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P57154 (METF_BUCAI) Reviewed, UniProtKB/Swiss-Prot

Last modified December 11, 2013. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
5,10-methylenetetrahydrofolate reductase

EC=1.5.1.20
Gene names
Name:metF
Ordered Locus Names:BU046
OrganismBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium) [Reference proteome] [HAMAP]
Taxonomic identifier107806 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera

Protein attributes

Sequence length292 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H.

Cofactor

FAD By similarity.

Pathway

One-carbon metabolism; tetrahydrofolate interconversion.

Sequence similarities

Belongs to the methylenetetrahydrofolate reductase family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 2922925,10-methylenetetrahydrofolate reductase
PRO_0000190258

Regions

Nucleotide binding28 – 336NAD By similarity
Nucleotide binding59 – 624FAD By similarity
Nucleotide binding59 – 602NAD By similarity
Nucleotide binding118 – 1203FAD By similarity
Nucleotide binding131 – 1322FAD By similarity
Nucleotide binding156 – 1594FAD By similarity
Nucleotide binding165 – 1728FAD By similarity

Sites

Active site281Proton donor/acceptor By similarity
Binding site881FAD By similarity
Binding site1201Substrate By similarity
Binding site1521FAD By similarity
Binding site1831Substrate By similarity
Binding site2231Substrate By similarity
Binding site2751Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
P57154 [UniParc].

Last modified December 1, 2000. Version 1.
Checksum: 1D2A9E1B4D6E0DA9

FASTA29233,730
        10         20         30         40         50         60 
MKNYSQYHQD IMNQKLENIR NNIQCSFEFF PPKNSFLEEN LWSTVNRLSL LKPKFFSVTY 

        70         80         90        100        110        120 
GANTGEREKT YDTVQKIYKK TGIITAAHLT CIDSTPHKLK EIAYNYWNSG IKSIVALRGD 

       130        140        150        160        170        180 
TLEKDYRHTM YAVDLVLLLK KIADFDISVA AYPELHPESK NVKSDILNLK KKVDAGASRA 

       190        200        210        220        230        240 
ITQFFFNIES YLRFRDNCIK NKINIDIIPG ILPVCNFQKL KRFSSMTNVK IPKWMLDMFN 

       250        260        270        280        290 
GLDDDIFTQK IIGSSIAIDM VKKLSCEGVK NFHFYTLNQS DITYSICHIL GL 

« Hide

References

[1]"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: APS.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000003 Genomic DNA. Translation: BAB12769.1.
RefSeqNP_239883.1. NC_002528.1.

3D structure databases

ProteinModelPortalP57154.
SMRP57154. Positions 8-292.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAB12769; BAB12769; BAB12769.
GeneID1109550.
KEGGbuc:BU046.
PATRIC21243598. VBIBucAph127364_0055.

Phylogenomic databases

eggNOGCOG0685.
HOGENOMHOG000246232.
KOK00297.
OMAFIRAETG.
OrthoDBEOG6D2KVW.
ProtClustDBPRK09432.

Enzyme and pathway databases

BioCycBAPH107806:GBZJ-46-MONOMER.
UniPathwayUPA00193.

Family and domain databases

InterProIPR003171. Mehydrof_redctse.
IPR004620. MTHF_reductase_bac.
[Graphical view]
PfamPF02219. MTHFR. 1 hit.
[Graphical view]
TIGRFAMsTIGR00676. fadh2. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMETF_BUCAI
AccessionPrimary (citable) accession number: P57154
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: December 11, 2013
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Buchnera aphidicola (subsp. Acyrthosiphon pisum)

Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names