Reviewed, UniProtKB/Swiss-Prot P57154 (METF_BUCAI)

Names and origin

Protein names

Recommended name:
    5,10-methylenetetrahydrofolate reductase
    EC=1.5.1.20

Gene names

Name:	metF
Ordered Locus Names:	BU046

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (Acyrthosiphon pisum symbiotic bacterium) [Complete proteome] [HAMAP]

Taxonomic identifier

118099 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	292 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	5-methyltetrahydrofolate + NAD(P)(+) = 5,10-methylenetetrahydrofolate + NAD(P)H.
Cofactor	FAD By similarity.
Pathway	One-carbon metabolism; tetrahydrofolate pathway.
Sequence similarities	Belongs to the methylenetetrahydrofolate reductase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Ligand	FAD Flavoprotein NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	methylenetetrahydrofolate reductase (NADPH) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 292	292	5,10-methylenetetrahydrofolate reductase		PRO_0000190258

Sequences

	Sequence		Length	Mass (Da)
	P57154-1 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: 1D2A9E1B4D6E0DA9	FASTA	292	33,730
	10 20 30 40 50 60 MKNYSQYHQD IMNQKLENIR NNIQCSFEFF PPKNSFLEEN LWSTVNRLSL LKPKFFSVTY 70 80 90 100 110 120 GANTGEREKT YDTVQKIYKK TGIITAAHLT CIDSTPHKLK EIAYNYWNSG IKSIVALRGD 130 140 150 160 170 180 TLEKDYRHTM YAVDLVLLLK KIADFDISVA AYPELHPESK NVKSDILNLK KKVDAGASRA 190 200 210 220 230 240 ITQFFFNIES YLRFRDNCIK NKINIDIIPG ILPVCNFQKL KRFSSMTNVK IPKWMLDMFN 250 260 270 280 290 GLDDDIFTQK IIGSSIAIDM VKKLSCEGVK NFHFYTLNQS DITYSICHIL GL

References

[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed: 10993077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tokyo 1998.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000003 Genomic DNA. Translation: BAB12769.1.
RefSeq	NP_239883.1.
3D structure databases
HSSP	HSSP built from PDB template 1B5T based on UniProtKB P00394.
ModBase	Search...
Genome annotation databases
GeneID	1109550.
GenomeReviews	Gene locus BU046 in contig BA000003_GR.
KEGG	buc:BU046.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P57154.
Enzyme and pathway databases
BioCyc	BSP107806:BU046-MON.
Family and domain databases
InterPro	IPR003171. Mehydrof_redctse. IPR004620. MTHF_reductase_bac. [Graphical view]
Pfam	PF02219. MTHFR. 1 hit. [Graphical view]
TIGRFAMs	TIGR00676. fadh2. 1 hit.
ProtoNet	Search...

Entry information

Entry name

METF_BUCAI

Accession

Primary (citable) accession number: P57154

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	November 25, 2008
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)