P57208 (6PGD_BUCAI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
6-phosphogluconate dehydrogenase, decarboxylating
EC=1.1.1.44

Gene names

Name:	gnd
Ordered Locus Names:	BU107

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)

Taxonomic identifier

107806 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	468 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the oxidative decarboxylation of 6-phosphogluconate to ribulose 5-phosphate and CO2, with concomitant reduction of NADP to NADPH By similarity.
Catalytic activity	6-phospho-D-gluconate + NADP+ = D-ribulose 5-phosphate + CO2 + NADPH.
Pathway	Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 3/3.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the 6-phosphogluconate dehydrogenase family.

Ontologies

Keywords
Biological process	Gluconate utilization Pentose shunt
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	D-gluconate metabolic process Inferred from electronic annotation. Source: UniProtKB-KW pentose-phosphate shunt Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro phosphogluconate dehydrogenase (decarboxylating) activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 468	468	6-phosphogluconate dehydrogenase, decarboxylating		PRO_0000090028
Regions
	Nucleotide binding	10 – 15	6	NADP By similarity
	Nucleotide binding	33 – 35	3	NADP By similarity
	Nucleotide binding	74 – 76	3	NADP By similarity
	Region	128 – 130	3	Substrate binding By similarity
	Region	185 – 186	2	Substrate binding By similarity
Sites
	Active site	182	1	Proton acceptor By similarity
	Active site	189	1	Proton donor By similarity
	Binding site	102	1	NADP By similarity
	Binding site	102	1	Substrate By similarity
	Binding site	190	1	Substrate By similarity
	Binding site	259	1	Substrate; via amide nitrogen By similarity
	Binding site	286	1	Substrate By similarity
	Binding site	445	1	Substrate; shared with dimeric partner By similarity
	Binding site	451	1	Substrate; shared with dimeric partner By similarity

Sequences

	Sequence		Length	Mass (Da)
	P57208 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: 3317FDDF8F3EF20A	FASTA	468	53,574
	10 20 30 40 50 60 MSRQQIGVVG MAVMGRNLAL NIESKNYSVS IFNRTRSVTE EVFNQNKKKN IVPYFSIKDF 70 80 90 100 110 120 IDSLLKPRCI LLMVQSGKAT DETIKMILPY LEKEDILIDA GNTFYKDTIR RNEKLSKYEI 130 140 150 160 170 180 NFIGMGVSGG ELGALNGPSI MPGGQKEAYK LVLPMLEKIS AKFKGEPCVS YIGPNGAGHY 190 200 210 220 230 240 VKMVHNGIEY GDMQLISESY FLLKYLLNMS NEELSSTFSK WNKGELNSYL IEITKNIFIE 250 260 270 280 290 300 KDEKGKYLID RILDVAEDKG TGKWISKSAL DLREPLSLIT ESVFARYLSS LKRQRIIASK 310 320 330 340 350 360 ILQGPKIKTF IKDKNSFIEE VRRALYLGKI ISYAQGFSQL KRASEKYHWN LKYGEIAKIF 370 380 390 400 410 420 RAGCIIRANF LQKITDEYTQ NKNVVNLLLT PYFSKIANEY ENSLRNIVMY AIKYGISTPT 430 440 450 460 FSAAISYYDS YRALYLPANL IQAQRDYFGS HTYQRTDQTG YFHTNWSQ

References

[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed: 10993077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: APS.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000003 Genomic DNA. Translation: BAB12826.1.
RefSeq	NP_239940.1. NC_002528.1.
3D structure databases
ProteinModelPortal	P57208.
SMR	P57208. Positions 1-466.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBUCT00000002831; EBBUCP00000002607; EBBUCG00000002831.
GeneID	1109552.
GenomeReviews	Gene locus BU107 in contig BA000003_GR.
KEGG	buc:BU107.
PATRIC	21243724. VBIBucAph127364_0115.
Phylogenomic databases
GeneTree	EBGT00050000007968.
HOGENOM	HBG286913.
OMA	SNYPDTN.
PhylomeDB	P57208.
ProtClustDB	PRK09287.
Enzyme and pathway databases
BioCyc	BSP107806:BU107-MONOMER.
Family and domain databases
InterPro	IPR008927. 6-PGluconate_DH_C-like. IPR006114. 6PGDH_C. IPR006113. 6PGDH_decarbox. IPR006115. 6PGDH_NADP-bd. IPR006184. 6PGdom_BS. IPR013328. DH_multihelical. IPR012284. Fibritin/6PGD_C-extension. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:1.20.5.320. Fibritin/6PGD_C-extension. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. G3DSA:1.10.1040.10. Opine_DH. 1 hit.
KO	K00033.
Pfam	PF00393. 6PGD. 1 hit. PF03446. NAD_binding_2. 1 hit. [Graphical view]
PIRSF	PIRSF000109. 6PGD. 1 hit.
SUPFAM	SSF48179. 6DGDH_C_like. 1 hit.
TIGRFAMs	TIGR00873. Gnd. 1 hit.
PROSITE	PS00461. 6PGD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

6PGD_BUCAI

Accession

Primary (citable) accession number: P57208

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	January 25, 2012
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program