P57153 (MURB_BUCAI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-N-acetylenolpyruvoylglucosamine reductase
EC=1.3.1.98

Alternative name(s):
UDP-N-acetylmuramate dehydrogenase

Gene names

Name:	murB
Ordered Locus Names:	BU045

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium) [Reference proteome] [HAMAP]

Taxonomic identifier

107806 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera › Buchnera aphidicola › Buchnera aphidicola (Acyrthosiphon pisum)

Protein attributes

Sequence length	356 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cell wall formation By similarity.
Catalytic activity	UDP-N-acetyl-alpha-D-muramate + NADP+ = UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine + NADPH. HAMAP-Rule MF_00037
Cofactor	FAD By similarity.
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00037
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the MurB family. Contains 1 FAD-binding PCMH-type domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	UDP-N-acetylmuramate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 356	356	UDP-N-acetylenolpyruvoylglucosamine reductase HAMAP-Rule MF_00037		PRO_0000179187
Regions
	Domain	20 – 190	171	FAD-binding PCMH-type
Sites
	Active site	166	1	By similarity
	Active site	236	1	Proton donor By similarity
	Active site	331	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P57153 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: 103486BF1DE25EA7	FASTA	356	40,593
	10 20 30 40 50 60 MHKKKYFSSQ SLKDLNTFAI DVTAKKIIFV KTIQSLIDIS KICQLSNIPY IILGEGSNVL 70 80 90 100 110 120 FLENYVGVVI INRIKGIKIV EKKNFWLLHV FSGEKWHNLV KLTLNLGILG LENLALIPGC 130 140 150 160 170 180 IGSAAIQNIG AYGLEFKDVC QYVDILSLKD NTIKRIYKNS CKFSYRNSIF KDQYKNEHAV 190 200 210 220 230 240 IRVGIKLSKK WRPILFSSLE NYITPINITA YKIFNIICKI RKKKLPDPKK IGNAGSFFKN 250 260 270 280 290 300 PLIKKKKAQK LINLYKVPNY PQKNGLVKIS AAWLIENYKF KHLQIGDAAI HKKQKLILIN 310 320 330 340 350 KKNATAQEII KLAKIIHKCI LKKFNILLEP EVDLIGASGK IKASKIFKLN SKLKVI

References

[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: APS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000003 Genomic DNA. Translation: BAB12768.1.
RefSeq	NP_239882.1. NC_002528.1.
3D structure databases
ProteinModelPortal	P57153.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAB12768; BAB12768; BAB12768.
GeneID	1109549.
KEGG	buc:BU045.
PATRIC	21243596. VBIBucAph127364_0054.
Phylogenomic databases
eggNOG	COG0812.
HOGENOM	HOG000284356.
KO	K00075.
OMA	RDSVFKH.
ProtClustDB	PRK00046.
Enzyme and pathway databases
UniPathway	UPA00219.
Family and domain databases
Gene3D	3.30.43.10. 1 hit. 3.30.465.10. 1 hit. 3.90.78.10. 1 hit.
HAMAP	MF_00037. MurB.
InterPro	IPR016169. CO_DH_flavot_FAD-bd_sub2. IPR016166. FAD-bd_2. IPR016167. FAD-bd_2_sub1. IPR003170. MurB. IPR011601. MurB_C. IPR006094. Oxid_FAD_bind_N. [Graphical view]
PANTHER	PTHR21071. PTHR21071. 1 hit.
Pfam	PF01565. FAD_binding_4. 1 hit. PF02873. MurB_C. 1 hit. [Graphical view]
SUPFAM	SSF56176. FAD-binding_2. 1 hit. SSF56194. MurB_C. 1 hit.
TIGRFAMs	TIGR00179. murB. 1 hit.
PROSITE	PS51387. FAD_PCMH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MURB_BUCAI

Accession

Primary (citable) accession number: P57153

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	May 1, 2013
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program