P57195 (TKT_BUCAI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transketolase
Short name=TK
EC=2.2.1.1

Gene names

Name:	tkt
Ordered Locus Names:	BU094

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)

Taxonomic identifier

107806 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	665 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate = D-ribose 5-phosphate + D-xylulose 5-phosphate.
Cofactor	Binds 1 calcium ion per subunit By similarity. Binds 1 thiamine pyrophosphate per subunit By similarity.
Sequence similarities	Belongs to the transketolase family.

Ontologies

Keywords
Ligand	Calcium Metal-binding Thiamine pyrophosphate
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW transketolase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 665	665	Transketolase		PRO_0000191853
Sites
	Metal binding	155	1	Calcium By similarity
	Metal binding	185	1	Calcium By similarity
	Metal binding	187	1	Calcium; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	P57195 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: 1EAE37F97A34D2C7	FASTA	665	74,782
	10 20 30 40 50 60 MYSRKELANA IRMLSIDAVQ NAQSGHPGMP MGMADIAEVL WRSFLKHNPA NPNWNDRDRF 70 80 90 100 110 120 ILSNGHGSML LYSLLHLTGY NLPIEELKKF RQLNSKTPGH PETGETPGVE TTTGPLGQGL 130 140 150 160 170 180 ANAVGMAIAE RTLSSYFNRP GYDIINHYTW VFVGDGCLME GISHEVCSLA GTLNLGKLIV 190 200 210 220 230 240 FYDKNGISID GKTAHWFTDD TAKRFESYNW HVLDNIDGHD SESIERSIKQ AKLITNQPSI 250 260 270 280 290 300 IICNTIIGFG SPNKSGTAES HGAPLGEVEI SLIREQLKWN YPPFQIPKEI YKKWNFIEEG 310 320 330 340 350 360 SKLEKKWNEK FSLYQSKYPD LSTEYLRRIN KKLPVEWDRV TNNYISFLQK NRQSIASRKA 370 380 390 400 410 420 SQNTLEKYAM ILPELIGGSA DLSPSNLTMW SRCNSIKDNL SGNYIHYGVR EFGMTAIANG 430 440 450 460 470 480 ISHHGGFIPY TATFLMFVEY ARNAVRMAAL MCTKHIFVYT HDSIGLGEDG PTHQPVEQLS 490 500 510 520 530 540 SLRITPNIDV WRPSDQVETA VAWKKAIEKT SGPTALILSR QNLDQFERSS EQLENISYGA 550 560 570 580 590 600 YILYDSKKRL DIIFISTGSE LNVTLIAAKK LASLGYSVRV VSMPCTSVFD RQDASYKEFV 610 620 630 640 650 660 LPTYVAKRVA VEASIEDFWY KYVGINGVII GMKTFGESAP AEDLFKKFGF TVQNIFNKSL ILLKS

References

[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed: 10993077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: APS.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000003 Genomic DNA. Translation: BAB12813.1.
RefSeq	NP_239927.1. NC_002528.1.
3D structure databases
ProteinModelPortal	P57195.
SMR	P57195. Positions 3-663.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBUCT00000003216; EBBUCP00000002992; EBBUCG00000003216.
GeneID	1109497.
GenomeReviews	Gene locus BU094 in contig BA000003_GR.
KEGG	buc:BU094.
PATRIC	21243696. VBIBucAph127364_0101.
Phylogenomic databases
GeneTree	EBGT00050000007698.
HOGENOM	HBG565885.
OMA	GGYIIKD.
PhylomeDB	P57195.
ProtClustDB	PRK12753.
Enzyme and pathway databases
BioCyc	BSP107806:BU094-MONOMER.
Family and domain databases
InterPro	IPR009014. Transketo_C/Pyr-ferredox_oxred. IPR015941. Transketolase-like_C. IPR005475. Transketolase-like_Pyr-bd. IPR005478. Transketolase_bac-like. IPR020826. Transketolase_BS. IPR005476. Transketolase_C. IPR005474. Transketolase_N. [Graphical view]
Gene3D	G3DSA:3.40.50.920. Transketo_C_like. 1 hit.
KO	K00615.
Pfam	PF02779. Transket_pyr. 1 hit. PF02780. Transketolase_C. 1 hit. PF00456. Transketolase_N. 1 hit. [Graphical view]
SMART	SM00861. Transket_pyr. 1 hit. [Graphical view]
SUPFAM	SSF52922. Transketo_C_like. 1 hit.
TIGRFAMs	TIGR00232. Tktlase_bact. 1 hit.
PROSITE	PS00801. TRANSKETOLASE_1. 1 hit. PS00802. TRANSKETOLASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TKT_BUCAI

Accession

Primary (citable) accession number: P57195

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	January 25, 2012
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program