P57204 (HIS5_BUCAI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Imidazole glycerol phosphate synthase subunit HisH
EC=2.4.2.-

Alternative name(s):
IGP synthase glutamine amidotransferase subunit
IGP synthase subunit HisH
ImGP synthase subunit HisH
Short name=IGPS subunit HisH

Gene names

Name:	hisH
Ordered Locus Names:	BU103

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)

Taxonomic identifier

107806 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	196 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The HisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to HisF for the synthesis of IGP and AICAR By similarity. HAMAP MF_00278
Catalytic activity	5-[(5-phospho-1-deoxyribulos-1-ylamino)methylideneamino]-1-(5-phosphoribosyl)imidazole-4-carboxamide + L-glutamine = imidazole-glycerol phosphate + 5-aminoimidazol-4-carboxamide ribonucleotide + L-glutamate + H2O. HAMAP MF_00278
Pathway	Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 5/9. HAMAP MF_00278
Subunit structure	Heterodimer of HisH and HisF By similarity. HAMAP MF_00278
Subcellular location	Cytoplasm By similarity HAMAP MF_00278.
Sequence similarities	Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Histidine biosynthesis
Cellular component	Cytoplasm
Domain	Glutamine amidotransferase
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW histidine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	transferase activity, transferring pentosyl groups Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 196	196	Imidazole glycerol phosphate synthase subunit HisH HAMAP MF_00278		PRO_0000152354
Regions
	Domain	2 – 196	195	Glutamine amidotransferase type-1
Sites
	Active site	77	1	Nucleophile By similarity
	Active site	178	1	By similarity
	Active site	180	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P57204 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: 904624B74BBEBE5D	FASTA	196	21,679
	10 20 30 40 50 60 MYIVIVDTGC ANLTSIKVAI NNLGYNATIT SESSIILKSN KIFLPGVGTA SAVMDLLSKK 70 80 90 100 110 120 KIINVIRECK QTILGICLGM QLFCASSEES NGVKTIGIIK TPALRLKVNN LSLPHIGWNQ 130 140 150 160 170 180 ITFQKGHALF KNIPNNSRFY FVHSYMVPIN KYTLSTTNYG INFSSIIQKD NFFGVQFHPE 190 KSGNIGSQLL KNFLEI

References

[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed: 10993077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: APS.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000003 Genomic DNA. Translation: BAB12822.1.
RefSeq	NP_239936.1. NC_002528.1.
3D structure databases
ProteinModelPortal	P57204.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBUCT00000002807; EBBUCP00000002583; EBBUCG00000002807.
GeneID	1109506.
GenomeReviews	Gene locus BU103 in contig BA000003_GR.
KEGG	buc:BU103.
PATRIC	21243716. VBIBucAph127364_0111.
Phylogenomic databases
GeneTree	EBGT00050000008173.
HOGENOM	HBG292341.
OMA	YSEEDDI.
PhylomeDB	P57204.
ProtClustDB	PRK13170.
Enzyme and pathway databases
BioCyc	BSP107806:BU103-MONOMER.
Family and domain databases
HAMAP	MF_00278. HisH. [Tree]
InterPro	IPR017926. GATASE_1. IPR010139. Imidazole-glycPsynth_HisH. [Graphical view]
KO	K02501.
Pfam	PF00117. GATase. 1 hit. [Graphical view]
PIRSF	PIRSF000495. Amidotransf_hisH. 1 hit.
TIGRFAMs	TIGR01855. IMP_synth_hisH. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HIS5_BUCAI

Accession

Primary (citable) accession number: P57204

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	January 25, 2012
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program