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P57154

- METF_BUCAI

UniProt

P57154 - METF_BUCAI

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Protein

5,10-methylenetetrahydrofolate reductase

Gene
metF, BU046
Organism
Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic activityi

5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H.

Cofactori

FAD By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei28 – 281Proton donor/acceptor By similarity
Binding sitei88 – 881FAD By similarity
Binding sitei120 – 1201Substrate By similarity
Binding sitei152 – 1521FAD By similarity
Binding sitei183 – 1831Substrate By similarity
Binding sitei223 – 2231Substrate By similarity
Binding sitei275 – 2751Substrate By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi28 – 336NAD By similarity
Nucleotide bindingi59 – 624FAD By similarity
Nucleotide bindingi59 – 602NAD By similarity
Nucleotide bindingi118 – 1203FAD By similarity
Nucleotide bindingi131 – 1322FAD By similarity
Nucleotide bindingi156 – 1594FAD By similarity
Nucleotide bindingi165 – 1728FAD By similarity

GO - Molecular functioni

  1. methylenetetrahydrofolate reductase (NAD(P)H) activity Source: UniProtKB-EC

GO - Biological processi

  1. methionine biosynthetic process Source: UniProtKB-KW
  2. tetrahydrofolate interconversion Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Methionine biosynthesis

Keywords - Ligandi

FAD, Flavoprotein, NAD, NADP

Enzyme and pathway databases

BioCyciBAPH107806:GBZJ-46-MONOMER.
UniPathwayiUPA00193.

Names & Taxonomyi

Protein namesi
Recommended name:
5,10-methylenetetrahydrofolate reductase (EC:1.5.1.20)
Gene namesi
Name:metF
Ordered Locus Names:BU046
OrganismiBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Taxonomic identifieri107806 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
ProteomesiUP000001806: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 2922925,10-methylenetetrahydrofolate reductasePRO_0000190258Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP57154.
SMRiP57154. Positions 8-292.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0685.
HOGENOMiHOG000246232.
KOiK00297.
OMAiFEFFPTK.
OrthoDBiEOG6D2KVW.

Family and domain databases

Gene3Di3.20.20.220. 1 hit.
InterProiIPR029041. FAD-linked_oxidoreductase-like.
IPR003171. Mehydrof_redctse.
IPR004620. MTHF_reductase_bac.
[Graphical view]
PfamiPF02219. MTHFR. 1 hit.
[Graphical view]
SUPFAMiSSF51730. SSF51730. 1 hit.
TIGRFAMsiTIGR00676. fadh2. 1 hit.

Sequencei

Sequence statusi: Complete.

P57154-1 [UniParc]FASTAAdd to Basket

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MKNYSQYHQD IMNQKLENIR NNIQCSFEFF PPKNSFLEEN LWSTVNRLSL    50
LKPKFFSVTY GANTGEREKT YDTVQKIYKK TGIITAAHLT CIDSTPHKLK 100
EIAYNYWNSG IKSIVALRGD TLEKDYRHTM YAVDLVLLLK KIADFDISVA 150
AYPELHPESK NVKSDILNLK KKVDAGASRA ITQFFFNIES YLRFRDNCIK 200
NKINIDIIPG ILPVCNFQKL KRFSSMTNVK IPKWMLDMFN GLDDDIFTQK 250
IIGSSIAIDM VKKLSCEGVK NFHFYTLNQS DITYSICHIL GL 292
Length:292
Mass (Da):33,730
Last modified:December 1, 2000 - v1
Checksum:i1D2A9E1B4D6E0DA9
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000003 Genomic DNA. Translation: BAB12769.1.
RefSeqiNP_239883.1. NC_002528.1.

Genome annotation databases

EnsemblBacteriaiBAB12769; BAB12769; BAB12769.
GeneIDi1109550.
KEGGibuc:BU046.
PATRICi21243598. VBIBucAph127364_0055.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000003 Genomic DNA. Translation: BAB12769.1 .
RefSeqi NP_239883.1. NC_002528.1.

3D structure databases

ProteinModelPortali P57154.
SMRi P57154. Positions 8-292.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAB12769 ; BAB12769 ; BAB12769 .
GeneIDi 1109550.
KEGGi buc:BU046.
PATRICi 21243598. VBIBucAph127364_0055.

Phylogenomic databases

eggNOGi COG0685.
HOGENOMi HOG000246232.
KOi K00297.
OMAi FEFFPTK.
OrthoDBi EOG6D2KVW.

Enzyme and pathway databases

UniPathwayi UPA00193 .
BioCyci BAPH107806:GBZJ-46-MONOMER.

Family and domain databases

Gene3Di 3.20.20.220. 1 hit.
InterProi IPR029041. FAD-linked_oxidoreductase-like.
IPR003171. Mehydrof_redctse.
IPR004620. MTHF_reductase_bac.
[Graphical view ]
Pfami PF02219. MTHFR. 1 hit.
[Graphical view ]
SUPFAMi SSF51730. SSF51730. 1 hit.
TIGRFAMsi TIGR00676. fadh2. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
    Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
    Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: APS.

Entry informationi

Entry nameiMETF_BUCAI
AccessioniPrimary (citable) accession number: P57154
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: June 11, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Buchnera aphidicola (subsp. Acyrthosiphon pisum)
    Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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