P57157 (ARGB_BUCAI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acetylglutamate kinase
EC=2.7.2.8

Alternative name(s):
N-acetyl-L-glutamate 5-phosphotransferase
NAG kinase
Short name=AGK

Gene names

Name:	argB
Ordered Locus Names:	BU049

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)

Taxonomic identifier

107806 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	257 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + N-acetyl-L-glutamate = ADP + N-acetyl-L-glutamate 5-phosphate. HAMAP MF_00082_B
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 2/4. HAMAP MF_00082_B
Subunit structure	Homodimer By similarity. HAMAP MF_00082_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00082_B.
Sequence similarities	Belongs to the acetylglutamate kinase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW acetylglutamate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 257	257	Acetylglutamate kinase HAMAP MF_00082_B		PRO_0000112598
Regions
	Nucleotide binding	180 – 185	6	ATP By similarity
	Nucleotide binding	208 – 210	3	ATP By similarity
	Region	43 – 44	2	Substrate binding By similarity
Sites
	Binding site	65	1	Substrate By similarity
	Binding site	157	1	Substrate By similarity
	Site	7	1	Transition state stabilizer By similarity
	Site	216	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	P57157 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: 54EF26B46D6C064B	FASTA	257	27,811
	10 20 30 40 50 60 MNPLVIKLGG VLLESDDAMK RLFEALVDYQ KFYKRHVSVI HGGGRLIDNL MNKLSLPVKK 70 80 90 100 110 120 KNGLRITPSE HINIITGALA GTANKTLLAW ALKYNINAIG LCLADGGSVD VERLDENLGH 130 140 150 160 170 180 VGKAIPGSPL FLKKLFKEGT IPIISSIGIT KDGLLMNVNA DLAATALATT LQANLILLSD 190 200 210 220 230 240 ISSILDGKGQ RITEIDSIQA EKLIMQGIIT NGMIVKVRAA LEAARVLRRP VDIASWQNTE 250 KLKLLFNGVN IGTRVYV

References

[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed: 10993077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: APS.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000003 Genomic DNA. Translation: BAB12772.1.
RefSeq	NP_239886.1. NC_002528.1.
3D structure databases
ProteinModelPortal	P57157.
SMR	P57157. Positions 1-255.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBUCT00000002936; EBBUCP00000002712; EBBUCG00000002936.
GeneID	1109456.
GenomeReviews	Gene locus BU049 in contig BA000003_GR.
KEGG	buc:BU049.
PATRIC	21243604. VBIBucAph127364_0058.
Phylogenomic databases
GeneTree	EBGT00050000008092.
HOGENOM	HBG497643.
OMA	GGCLVDD.
PhylomeDB	P57157.
ProtClustDB	PRK00942.
Enzyme and pathway databases
BioCyc	BSP107806:BU049-MONOMER.
Family and domain databases
HAMAP	MF_00082_B. ArgB_B. [Tree]
InterPro	IPR004662. AcgluKinase. IPR001048. Asp/Glu/Uridylate_kinase. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit.
KO	K00930.
Pfam	PF00696. AA_kinase. 1 hit. [Graphical view]
PIRSF	PIRSF000728. NAGK. 1 hit.
SUPFAM	SSF53633. Aa_kinase. 1 hit.
TIGRFAMs	TIGR00761. ArgB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ARGB_BUCAI

Accession

Primary (citable) accession number: P57157

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	January 25, 2012
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program