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P57166

- TSAD_BUCAI

UniProt

P57166 - TSAD_BUCAI

Protein

tRNA N6-adenosine threonylcarbamoyltransferase

Gene

tsaD

Organism
Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 84 (01 Oct 2014)
      Sequence version 1 (01 Dec 2000)
      Previous versions | rss
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    Functioni

    Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. Is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaE and TsaB. TsaD likely plays a direct catalytic role in this reaction.UniRule annotation

    Catalytic activityi

    L-threonylcarbamoyladenylate + adenine(37) in tRNA = AMP + N(6)-L-threonylcarbamoyladenine(37) in tRNA.UniRule annotation

    Cofactori

    Binds 1 Fe2+ ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi110 – 1101IronUniRule annotation
    Metal bindingi114 – 1141IronUniRule annotation
    Binding sitei166 – 1661SubstrateUniRule annotation
    Binding sitei179 – 1791Substrate; via amide nitrogenUniRule annotation
    Binding sitei271 – 2711SubstrateUniRule annotation
    Metal bindingi300 – 3001IronUniRule annotation

    GO - Molecular functioni

    1. iron ion binding Source: UniProtKB-HAMAP
    2. metalloendopeptidase activity Source: InterPro
    3. transferase activity, transferring acyl groups other than amino-acyl groups Source: UniProtKB-HAMAP

    GO - Biological processi

    1. threonylcarbamoyladenosine biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Acyltransferase, Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    Iron, Metal-binding

    Enzyme and pathway databases

    BioCyciBAPH107806:GBZJ-58-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA N6-adenosine threonylcarbamoyltransferaseUniRule annotation (EC:2.6.99.4UniRule annotation)
    Alternative name(s):
    N6-L-threonylcarbamoyladenine synthaseUniRule annotation
    Short name:
    t(6)A synthaseUniRule annotation
    t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaDUniRule annotation
    tRNA threonylcarbamoyladenosine biosynthesis protein TsaDUniRule annotation
    Gene namesi
    Name:tsaDUniRule annotation
    Synonyms:gcp
    Ordered Locus Names:BU058
    OrganismiBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
    Taxonomic identifieri107806 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
    ProteomesiUP000001806: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 336336tRNA N6-adenosine threonylcarbamoyltransferasePRO_0000096959Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliP57166.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni133 – 1375Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the KAE1 / TsaD family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0533.
    HOGENOMiHOG000109568.
    KOiK01409.
    OMAiINDSPRG.
    OrthoDBiEOG6K402S.

    Family and domain databases

    HAMAPiMF_01445. TsaD.
    InterProiIPR000905. Gcp-like_dom.
    IPR017861. KAE1/YgjD.
    IPR017860. Peptidase_M22_CS.
    IPR022450. TsaD.
    [Graphical view]
    PfamiPF00814. Peptidase_M22. 1 hit.
    [Graphical view]
    PRINTSiPR00789. OSIALOPTASE.
    TIGRFAMsiTIGR00329. gcp_kae1. 1 hit.
    TIGR03723. T6A_YgjD. 1 hit.
    PROSITEiPS01016. GLYCOPROTEASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P57166-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRILGIETSC DDTGIAIYDT NKGLLINEIY NQRKLNNIYG GIIPELASRE    50
    HMEAMIVLLN KIFKKKNIYK YVDMIAYTAG PGLIGSLLVG ATFACSLGLS 100
    LNIPVLPVHH MEAHLLSPML DYKTIQFPFI GLLVSGKHTQ IIGAHKFGEY 150
    EILGNCLDDA AGEAFDKTAK LLGLKYPGGL ELSKLASKGI KDYFYFPRPM 200
    IHHSDLNFSF SGLKTFAAQT IKKSSKSMQE KANIAKAFED AVIDILLIKT 250
    KKALKKQKWK RLVIAGGVSA NQKLRKKSEI MVKKNFNGTV FYSSLEFCTD 300
    NAAMIAYLGS LRQKEARNSQ LEILVKPKWS IDDLCF 336
    Length:336
    Mass (Da):37,438
    Last modified:December 1, 2000 - v1
    Checksum:i11FE9A328D885971
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000003 Genomic DNA. Translation: BAB12781.1.
    RefSeqiNP_239895.1. NC_002528.1.

    Genome annotation databases

    EnsemblBacteriaiBAB12781; BAB12781; BAB12781.
    GeneIDi1109465.
    KEGGibuc:BU058.
    PATRICi21243622. VBIBucAph127364_0067.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000003 Genomic DNA. Translation: BAB12781.1 .
    RefSeqi NP_239895.1. NC_002528.1.

    3D structure databases

    ProteinModelPortali P57166.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAB12781 ; BAB12781 ; BAB12781 .
    GeneIDi 1109465.
    KEGGi buc:BU058.
    PATRICi 21243622. VBIBucAph127364_0067.

    Phylogenomic databases

    eggNOGi COG0533.
    HOGENOMi HOG000109568.
    KOi K01409.
    OMAi INDSPRG.
    OrthoDBi EOG6K402S.

    Enzyme and pathway databases

    BioCyci BAPH107806:GBZJ-58-MONOMER.

    Family and domain databases

    HAMAPi MF_01445. TsaD.
    InterProi IPR000905. Gcp-like_dom.
    IPR017861. KAE1/YgjD.
    IPR017860. Peptidase_M22_CS.
    IPR022450. TsaD.
    [Graphical view ]
    Pfami PF00814. Peptidase_M22. 1 hit.
    [Graphical view ]
    PRINTSi PR00789. OSIALOPTASE.
    TIGRFAMsi TIGR00329. gcp_kae1. 1 hit.
    TIGR03723. T6A_YgjD. 1 hit.
    PROSITEi PS01016. GLYCOPROTEASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
      Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
      Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: APS.

    Entry informationi

    Entry nameiTSAD_BUCAI
    AccessioniPrimary (citable) accession number: P57166
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: December 1, 2000
    Last modified: October 1, 2014
    This is version 84 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Buchnera aphidicola (subsp. Acyrthosiphon pisum)
      Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3