Reviewed, UniProtKB/Swiss-Prot P57164 (PRIM_BUCAI)

Names and origin

Protein names

Recommended name:
    DNA primase
    EC=2.7.7.-

Gene names

Name:	dnaG
Ordered Locus Names:	BU056

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (Acyrthosiphon pisum symbiotic bacterium) [Complete proteome] [HAMAP]

Taxonomic identifier

118099 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	577 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis.
Cofactor	Binds 1 zinc ion per monomer By similarity.
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the DNA primase family.

Ontologies

Keywords
Biological process	DNA replication Transcription
Cellular component	DNA-directed RNA polymerase Primosome
Domain	Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA replication, synthesis of RNA primer Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	alpha DNA polymerase:primase complex Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA primase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 577	577	DNA primase		PRO_0000180481
Regions
	Zinc finger	40 – 64	25	CHC2-type By similarity

Sequences

	Sequence		Length	Mass (Da)
	P57164-1 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: 67A774B58CB12E87	FASTA	577	67,772
	10 20 30 40 50 60 MSGKIPKYFI TELLSRTNII ELINTRLELK KYGKNYQTNC PFHHDKTPSF TVSNEKQFYY 70 80 90 100 110 120 CFGCNAHGNA IDFLIQYEHL SFIESIEELA LIHGVKIPFE NTVQNSIYVK KQKLYLLMEK 130 140 150 160 170 180 ICKLYKKNIN VTHLANKYLA RRGINQNMID FFLIGFSSLK WNEFYKKINI SKEFEQELLI 190 200 210 220 230 240 NNIIATDKNG YIYDRFQGRI IFPIQDNHGR IIGFGGRSLN DMSPKYLNSP ETDIFYKRKQ 250 260 270 280 290 300 IYGLYQVIKK CSKPVYLLVV EGYIDVITLT QYNIDYAVSI LGTSTTTEHI QLLFKNTDII 310 320 330 340 350 360 ICCYDGDDAG KNAAWKTLKK ALPYISDKKT LKFILLPNQE DPDTIIRKEG REKFQKRIDN 370 380 390 400 410 420 AITMSKFFFK NILKNINLSS DDDKFHLSVH ALPLINTISS DTIRIYLRQI LARMIGILDD 430 440 450 460 470 480 NQFEKFLYEK ETKNTQKTYF QIKRTPMRTL IGLLVQNPSL AKLVPVTMKF KNLQIKGLSI 490 500 510 520 530 540 FLEILQTCRD NPNIHTGQLL ELYRNTTIIN VLKILARWDH MIIQKETQNM FLDLLMNIHD 550 560 570 KILEKRREYL IAKERKKGLQ MNEKKEIWSI NKKLSKR

References

[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed: 10993077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tokyo 1998.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000003 Genomic DNA. Translation: BAB12779.1.
RefSeq	NP_239893.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1109463.
GenomeReviews	Gene locus BU056 in contig BA000003_GR.
KEGG	buc:BU056.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P57164.
OMA	DDIADKD
Enzyme and pathway databases
BioCyc	BSP107806:BU056-MON.
Family and domain databases
InterPro	IPR016136. DNA_helicase_N/primase_C. IPR013264. DNA_primase_core_N. IPR019475. DNA_primase_DnaB_bd. IPR006295. DNA_primase_DnaG. IPR013173. DNA_primase_DnaG_DnaB-bd. IPR006154. Toprim_dom_subgr. IPR006171. Toprim_domain. IPR002694. Znf_CHC2. [Graphical view]
Gene3D	G3DSA:1.10.860.10. DnaB_N. 1 hit. G3DSA:3.90.980.10. Toprim_N. 1 hit. G3DSA:3.90.580.10. Znf_CHC2. 1 hit.
Pfam	PF10410. DnaB_bind. 1 hit. PF08278. DnaG_DnaB_bind. 1 hit. PF01751. Toprim. 1 hit. PF08275. Toprim_N. 1 hit. PF01807. zf-CHC2. 1 hit. [Graphical view]
SMART	SM00766. DnaG_DnaB_bind. 1 hit. SM00493. TOPRIM. 1 hit. SM00400. ZnF_CHCC. 1 hit. [Graphical view]
TIGRFAMs	TIGR01391. dnaG. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PRIM_BUCAI

Accession

Primary (citable) accession number: P57164

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	November 24, 2009
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)