P57163 (RPOD_BUCAI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
RNA polymerase sigma factor RpoD

Alternative name(s):
Sigma-70

Gene names

Name:	rpoD
Ordered Locus Names:	BU055

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium) [Reference proteome] [HAMAP]

Taxonomic identifier

107806 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera › Buchnera aphidicola › Buchnera aphidicola (Acyrthosiphon pisum)

Protein attributes

Sequence length	612 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium.
Sequence similarities	Belongs to the sigma-70 factor family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Ligand	DNA-binding
Molecular function	Sigma factor
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA-dependent transcription, initiation Inferred from electronic annotation. Source: InterPro
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro sigma factor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 612	612	RNA polymerase sigma factor RpoD		PRO_0000093877
Regions
	DNA binding	572 – 591	20	H-T-H motif By similarity
	Motif	402 – 415	14	Polymerase core binding Potential

Sequences

	Sequence		Length	Mass (Da)
	P57163 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: 6BDD03CC2E1D112A	FASTA	612	71,050
	10 20 30 40 50 60 MDQNPQSQLK LLVTHGKEQG YLTYSEVNDH LPEDIIDSEQ IDDIIQMIND MGIPVVEEAP 70 80 90 100 110 120 DADDLILNEI NTDTDEDAVE AATQVLSSVE SELGRTTDPV RMYMREMGTV ELLTREGEID 130 140 150 160 170 180 IAKRIEEGIN QVQCSVSEYP EAITYLLEQY DRVKTGQIRL SDIITGFVDP NAEEIFPPTA 190 200 210 220 230 240 IHIGSELLDE QQNNEEDEEN NQEDHEDDHS IDPELANEKF SELRIQYNNT NNTIKNKNRT 250 260 270 280 290 300 HKDSLLEIYN LSEVFKQFRL VPKQFDHLVN NMRHMMERVR KQERIIIKLC VEICKMPKKN 310 320 330 340 350 360 FIKIFPIKKI NYLWFIREQN TNQPWSENLK KVKEDVFISV KKLIKIEEET GLTIEQVKDI 370 380 390 400 410 420 NKRMSIGEAK ARRAKKEMVE ANLRLVISIA KKYTNRGLQF LDLIQEGNIG LMKAVDKFEY 430 440 450 460 470 480 RRGYKFSTYA TWWIRQAITR SIADQARTIR IPVHMIETIN KLNRISRQML QEIGREPTPE 490 500 510 520 530 540 ELSEKMLIPE DKIRKVLKIA KEPISMETPI GDDDDSHLGD FIEDTTLELP LDSATSESLR 550 560 570 580 590 600 SATHDVLSGL TAREAKVLRM RFGIDMNTDH TLEEVGKQFD VTRERIRQIE AKALRKLRHP 610 SRSEVLRSFL DD

References

[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: APS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000003 Genomic DNA. Translation: BAB12778.1.
RefSeq	NP_239892.1. NC_002528.1.
3D structure databases
ProteinModelPortal	P57163.
SMR	P57163. Positions 116-445, 545-612.
ModBase	Search...
Proteomic databases
PRIDE	P57163.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAB12778; BAB12778; BAB12778.
GeneID	1109462.
KEGG	buc:BU055.
PATRIC	21243616. VBIBucAph127364_0064.
Phylogenomic databases
eggNOG	COG0568.
HOGENOM	HOG000270272.
KO	K03086.
OMA	IEMNTDH.
ProtClustDB	PRK05658.
Enzyme and pathway databases
BioCyc	BAPH107806:GBZJ-55-MONOMER.
Family and domain databases
Gene3D	1.10.10.10. 2 hits.
InterPro	IPR014284. RNA_pol_sigma-70_dom. IPR000943. RNA_pol_sigma70. IPR009042. RNA_pol_sigma70_r1_2. IPR007627. RNA_pol_sigma70_r2. IPR007624. RNA_pol_sigma70_r3. IPR007630. RNA_pol_sigma70_r4. IPR007631. RNA_pol_sigma_70_non-ess. IPR007127. RNA_pol_sigma_70_r1_1. IPR013325. RNA_pol_sigma_r2. IPR013324. RNA_pol_sigma_r3_r4. IPR012760. RNA_pol_sigma_RpoD_C. IPR011991. WHTH_DNA-bd_dom. [Graphical view]
Pfam	PF04546. Sigma70_ner. 1 hit. PF03979. Sigma70_r1_1. 1 hit. PF00140. Sigma70_r1_2. 1 hit. PF04542. Sigma70_r2. 1 hit. PF04539. Sigma70_r3. 1 hit. PF04545. Sigma70_r4. 1 hit. [Graphical view]
PRINTS	PR00046. SIGMA70FCT.
SUPFAM	SSF88946. Sigma_r2. 1 hit. SSF88659. Sigma_r3_r4. 2 hits.
TIGRFAMs	TIGR02393. RpoD_Cterm. 1 hit. TIGR02937. sigma70-ECF. 1 hit.
PROSITE	PS00715. SIGMA70_1. 1 hit. PS00716. SIGMA70_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RPOD_BUCAI

Accession

Primary (citable) accession number: P57163

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	May 29, 2013
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program