Reviewed, UniProtKB/Swiss-Prot P57173 (SYE_BUCAI)

Names and origin

Protein names

Recommended name:
    Glutamyl-tRNA synthetase
    EC=6.1.1.17
Alternative name(s):
    Glutamate--tRNA ligase
      Short name=GluRS

Gene names

Name:	gltX
Ordered Locus Names:	BU070

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (Acyrthosiphon pisum symbiotic bacterium) [Complete proteome] [HAMAP]

Taxonomic identifier

118099 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	467 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity.
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm HAMAP MF_00022.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP glutamate-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 467	467	Glutamyl-tRNA synthetase HAMAP MF_00022		PRO_0000119526
Regions
	Motif	9 – 19	11	"HIGH" region HAMAP MF_00022
	Motif	237 – 241	5	"KMSKS" region HAMAP MF_00022
Sites
	Binding site	240	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P57173-1 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: 1C35E3DA15A27593	FASTA	467	54,703
	10 20 30 40 50 60 MKVKTRFAPS PTGNLHIGSI RTALYSWLFA RHHNGKFVLR IEDTDLVRSE LISIDSIFNG 70 80 90 100 110 120 LKWLGLNWDE GPYFQTKRLD RYKEVINVML EKEDAYICVC SCQELEEIRK KQIEKGNKPR 130 140 150 160 170 180 YPGTCRNLNI KNISNQNHVI RFKNPLFGKV KFQDKIRGEI VFDNAELDDL VIQRSNGMPT 190 200 210 220 230 240 YNFCVVVDDM DMKITHVIRG EDHISNTPRQ INILNSLKVK IPVYAHLSMI LDEKGNKISK 250 260 270 280 290 300 RKNAINIIEY YENGFLPEAL LNYIIRLGWS YGDKEIFNLS EMKELFNLKS ITKSSSTVNF 310 320 330 340 350 360 KKLLWMNKYY INNSPLDYVS DCLKNYMEKK NINVEKGPDL ELLVKLFRSR HHTLKEITES 370 380 390 400 410 420 CRYFYEEINF FNHTVIEKYF TRKNCFILQT CYKKIEKLSI WDNKNISKII NDVSELIKVT 430 440 450 460 KKEISMILRI SMTGDICSPS ISTVILLIGK EKALLRINNV VNYIKNL

References

[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed: 10993077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tokyo 1998.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000003 Genomic DNA. Translation: BAB12791.1.
RefSeq	NP_239905.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1109475.
GenomeReviews	Gene locus BU070 in contig BA000003_GR.
KEGG	buc:BU070.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG628189.
OMA	DLVIQRS.
Enzyme and pathway databases
BioCyc	BSP107806:BU070-MON.
Family and domain databases
HAMAP	MF_00022. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR004527. Glu-tRNA-synth_Ic_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ic. IPR020061. Glu/Gln-tRNA-synth_Ic_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ic_cat-dom. IPR020060. Glu/Gln-tRNA-synth_Ic_N. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE_BUCAI

Accession

Primary (citable) accession number: P57173

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	December 15, 2009
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)