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P57154

- METF_BUCAI

UniProt

P57154 - METF_BUCAI

Protein

5,10-methylenetetrahydrofolate reductase

Gene

metF

Organism
Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 79 (01 Oct 2014)
      Sequence version 1 (01 Dec 2000)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H.

    Cofactori

    FAD.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei28 – 281Proton donor/acceptorBy similarity
    Binding sitei88 – 881FADBy similarity
    Binding sitei120 – 1201SubstrateBy similarity
    Binding sitei152 – 1521FADBy similarity
    Binding sitei183 – 1831SubstrateBy similarity
    Binding sitei223 – 2231SubstrateBy similarity
    Binding sitei275 – 2751SubstrateBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi28 – 336NADBy similarity
    Nucleotide bindingi59 – 624FADBy similarity
    Nucleotide bindingi59 – 602NADBy similarity
    Nucleotide bindingi118 – 1203FADBy similarity
    Nucleotide bindingi131 – 1322FADBy similarity
    Nucleotide bindingi156 – 1594FADBy similarity
    Nucleotide bindingi165 – 1728FADBy similarity

    GO - Molecular functioni

    1. methylenetetrahydrofolate reductase (NAD(P)H) activity Source: UniProtKB-EC

    GO - Biological processi

    1. methionine biosynthetic process Source: UniProtKB-KW
    2. tetrahydrofolate interconversion Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Amino-acid biosynthesis, Methionine biosynthesis

    Keywords - Ligandi

    FAD, Flavoprotein, NAD, NADP

    Enzyme and pathway databases

    BioCyciBAPH107806:GBZJ-46-MONOMER.
    UniPathwayiUPA00193.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    5,10-methylenetetrahydrofolate reductase (EC:1.5.1.20)
    Gene namesi
    Name:metF
    Ordered Locus Names:BU046
    OrganismiBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
    Taxonomic identifieri107806 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
    ProteomesiUP000001806: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 2922925,10-methylenetetrahydrofolate reductasePRO_0000190258Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliP57154.
    SMRiP57154. Positions 8-292.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0685.
    HOGENOMiHOG000246232.
    KOiK00297.
    OMAiFEFFPTK.
    OrthoDBiEOG6D2KVW.

    Family and domain databases

    Gene3Di3.20.20.220. 1 hit.
    InterProiIPR029041. FAD-linked_oxidoreductase-like.
    IPR003171. Mehydrof_redctse.
    IPR004620. MTHF_reductase_bac.
    [Graphical view]
    PfamiPF02219. MTHFR. 1 hit.
    [Graphical view]
    SUPFAMiSSF51730. SSF51730. 1 hit.
    TIGRFAMsiTIGR00676. fadh2. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P57154-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKNYSQYHQD IMNQKLENIR NNIQCSFEFF PPKNSFLEEN LWSTVNRLSL    50
    LKPKFFSVTY GANTGEREKT YDTVQKIYKK TGIITAAHLT CIDSTPHKLK 100
    EIAYNYWNSG IKSIVALRGD TLEKDYRHTM YAVDLVLLLK KIADFDISVA 150
    AYPELHPESK NVKSDILNLK KKVDAGASRA ITQFFFNIES YLRFRDNCIK 200
    NKINIDIIPG ILPVCNFQKL KRFSSMTNVK IPKWMLDMFN GLDDDIFTQK 250
    IIGSSIAIDM VKKLSCEGVK NFHFYTLNQS DITYSICHIL GL 292
    Length:292
    Mass (Da):33,730
    Last modified:December 1, 2000 - v1
    Checksum:i1D2A9E1B4D6E0DA9
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000003 Genomic DNA. Translation: BAB12769.1.
    RefSeqiNP_239883.1. NC_002528.1.

    Genome annotation databases

    EnsemblBacteriaiBAB12769; BAB12769; BAB12769.
    GeneIDi1109550.
    KEGGibuc:BU046.
    PATRICi21243598. VBIBucAph127364_0055.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000003 Genomic DNA. Translation: BAB12769.1 .
    RefSeqi NP_239883.1. NC_002528.1.

    3D structure databases

    ProteinModelPortali P57154.
    SMRi P57154. Positions 8-292.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAB12769 ; BAB12769 ; BAB12769 .
    GeneIDi 1109550.
    KEGGi buc:BU046.
    PATRICi 21243598. VBIBucAph127364_0055.

    Phylogenomic databases

    eggNOGi COG0685.
    HOGENOMi HOG000246232.
    KOi K00297.
    OMAi FEFFPTK.
    OrthoDBi EOG6D2KVW.

    Enzyme and pathway databases

    UniPathwayi UPA00193 .
    BioCyci BAPH107806:GBZJ-46-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.220. 1 hit.
    InterProi IPR029041. FAD-linked_oxidoreductase-like.
    IPR003171. Mehydrof_redctse.
    IPR004620. MTHF_reductase_bac.
    [Graphical view ]
    Pfami PF02219. MTHFR. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51730. SSF51730. 1 hit.
    TIGRFAMsi TIGR00676. fadh2. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
      Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
      Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: APS.

    Entry informationi

    Entry nameiMETF_BUCAI
    AccessioniPrimary (citable) accession number: P57154
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: December 1, 2000
    Last modified: October 1, 2014
    This is version 79 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Buchnera aphidicola (subsp. Acyrthosiphon pisum)
      Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3