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P57208 (6PGD_BUCAI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
6-phosphogluconate dehydrogenase, decarboxylating

EC=1.1.1.44
Gene names
Name:gnd
Ordered Locus Names:BU107
OrganismBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium) [Reference proteome] [HAMAP]
Taxonomic identifier107806 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera

Protein attributes

Sequence length468 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the oxidative decarboxylation of 6-phosphogluconate to ribulose 5-phosphate and CO2, with concomitant reduction of NADP to NADPH By similarity.

Catalytic activity

6-phospho-D-gluconate + NADP+ = D-ribulose 5-phosphate + CO2 + NADPH.

Pathway

Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 3/3.

Subunit structure

Homodimer By similarity.

Sequence similarities

Belongs to the 6-phosphogluconate dehydrogenase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 4684686-phosphogluconate dehydrogenase, decarboxylating
PRO_0000090028

Regions

Nucleotide binding10 – 156NADP By similarity
Nucleotide binding33 – 353NADP By similarity
Nucleotide binding74 – 763NADP By similarity
Region128 – 1303Substrate binding By similarity
Region185 – 1862Substrate binding By similarity

Sites

Active site1821Proton acceptor By similarity
Active site1891Proton donor By similarity
Binding site1021NADP By similarity
Binding site1021Substrate By similarity
Binding site1901Substrate By similarity
Binding site2591Substrate; via amide nitrogen By similarity
Binding site2861Substrate By similarity
Binding site4451Substrate; shared with dimeric partner By similarity
Binding site4511Substrate; shared with dimeric partner By similarity

Sequences

Sequence LengthMass (Da)Tools
P57208 [UniParc].

Last modified December 1, 2000. Version 1.
Checksum: 3317FDDF8F3EF20A

FASTA46853,574
        10         20         30         40         50         60 
MSRQQIGVVG MAVMGRNLAL NIESKNYSVS IFNRTRSVTE EVFNQNKKKN IVPYFSIKDF 

        70         80         90        100        110        120 
IDSLLKPRCI LLMVQSGKAT DETIKMILPY LEKEDILIDA GNTFYKDTIR RNEKLSKYEI 

       130        140        150        160        170        180 
NFIGMGVSGG ELGALNGPSI MPGGQKEAYK LVLPMLEKIS AKFKGEPCVS YIGPNGAGHY 

       190        200        210        220        230        240 
VKMVHNGIEY GDMQLISESY FLLKYLLNMS NEELSSTFSK WNKGELNSYL IEITKNIFIE 

       250        260        270        280        290        300 
KDEKGKYLID RILDVAEDKG TGKWISKSAL DLREPLSLIT ESVFARYLSS LKRQRIIASK 

       310        320        330        340        350        360 
ILQGPKIKTF IKDKNSFIEE VRRALYLGKI ISYAQGFSQL KRASEKYHWN LKYGEIAKIF 

       370        380        390        400        410        420 
RAGCIIRANF LQKITDEYTQ NKNVVNLLLT PYFSKIANEY ENSLRNIVMY AIKYGISTPT 

       430        440        450        460 
FSAAISYYDS YRALYLPANL IQAQRDYFGS HTYQRTDQTG YFHTNWSQ 

« Hide

References

[1]"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: APS.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000003 Genomic DNA. Translation: BAB12826.1.
RefSeqNP_239940.1. NC_002528.1.

3D structure databases

ProteinModelPortalP57208.
SMRP57208. Positions 1-466.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEP57208.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAB12826; BAB12826; BAB12826.
GeneID1109552.
KEGGbuc:BU107.
PATRIC21243724. VBIBucAph127364_0115.

Phylogenomic databases

eggNOGCOG0362.
HOGENOMHOG000255147.
KOK00033.
OMADKDGVFH.
OrthoDBEOG6MSS4W.

Enzyme and pathway databases

BioCycBAPH107806:GBZJ-107-MONOMER.
UniPathwayUPA00115; UER00410.

Family and domain databases

Gene3D1.10.1040.10. 1 hit.
1.20.5.320. 1 hit.
3.40.50.720. 1 hit.
InterProIPR008927. 6-PGluconate_DH_C-like.
IPR006114. 6PGDH_C.
IPR006113. 6PGDH_decarbox.
IPR006115. 6PGDH_NADP-bd.
IPR006184. 6PGdom_BS.
IPR013328. DH_multihelical.
IPR012284. Fibritin/6PGD_C-extension.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamPF00393. 6PGD. 1 hit.
PF03446. NAD_binding_2. 1 hit.
[Graphical view]
PIRSFPIRSF000109. 6PGD. 1 hit.
SUPFAMSSF48179. SSF48179. 1 hit.
TIGRFAMsTIGR00873. gnd. 1 hit.
PROSITEPS00461. 6PGD. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry name6PGD_BUCAI
AccessionPrimary (citable) accession number: P57208
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: May 14, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Buchnera aphidicola (subsp. Acyrthosiphon pisum)

Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names