Reviewed, UniProtKB/Swiss-Prot P57210 (SYM_BUCAI)

Names and origin

Protein names

Recommended name:
    Methionyl-tRNA synthetase
    EC=6.1.1.10
Alternative name(s):
    Methionine--tRNA ligase
      Short name=MetRS

Gene names

Name:	metG
Ordered Locus Names:	BU109

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (Acyrthosiphon pisum symbiotic bacterium) [Complete proteome] [HAMAP]

Taxonomic identifier

118099 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	547 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation By similarity.
Catalytic activity	ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met). HAMAP MF_00098
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm. HAMAP MF_00098
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP methionine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 547	547	Methionyl-tRNA synthetase HAMAP MF_00098		PRO_0000139111
Regions
	Motif	15 – 25	11	"HIGH" region HAMAP MF_00098
	Motif	332 – 336	5	"KMSKS" region HAMAP MF_00098
Sites
	Metal binding	146	1	Zinc By similarity
	Metal binding	149	1	Zinc By similarity
	Metal binding	159	1	Zinc By similarity
	Metal binding	162	1	Zinc By similarity
	Binding site	335	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P57210-1 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: FFEF802670A15EB7	FASTA	547	64,283
	10 20 30 40 50 60 MSSVFRKILV TCALPYANGS IHIGHMLEHI QADIWVRYHR MRGHEVWFVS ADDAHGTAIM 70 80 90 100 110 120 LKAQDLEISP NKLIKNIRIE HQIDFSNFKI SHDNYYSTHS LENLYLSRKI FTCLNEKGLI 130 140 150 160 170 180 KEKKIFQLYD TVKKIFLPDR FIKGTCPICK SKNQYGDNCE ICSATYEPTD LINPISVISG 190 200 210 220 230 240 KKPILKNTKH LYFDLPSFTN MLKKWIHSGV LEESVIKKTE EWFKVGLKSW AISRDAPYFG 250 260 270 280 290 300 FKIPNYPNKY FYVWLDAPIG YISAFKNLCF KSKKLNFNEL WNQNSNYELY HFIGKDIIYF 310 320 330 340 350 360 HTLFWPAILE AVSFRQPSGI FVHGHLTMNG LKLSKSRGAL IKASDWIQYF DSDSLRYYYA 370 380 390 400 410 420 SKLSNKTHDI EINLEDFIQK INSDIVNKLV NLAARNASFI NKYFNGYLSD KLSNIKLYKY 430 440 450 460 470 480 FVNTSSSIED FFENREFSFI VKESMRLLDV ANQYINEKKP WKIKRTEENI RELQNICTMG 490 500 510 520 530 540 INLFRIIMIF LKPIVPDLAI KTESFLISKL TWDGIKKPLL SHQINKFFPL YKRIDVEKMF EFMNICR

References

[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed: 10993077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tokyo 1998.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000003 Genomic DNA. Translation: BAB12828.1.
RefSeq	NP_239942.1.
3D structure databases
HSSP	HSSP built from PDB template 1MEA based on UniProtKB P00959.
ModBase	Search...
Genome annotation databases
GeneID	1109554.
GenomeReviews	Gene locus BU109 in contig BA000003_GR.
KEGG	buc:BU109.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P57210.
OMA	DFSWREF.
Enzyme and pathway databases
BioCyc	BSP107806:BU109-MON.
Family and domain databases
HAMAP	MF_00098. [Tree]
InterPro	IPR015413. aa-tRNA-synt_I. IPR001412. aa-tRNA-synth_I_CS. IPR002304. Met-tRNA-synth_Ia. IPR014729. Rossmann-like_a/b/a_fold. IPR014758. tRNA-synt_met_N. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF09334. tRNA-synt_1g. 1 hit. [Graphical view]
PRINTS	PR01041. TRNASYNTHMET.
TIGRFAMs	TIGR00398. metG. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYM_BUCAI

Accession

Primary (citable) accession number: P57210

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	November 3, 2009
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)