P57203 (HIS7_BUCAI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Histidine biosynthesis bifunctional protein hisB

Including the following 2 domains:

1. Histidinol-phosphatase
   EC=3.1.3.15
2. Imidazoleglycerol-phosphate dehydratase
   Short name=IGPD
   EC=4.2.1.19

Gene names

Name:	hisB
Ordered Locus Names:	BU102

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)

Taxonomic identifier

107806 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	353 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate = 3-(imidazol-4-yl)-2-oxopropyl phosphate + H2O. HAMAP MF_01022 L-histidinol phosphate + H2O = L-histidinol + phosphate. HAMAP MF_01022
Pathway	Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 6/9. HAMAP MF_01022 Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 8/9.
Subcellular location	Cytoplasm By similarity HAMAP MF_01022.
Sequence similarities	In the N-terminal section; belongs to the histidinol-phosphatase family. In the C-terminal section; belongs to the imidazoleglycerol-phosphate dehydratase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Histidine biosynthesis
Cellular component	Cytoplasm
Molecular function	Hydrolase Lyase
Technical term	Complete proteome Multifunctional enzyme Reference proteome
Gene Ontology (GO)
Biological process	histidine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	histidinol-phosphatase activity Inferred from electronic annotation. Source: EC imidazoleglycerol-phosphate dehydratase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 353	353	Histidine biosynthesis bifunctional protein hisB HAMAP MF_01022		PRO_0000158201
Regions
	Region	1 – 164	164	Histidinol-phosphatase HAMAP MF_01022
	Region	165 – 353	189	Imidazoleglycerol-phosphate dehydratase HAMAP MF_01022

Sequences

	Sequence		Length	Mass (Da)
	P57203 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: 903BE7E53AD8DAA0	FASTA	353	40,836
	10 20 30 40 50 60 MKNKILFIDR DGTLIDEPIN TFQVDSINKL VFKKYVISSL RKLVELDYKL IMITNQDGLG 70 80 90 100 110 120 TESFPLQDFS TAHLFMLSVF RSEGVIFDDI LICPHFLDDD CVCRKPKIKM IEPWLDKIDL 130 140 150 160 170 180 KKSYVIGDRD TDMQLSNNLK IKGIKYKEDI CNWLHITKYI IKHNRYAEII RRTKETKVSI 190 200 210 220 230 240 KVWLDLEETS KIDTGVKFFD HMLEQLSVHS GICMNISVQG DLDIDDHHTI EDTGIVLGEA 250 260 270 280 290 300 LLQALGKKNG LSRFGFYLPM DESRSNCIMD ISNRPYLNFK AKFNHKMAGD LSTNMVEHFF 310 320 330 340 350 YSLCYSMKIT LHLYAEGKND HHCIESLFKV FGRTLRQAIK IEGNMLPTSK GIL

References

[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed: 10993077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: APS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000003 Genomic DNA. Translation: BAB12821.1.
RefSeq	NP_239935.1. NC_002528.1.
3D structure databases
ProteinModelPortal	P57203.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBUCT00000002825; EBBUCP00000002601; EBBUCG00000002825.
GeneID	1109505.
GenomeReviews	Gene locus BU102 in contig BA000003_GR.
KEGG	buc:BU102.
PATRIC	21243714. VBIBucAph127364_0110.
Phylogenomic databases
GeneTree	EBGT00050000008038.
HOGENOM	HBG289010.
OMA	EMVPHFF.
PhylomeDB	P57203.
ProtClustDB	PRK05446.
Enzyme and pathway databases
BioCyc	BSP107806:BU102-MONOMER.
Family and domain databases
HAMAP	MF_01022. Bifunc_HisB. [Tree]
InterPro	IPR023214. HAD-like_dom. IPR006549. HAD-SF_hydro_IIIA. IPR020566. His_synth_bifunc_HisB. IPR005954. HisB_N. IPR006543. Histidinol-phos. IPR000807. ImidazoleglycerolP_deHydtase. IPR020565. ImidazoleglycerP_deHydtase_CS. IPR013954. PNK3P. IPR020568. Ribosomal_S5_D2-typ_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.1000. HAD-like_dom. 1 hit.
KO	K01089.
PANTHER	PTHR23133:SF2. Imidazole-GPD. 1 hit.
Pfam	PF00475. IGPD. 1 hit. PF08645. PNK3P. 1 hit. [Graphical view]
SUPFAM	SSF56784. HAD-like_dom. 1 hit. SSF54211. Ribosomal_S5_D2-typ_fold. 2 hits.
TIGRFAMs	TIGR01662. HAD-SF-IIIA. 1 hit. TIGR01261. HisB_Nterm. 1 hit. TIGR01656. Histidinol-ppas. 1 hit.
PROSITE	PS00954. IGP_DEHYDRATASE_1. 1 hit. PS00955. IGP_DEHYDRATASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HIS7_BUCAI

Accession

Primary (citable) accession number: P57203

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	January 25, 2012
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program