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P57166

- TSAD_BUCAI

UniProt

P57166 - TSAD_BUCAI

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Protein

tRNA N6-adenosine threonylcarbamoyltransferase

Gene

tsaD

Organism
Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. Is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaE and TsaB. TsaD likely plays a direct catalytic role in this reaction.UniRule annotation

Catalytic activityi

L-threonylcarbamoyladenylate + adenine(37) in tRNA = AMP + N(6)-L-threonylcarbamoyladenine(37) in tRNA.UniRule annotation

Cofactori

Binds 1 Fe2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi110 – 1101IronUniRule annotation
Metal bindingi114 – 1141IronUniRule annotation
Binding sitei166 – 1661SubstrateUniRule annotation
Binding sitei179 – 1791Substrate; via amide nitrogenUniRule annotation
Binding sitei271 – 2711SubstrateUniRule annotation
Metal bindingi300 – 3001IronUniRule annotation

GO - Molecular functioni

  1. iron ion binding Source: UniProtKB-HAMAP
  2. metalloendopeptidase activity Source: InterPro
  3. transferase activity, transferring acyl groups other than amino-acyl groups Source: UniProtKB-HAMAP

GO - Biological processi

  1. threonylcarbamoyladenosine biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BioCyciBAPH107806:GBZJ-58-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA N6-adenosine threonylcarbamoyltransferaseUniRule annotation (EC:2.6.99.4UniRule annotation)
Alternative name(s):
N6-L-threonylcarbamoyladenine synthaseUniRule annotation
Short name:
t(6)A synthaseUniRule annotation
t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaDUniRule annotation
tRNA threonylcarbamoyladenosine biosynthesis protein TsaDUniRule annotation
Gene namesi
Name:tsaDUniRule annotation
Synonyms:gcp
Ordered Locus Names:BU058
OrganismiBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Taxonomic identifieri107806 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
ProteomesiUP000001806: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 336336tRNA N6-adenosine threonylcarbamoyltransferasePRO_0000096959Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP57166.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni133 – 1375Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the KAE1 / TsaD family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0533.
HOGENOMiHOG000109568.
KOiK01409.
OMAiINDSPRG.
OrthoDBiEOG6K402S.

Family and domain databases

HAMAPiMF_01445. TsaD.
InterProiIPR000905. Gcp-like_dom.
IPR017861. KAE1/YgjD.
IPR017860. Peptidase_M22_CS.
IPR022450. TsaD.
[Graphical view]
PfamiPF00814. Peptidase_M22. 1 hit.
[Graphical view]
PRINTSiPR00789. OSIALOPTASE.
TIGRFAMsiTIGR00329. gcp_kae1. 1 hit.
TIGR03723. T6A_YgjD. 1 hit.
PROSITEiPS01016. GLYCOPROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P57166-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRILGIETSC DDTGIAIYDT NKGLLINEIY NQRKLNNIYG GIIPELASRE
60 70 80 90 100
HMEAMIVLLN KIFKKKNIYK YVDMIAYTAG PGLIGSLLVG ATFACSLGLS
110 120 130 140 150
LNIPVLPVHH MEAHLLSPML DYKTIQFPFI GLLVSGKHTQ IIGAHKFGEY
160 170 180 190 200
EILGNCLDDA AGEAFDKTAK LLGLKYPGGL ELSKLASKGI KDYFYFPRPM
210 220 230 240 250
IHHSDLNFSF SGLKTFAAQT IKKSSKSMQE KANIAKAFED AVIDILLIKT
260 270 280 290 300
KKALKKQKWK RLVIAGGVSA NQKLRKKSEI MVKKNFNGTV FYSSLEFCTD
310 320 330
NAAMIAYLGS LRQKEARNSQ LEILVKPKWS IDDLCF
Length:336
Mass (Da):37,438
Last modified:December 1, 2000 - v1
Checksum:i11FE9A328D885971
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000003 Genomic DNA. Translation: BAB12781.1.
RefSeqiNP_239895.1. NC_002528.1.

Genome annotation databases

EnsemblBacteriaiBAB12781; BAB12781; BAB12781.
GeneIDi1109465.
KEGGibuc:BU058.
PATRICi21243622. VBIBucAph127364_0067.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000003 Genomic DNA. Translation: BAB12781.1 .
RefSeqi NP_239895.1. NC_002528.1.

3D structure databases

ProteinModelPortali P57166.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAB12781 ; BAB12781 ; BAB12781 .
GeneIDi 1109465.
KEGGi buc:BU058.
PATRICi 21243622. VBIBucAph127364_0067.

Phylogenomic databases

eggNOGi COG0533.
HOGENOMi HOG000109568.
KOi K01409.
OMAi INDSPRG.
OrthoDBi EOG6K402S.

Enzyme and pathway databases

BioCyci BAPH107806:GBZJ-58-MONOMER.

Family and domain databases

HAMAPi MF_01445. TsaD.
InterProi IPR000905. Gcp-like_dom.
IPR017861. KAE1/YgjD.
IPR017860. Peptidase_M22_CS.
IPR022450. TsaD.
[Graphical view ]
Pfami PF00814. Peptidase_M22. 1 hit.
[Graphical view ]
PRINTSi PR00789. OSIALOPTASE.
TIGRFAMsi TIGR00329. gcp_kae1. 1 hit.
TIGR03723. T6A_YgjD. 1 hit.
PROSITEi PS01016. GLYCOPROTEASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
    Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
    Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: APS.

Entry informationi

Entry nameiTSAD_BUCAI
AccessioniPrimary (citable) accession number: P57166
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: October 29, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Buchnera aphidicola (subsp. Acyrthosiphon pisum)
    Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3