Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

5,10-methylenetetrahydrofolate reductase

Gene

metF

Organism
Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H.

Cofactori

FADBy similarity

Pathwayi: tetrahydrofolate interconversion

This protein is involved in the pathway tetrahydrofolate interconversion, which is part of One-carbon metabolism.
View all proteins of this organism that are known to be involved in the pathway tetrahydrofolate interconversion and in One-carbon metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei28Proton donor/acceptorBy similarity1
Binding sitei88FADBy similarity1
Binding sitei120SubstrateBy similarity1
Binding sitei152FADBy similarity1
Binding sitei183SubstrateBy similarity1
Binding sitei223SubstrateBy similarity1
Binding sitei275SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi28 – 33NADBy similarity6
Nucleotide bindingi59 – 62FADBy similarity4
Nucleotide bindingi59 – 60NADBy similarity2
Nucleotide bindingi118 – 120FADBy similarity3
Nucleotide bindingi131 – 132FADBy similarity2
Nucleotide bindingi156 – 159FADBy similarity4
Nucleotide bindingi165 – 172FADBy similarity8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Methionine biosynthesis

Keywords - Ligandi

FAD, Flavoprotein, NAD, NADP

Enzyme and pathway databases

UniPathwayiUPA00193.

Names & Taxonomyi

Protein namesi
Recommended name:
5,10-methylenetetrahydrofolate reductase (EC:1.5.1.20)
Gene namesi
Name:metF
Ordered Locus Names:BU046
OrganismiBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Taxonomic identifieri107806 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesErwiniaceaeBuchnera
Proteomesi
  • UP000001806 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001902581 – 2925,10-methylenetetrahydrofolate reductaseAdd BLAST292

Interactioni

Protein-protein interaction databases

STRINGi107806.BU046.

Structurei

3D structure databases

ProteinModelPortaliP57154.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105SYT. Bacteria.
COG0685. LUCA.
HOGENOMiHOG000246232.
KOiK00297.
OMAiEMHPQAR.

Family and domain databases

CDDicd00537. MTHFR. 1 hit.
Gene3Di3.20.20.220. 1 hit.
InterProiIPR029041. FAD-linked_oxidoreductase-like.
IPR003171. Mehydrof_redctse.
IPR004620. MTHF_reductase_bac.
[Graphical view]
PfamiPF02219. MTHFR. 1 hit.
[Graphical view]
SUPFAMiSSF51730. SSF51730. 1 hit.
TIGRFAMsiTIGR00676. fadh2. 1 hit.

Sequencei

Sequence statusi: Complete.

P57154-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKNYSQYHQD IMNQKLENIR NNIQCSFEFF PPKNSFLEEN LWSTVNRLSL
60 70 80 90 100
LKPKFFSVTY GANTGEREKT YDTVQKIYKK TGIITAAHLT CIDSTPHKLK
110 120 130 140 150
EIAYNYWNSG IKSIVALRGD TLEKDYRHTM YAVDLVLLLK KIADFDISVA
160 170 180 190 200
AYPELHPESK NVKSDILNLK KKVDAGASRA ITQFFFNIES YLRFRDNCIK
210 220 230 240 250
NKINIDIIPG ILPVCNFQKL KRFSSMTNVK IPKWMLDMFN GLDDDIFTQK
260 270 280 290
IIGSSIAIDM VKKLSCEGVK NFHFYTLNQS DITYSICHIL GL
Length:292
Mass (Da):33,730
Last modified:December 1, 2000 - v1
Checksum:i1D2A9E1B4D6E0DA9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000003 Genomic DNA. Translation: BAB12769.1.
RefSeqiNP_239883.1. NC_002528.1.
WP_010895916.1. NC_002528.1.

Genome annotation databases

EnsemblBacteriaiBAB12769; BAB12769; BAB12769.
GeneIDi1109550.
KEGGibuc:BU046.
PATRICi21243598. VBIBucAph127364_0055.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000003 Genomic DNA. Translation: BAB12769.1.
RefSeqiNP_239883.1. NC_002528.1.
WP_010895916.1. NC_002528.1.

3D structure databases

ProteinModelPortaliP57154.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi107806.BU046.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB12769; BAB12769; BAB12769.
GeneIDi1109550.
KEGGibuc:BU046.
PATRICi21243598. VBIBucAph127364_0055.

Phylogenomic databases

eggNOGiENOG4105SYT. Bacteria.
COG0685. LUCA.
HOGENOMiHOG000246232.
KOiK00297.
OMAiEMHPQAR.

Enzyme and pathway databases

UniPathwayiUPA00193.

Family and domain databases

CDDicd00537. MTHFR. 1 hit.
Gene3Di3.20.20.220. 1 hit.
InterProiIPR029041. FAD-linked_oxidoreductase-like.
IPR003171. Mehydrof_redctse.
IPR004620. MTHF_reductase_bac.
[Graphical view]
PfamiPF02219. MTHFR. 1 hit.
[Graphical view]
SUPFAMiSSF51730. SSF51730. 1 hit.
TIGRFAMsiTIGR00676. fadh2. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMETF_BUCAI
AccessioniPrimary (citable) accession number: P57154
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: November 2, 2016
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Buchnera aphidicola (subsp. Acyrthosiphon pisum)
    Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.