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P57190 (IPYR_BUCAI) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Inorganic pyrophosphatase

EC=3.6.1.1
Alternative name(s):
Pyrophosphate phospho-hydrolase
Short name=PPase
Gene names
Name:ppa
Ordered Locus Names:BU088
OrganismBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium) [Reference proteome] [HAMAP]
Taxonomic identifier107806 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera

Protein attributes

Sequence length182 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

Diphosphate + H2O = 2 phosphate. HAMAP-Rule MF_00209

Cofactor

Binds 4 magnesium ions per subunit. Other metal ions can support activity, but at a lower rate. Two magnesium ions are required for the activation of the enzyme and are present before substrate binds, two additional magnesium ions form complexes with substrate and product By similarity.

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00209.

Sequence similarities

Belongs to the PPase family.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandMagnesium
Metal-binding
   Molecular functionHydrolase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processphosphate-containing compound metabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functioninorganic diphosphatase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

magnesium ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 182182Inorganic pyrophosphatase HAMAP-Rule MF_00209
PRO_0000137484

Sites

Metal binding661Magnesium 1 By similarity
Metal binding711Magnesium 1 By similarity
Metal binding711Magnesium 2 By similarity
Metal binding1031Magnesium 1 By similarity

Sequences

Sequence LengthMass (Da)Tools
P57190 [UniParc].

Last modified December 1, 2000. Version 1.
Checksum: 2503EC5605F9C9B3

FASTA18220,763
        10         20         30         40         50         60 
MNLNKIVAGD DIPNDIYVII EISSNSSPIK YEVDKKSGML FVDRFIPTPM FYPCNYGYIN 

        70         80         90        100        110        120 
HTLSLDGDPL DVLVPSHYPI KSSCVIHCKP IGILNMQDES GDDAKIIAVP KSKICQEYKN 

       130        140        150        160        170        180 
INDISDISEL LKKQITHFFQ NYKTLEKEKW VEIIGWGNCQ DAKLEINDAY NRAKKNSIFL 


NK 

« Hide

References

[1]"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: APS.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000003 Genomic DNA. Translation: BAB12808.1.
RefSeqNP_239922.1. NC_002528.1.

3D structure databases

ProteinModelPortalP57190.
SMRP57190. Positions 2-174.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAB12808; BAB12808; BAB12808.
GeneID1109492.
KEGGbuc:BU088.
PATRIC21243686. VBIBucAph127364_0096.

Phylogenomic databases

eggNOGCOG0221.
HOGENOMHOG000236473.
KOK01507.
OMAGILHMTD.
OrthoDBEOG6NKR4X.
ProtClustDBPRK01250.

Enzyme and pathway databases

BioCycBAPH107806:GBZJ-88-MONOMER.

Family and domain databases

Gene3D3.90.80.10. 1 hit.
HAMAPMF_00209. Inorganic_PPase.
InterProIPR008162. Pyrophosphatase.
[Graphical view]
PANTHERPTHR10286. PTHR10286. 1 hit.
PfamPF00719. Pyrophosphatase. 1 hit.
[Graphical view]
SUPFAMSSF50324. SSF50324. 1 hit.
PROSITEPS00387. PPASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameIPYR_BUCAI
AccessionPrimary (citable) accession number: P57190
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: February 19, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Buchnera aphidicola (subsp. Acyrthosiphon pisum)

Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names