Reviewed, UniProtKB/Swiss-Prot P57171 (CYSK_BUCAI)

Names and origin

Protein names

Recommended name:
    Cysteine synthase
      Short name=CSase
    EC=2.5.1.47
Alternative name(s):
    O-acetylserine sulfhydrylase
    O-acetylserine (thiol)-lyase
      Short name=OAS-TL

Gene names

Name:	cysK
Ordered Locus Names:	BU066

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (Acyrthosiphon pisum symbiotic bacterium) [Complete proteome] [HAMAP]

Taxonomic identifier

118099 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	315 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	O(3)-acetyl-L-serine + H2S = L-cysteine + acetate.
Cofactor	Pyridoxal phosphate By similarity.
Pathway	Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-serine: step 2/2.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the cysteine synthase/cystathionine beta-synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Cysteine biosynthesis
Ligand	Pyridoxal phosphate
Molecular function	Transferase
Technical term	Allosteric enzyme Complete proteome
Gene Ontology (GO)
Biological process	cysteine biosynthetic process from serine Inferred from electronic annotation. Source: InterPro
Molecular function	cysteine synthase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro transferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 315	315	Cysteine synthase		PRO_0000167083
Regions
	Region	177 – 181	5	Pyridoxal phosphate binding By similarity
Sites
	Binding site	8	1	Allosteric inhibitor By similarity
	Binding site	72	1	Pyridoxal phosphate By similarity
	Binding site	269	1	Allosteric inhibitor; via amide nitrogen By similarity
	Binding site	273	1	Pyridoxal phosphate By similarity
Amino acid modifications
	Modified residue	42	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P57171-1 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: 84D371238D4238FF	FASTA	315	34,430
	10 20 30 40 50 60 MSKIYQDNSL TIGNTPLVRL NRIGNGNILV KIESRNPSFS VKCRIGANMI WHAEKNNNIN 70 80 90 100 110 120 KNVKLIEATS GNTGIALAYV AASRNYRLTL TMPETMSIER KKILKSLGAE LILTDGRYGM 130 140 150 160 170 180 KGAISKANDI ISRNPSKYFL LKQFENPANP EIHQITTGPE IWNDTNGNLD ILISAVGTGG 190 200 210 220 230 240 TITGITRYIK KIKRKKNLIS IAVEPSESPV ITQFLAGKAI EPGPHKIQGI GPGFIPKNLD 250 260 270 280 290 300 LTIIDQVITV SSEEAILTAK ELMKKEGILA GISSGAALYA AIKIQQQKKF SDKKIVVILP 310 SSGERYLSTE LFSEL

References

[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed: 10993077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tokyo 1998.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000003 Genomic DNA. Translation: BAB12789.1.
RefSeq	NP_239903.1.
3D structure databases
HSSP	HSSP built from PDB template 1OAS based on UniProtKB P12674.
SMR	P57171. Positions 2-315.
ModBase	Search...
Genome annotation databases
GeneID	1109473.
GenomeReviews	Gene locus BU066 in contig BA000003_GR.
KEGG	buc:BU066.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P57171.
OMA	KYFMPGQ.
Enzyme and pathway databases
BioCyc	BSP107806:BU066-MON.
Family and domain databases
InterPro	IPR001216. Cys_synth_BS. IPR005856. Cys_synthKM. IPR005859. CysK. IPR001926. PyrdxlP-dep_enz_bsu. [Graphical view]
Pfam	PF00291. PALP. 1 hit. [Graphical view]
TIGRFAMs	TIGR01139. cysK. 1 hit. TIGR01136. cysKM. 1 hit.
PROSITE	PS00901. CYS_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYSK_BUCAI

Accession

Primary (citable) accession number: P57171

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	November 3, 2009
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)