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P57197 (DAPA_BUCAI) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 66. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Dihydrodipicolinate synthase
Short name=DHDPS
EC=4.2.1.52
Gene names
Name:dapA
Ordered Locus Names:BU096
OrganismBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Taxonomic identifier107806 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length294 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

L-aspartate 4-semialdehyde + pyruvate = dihydrodipicolinate + 2 H2O. HAMAP MF_00418

Pathway

Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 3/4. HAMAP MF_00418

Subunit structure

Homotetramer By similarity. HAMAP MF_00418

Subcellular location

Cytoplasm By similarity HAMAP MF_00418.

Sequence similarities

Belongs to the DHDPS family.

Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Diaminopimelate biosynthesis
Lysine biosynthesis
   Cellular componentCytoplasm
   LigandSchiff base
   Molecular functionLyase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological processdiaminopimelate biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functiondihydrodipicolinate synthase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 294294Dihydrodipicolinate synthase HAMAP MF_00418
PRO_0000103090

Regions

Region48 – 492Pyruvate binding By similarity

Sites

Active site1611Schiff-base intermediate with substrate By similarity
Binding site1061Pyruvate By similarity
Site1331Involved in proton transfer during cleavage By similarity

Sequences

Sequence LengthMass (Da)Tools
P57197 [UniParc].

Last modified December 1, 2000. Version 1.
Checksum: 4EFA4DDD19CF8733

FASTA29432,245
        10         20         30         40         50         60 
MFKGSIVALI TPMDEKGQIC RISLEKLINY HVASKTEAIV SIGTTGESAT LSQEEHINIV 

        70         80         90        100        110        120 
MLTLELADGR IPVIAGTGAN ATTEAISLTK RFEKSGVAGC LSVTPYYNRP TQEGLYQHFK 

       130        140        150        160        170        180 
AISENTELPQ ILYNVPSRTG CDLLPETVAK LSHFNNIIGI KEATGDLSRI HKIKELVKTN 

       190        200        210        220        230        240 
FLLISGDDAT ALDFMQLGGQ GVISVTANIA AKEMMEICSY ALKGDFINAR SINKRLMLLH 

       250        260        270        280        290 
EALFIEPNPI PVKWLAKKIG LIKSDTLRLP MTPVLDSTRF QLEKAIQYAN LKIS

« Hide

References

[1]"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
Nature 407:81-86(2000) [PubMed: 10993077] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: APS.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BA000003 Genomic DNA. Translation: BAB12815.1.
RefSeqNP_239929.1. NC_002528.1.

3D structure databases

ProteinModelPortalP57197.
SMRP57197. Positions 1-291.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBBUCT00000002785; EBBUCP00000002561; EBBUCG00000002785.
GeneID1109499.
GenomeReviewsGene locus BU096 in contig BA000003_GR.
KEGGbuc:BU096.
PATRIC21243702. VBIBucAph127364_0104.

Phylogenomic databases

GeneTreeEBGT00050000008107.
HOGENOMHBG358848.
OMAFMLCGGH.
PhylomeDBP57197.
ProtClustDBPRK03170.

Enzyme and pathway databases

BioCycBSP107806:BU096-MONOMER.

Family and domain databases

HAMAPMF_00418. DapA.
[Tree]
InterProIPR013785. Aldolase_TIM.
IPR002220. Dihydrodipicolinate_synth-like.
IPR020625. Dihydrodipicolinate_synth_AS.
IPR020624. Dihydrodipicolinate_synth_CS.
IPR005263. Dihydrodipicolinate_synth_DapA.
[Graphical view]
Gene3DG3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KOK01714.
PANTHERPTHR12128. DHDPS. 1 hit.
PfamPF00701. DHDPS. 1 hit.
[Graphical view]
PIRSFPIRSF001365. DHDPS. 1 hit.
PRINTSPR00146. DHPICSNTHASE.
TIGRFAMsTIGR00674. DapA. 1 hit.
PROSITEPS00665. DHDPS_1. 1 hit.
PS00666. DHDPS_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDAPA_BUCAI
AccessionPrimary (citable) accession number: P57197
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: January 25, 2012
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Buchnera aphidicola (subsp. Acyrthosiphon pisum)

Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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