Reviewed, UniProtKB/Swiss-Prot P57163 (RPOD_BUCAI)

Names and origin

Protein names

Recommended name:
    RNA polymerase sigma factor rpoD
Alternative name(s):
    Sigma-70

Gene names

Name:	rpoD
Ordered Locus Names:	BU055

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (Acyrthosiphon pisum symbiotic bacterium) [Complete proteome] [HAMAP]

Taxonomic identifier

118099 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	612 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium.
Sequence similarities	Belongs to the sigma-70 factor family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Ligand	DNA-binding
Molecular function	Sigma factor
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription initiation Inferred from electronic annotation. Source: InterPro
Molecular function	sigma factor activity Inferred from electronic annotation. Source: UniProtKB-KW transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 612	612	RNA polymerase sigma factor rpoD		PRO_0000093877
Regions
	DNA binding	572 – 591	20	H-T-H motif By similarity
	Motif	402 – 415	14	Polymerase core binding Potential

Sequences

	Sequence		Length	Mass (Da)
	P57163-1 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: 6BDD03CC2E1D112A	FASTA	612	71,050
	10 20 30 40 50 60 MDQNPQSQLK LLVTHGKEQG YLTYSEVNDH LPEDIIDSEQ IDDIIQMIND MGIPVVEEAP 70 80 90 100 110 120 DADDLILNEI NTDTDEDAVE AATQVLSSVE SELGRTTDPV RMYMREMGTV ELLTREGEID 130 140 150 160 170 180 IAKRIEEGIN QVQCSVSEYP EAITYLLEQY DRVKTGQIRL SDIITGFVDP NAEEIFPPTA 190 200 210 220 230 240 IHIGSELLDE QQNNEEDEEN NQEDHEDDHS IDPELANEKF SELRIQYNNT NNTIKNKNRT 250 260 270 280 290 300 HKDSLLEIYN LSEVFKQFRL VPKQFDHLVN NMRHMMERVR KQERIIIKLC VEICKMPKKN 310 320 330 340 350 360 FIKIFPIKKI NYLWFIREQN TNQPWSENLK KVKEDVFISV KKLIKIEEET GLTIEQVKDI 370 380 390 400 410 420 NKRMSIGEAK ARRAKKEMVE ANLRLVISIA KKYTNRGLQF LDLIQEGNIG LMKAVDKFEY 430 440 450 460 470 480 RRGYKFSTYA TWWIRQAITR SIADQARTIR IPVHMIETIN KLNRISRQML QEIGREPTPE 490 500 510 520 530 540 ELSEKMLIPE DKIRKVLKIA KEPISMETPI GDDDDSHLGD FIEDTTLELP LDSATSESLR 550 560 570 580 590 600 SATHDVLSGL TAREAKVLRM RFGIDMNTDH TLEEVGKQFD VTRERIRQIE AKALRKLRHP 610 SRSEVLRSFL DD

References

[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed: 10993077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tokyo 1998.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000003 Genomic DNA. Translation: BAB12778.1.
RefSeq	NP_239892.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1109462.
GenomeReviews	Gene locus BU055 in contig BA000003_GR.
KEGG	buc:BU055.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P57163.
OMA	HPSRSET
Enzyme and pathway databases
BioCyc	BSP107806:BU055-MON.
Family and domain databases
InterPro	IPR014284. RNA_pol_sigma-70. IPR000943. RNA_pol_sigma70. IPR009042. RNA_pol_sigma70_r1_2. IPR007627. RNA_pol_sigma70_r2. IPR007624. RNA_pol_sigma70_r3. IPR007630. RNA_pol_sigma70_r4. IPR007631. RNA_pol_sigma_70_non-ess. IPR007127. RNA_pol_sigma_70_r1.1. IPR013325. RNA_pol_sigma_r2. IPR013324. RNA_pol_sigma_r3_r4. IPR012760. RNA_pol_sigma_RpoD_C. IPR011991. Wing_hlx_DNA_bd. [Graphical view]
Gene3D	G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 2 hits.
Pfam	PF04546. Sigma70_ner. 1 hit. PF03979. Sigma70_r1_1. 1 hit. PF00140. Sigma70_r1_2. 1 hit. PF04542. Sigma70_r2. 1 hit. PF04539. Sigma70_r3. 1 hit. PF04545. Sigma70_r4. 1 hit. [Graphical view]
PRINTS	PR00046. SIGMA70FCT.
TIGRFAMs	TIGR02393. RpoD_Cterm. 1 hit. TIGR02937. sigma70-ECF. 1 hit.
PROSITE	PS00715. SIGMA70_1. 1 hit. PS00716. SIGMA70_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RPOD_BUCAI

Accession

Primary (citable) accession number: P57163

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	November 24, 2009
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)