P57206 (HIS6_BUCAI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Imidazole glycerol phosphate synthase subunit HisF
EC=4.1.3.-

Alternative name(s):
IGP synthase cyclase subunit
IGP synthase subunit HisF
ImGP synthase subunit HisF
Short name=IGPS subunit HisF

Gene names

Name:	hisF
Ordered Locus Names:	BU105

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)

Taxonomic identifier

107806 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	258 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The HisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the HisH subunit By similarity. HAMAP MF_01013
Catalytic activity	5-[(5-phospho-1-deoxyribulos-1-ylamino)methylideneamino]-1-(5-phosphoribosyl)imidazole-4-carboxamide + L-glutamine = imidazole-glycerol phosphate + 5-aminoimidazol-4-carboxamide ribonucleotide + L-glutamate + H2O. HAMAP MF_01013
Pathway	Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 5/9. HAMAP MF_01013
Subunit structure	Heterodimer of HisH and HisF By similarity. HAMAP MF_01013
Subcellular location	Cytoplasm By similarity HAMAP MF_01013.
Sequence similarities	Belongs to the HisA/HisF family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Histidine biosynthesis
Cellular component	Cytoplasm
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	histidine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	imidazoleglycerol-phosphate synthase activity Inferred from electronic annotation. Source: InterPro lyase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 258	258	Imidazole glycerol phosphate synthase subunit HisF HAMAP MF_01013		PRO_0000142132
Sites
	Active site	11	1	Potential
	Active site	130	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	P57206 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: E4E86B0BB5BA0E4A	FASTA	258	28,621
	10 20 30 40 50 60 MLAKRIIACL DVDDGVVVKG IKFQNHKIVG DIVPLARRYA KEGIDELVFY DITAATKNKL 70 80 90 100 110 120 VDRSWIKNVS KVINIPFCVA GGIKSVEDAK KVLSSGADKI SINSSALIDP NLITKISERF 130 140 150 160 170 180 GVQCVVIGID SWFDKTKNSY MVQQYTGDIS KTYQTSWKTS DWVKKVQEKG AGEIVLNMMN 190 200 210 220 230 240 KDGLQKGYDI SHLSEIRKIC KVPLIASGGA GSVEHFYEAL YYSNVDGVLA ASVFHKNIVE 250 IKVLKKKLIA RGMEIREC

References

[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed: 10993077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: APS.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000003 Genomic DNA. Translation: BAB12824.1.
RefSeq	NP_239938.1. NC_002528.1.
3D structure databases
ProteinModelPortal	P57206.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBUCT00000002944; EBBUCP00000002720; EBBUCG00000002944.
GeneID	1109508.
GenomeReviews	Gene locus BU105 in contig BA000003_GR.
KEGG	buc:BU105.
PATRIC	21243720. VBIBucAph127364_0113.
Phylogenomic databases
GeneTree	EBGT00050000007994.
HOGENOM	HBG541613.
OMA	ASVFHKG.
PhylomeDB	P57206.
ProtClustDB	PRK02083.
Enzyme and pathway databases
BioCyc	BSP107806:BU105-MONOMER.
Family and domain databases
HAMAP	MF_01013. HisF. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR006062. His_biosynth. IPR004651. HisF. IPR011060. RibuloseP-bd_barrel. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K02500.
Pfam	PF00977. His_biosynth. 1 hit. [Graphical view]
SUPFAM	SSF51366. RibP_bind_barrel. 1 hit.
TIGRFAMs	TIGR00735. HisF. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HIS6_BUCAI

Accession

Primary (citable) accession number: P57206

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	January 25, 2012
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program