P57169 (CCA_BUCAI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
CCA-adding enzyme
EC=2.7.7.72

Alternative name(s):
CCA tRNA nucleotidyltransferase
tRNA CCA-pyrophosphorylase
tRNA adenylyl-/cytidylyl- transferase
tRNA nucleotidyltransferase
tRNA-NT

Gene names

Name:	cca
Ordered Locus Names:	BU061

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)

Taxonomic identifier

107806 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	414 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the addition and repair of the essential 3'-terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing inorganic pyrophosphate By similarity. HAMAP MF_01262
Catalytic activity	A tRNA precursor + 2 CTP + ATP = a tRNA with a 3' CCA end + 3 diphosphate. HAMAP MF_01262
Cofactor	Magnesium By similarity. HAMAP MF_01262
Miscellaneous	A single active site specifically recognizes both ATP and CTP and is responsible for their addition By similarity. HAMAP MF_01262
Sequence similarities	Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 2 subfamily.

Ontologies

Keywords
Biological process	RNA repair tRNA processing
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding RNA-binding
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	RNA repair Inferred from electronic annotation. Source: UniProtKB-KW tRNA 3'-terminal CCA addition Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW tRNA adenylyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 414	414	CCA-adding enzyme HAMAP MF_01262		PRO_0000139017
Sites
	Metal binding	21	1	Magnesium By similarity
	Metal binding	23	1	Magnesium By similarity
	Binding site	8	1	ATP or CTP; via amide nitrogen By similarity
	Binding site	11	1	ATP or CTP By similarity
	Binding site	91	1	ATP or CTP By similarity
	Binding site	137	1	ATP or CTP By similarity
	Binding site	140	1	ATP or CTP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P57169 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: 8CBF46515A47D4B7	FASTA	414	48,811
	10 20 30 40 50 60 MKIYLVGGAV RDSLLNLPVK DKDWVVVGGT EKILLERNFQ QVGKDFPVFL HPETHEEYAL 70 80 90 100 110 120 ARKERKSGKG YTGFDTDCNS DVTLEEDLIR RDLTINAIAQ DEYGNYIDPF QGKKDIECGL 130 140 150 160 170 180 IRHVSESFIE DPLRVLRVAR FAATLVHLGF KIAEETMLLM CIIVKKQELS YLTSNRIWNE 190 200 210 220 230 240 TEKALKTLNP HVYFQVLYEC NALHFFFPEM YFLYEKKNFL NRSFFKKFCN KNIILMGLAE 250 260 270 280 290 300 ISLLNKDIDV RFSYLCQFLS VNQIDRNYSK IFFDSYAASI IHSVCKRFKI PSYIRDIAVL 310 320 330 340 350 360 NTGFYFFLNT IHYQSSKNII NLFSKVDAWR KPDRVKKLAF LSNFNFLRNF KSEFFCIKSG 370 380 390 400 410 CFLEKCFSVV KNVSIKLILK KGFKGYEIKQ EITRLRIKKL EFWRIKNIKH RFYL

References

[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed: 10993077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: APS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000003 Genomic DNA. Translation: BAB12784.1.
RefSeq	NP_239898.1. NC_002528.1.
3D structure databases
ProteinModelPortal	P57169.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBUCT00000003222; EBBUCP00000002998; EBBUCG00000003222.
GeneID	1109468.
GenomeReviews	Gene locus BU061 in contig BA000003_GR.
KEGG	buc:BU061.
PATRIC	21243628. VBIBucAph127364_0070.
Phylogenomic databases
GeneTree	EBGT00050000008009.
HOGENOM	HBG635063.
OMA	GHGQKGL.
PhylomeDB	P57169.
ProtClustDB	PRK13298.
Enzyme and pathway databases
BioCyc	BSP107806:BU061-MONOMER.
Family and domain databases
HAMAP	MF_01262. CCA_bact_type2. [Tree]
InterPro	IPR012006. CCA_bact. IPR002646. PolA_pol_head_dom. [Graphical view]
KO	K00974.
Pfam	PF01743. PolyA_pol. 1 hit. [Graphical view]
PIRSF	PIRSF000813. CCA_bact. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CCA_BUCAI

Accession

Primary (citable) accession number: P57169

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	January 25, 2012
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program