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Protein

UDP-N-acetylenolpyruvoylglucosamine reductase

Gene

murB

Organism
Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Cell wall formation.By similarity

Catalytic activityi

UDP-N-acetyl-alpha-D-muramate + NADP+ = UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine + NADPH.

Cofactori

FADBy similarity

Pathway:ipeptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei166 – 1661By similarity
Active sitei236 – 2361Proton donorBy similarity
Active sitei331 – 3311By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Cell cycle, Cell division, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Keywords - Ligandi

FAD, Flavoprotein, NADP

Enzyme and pathway databases

BioCyciBAPH107806:GBZJ-45-MONOMER.
UniPathwayiUPA00219.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-N-acetylenolpyruvoylglucosamine reductase (EC:1.3.1.98)
Alternative name(s):
UDP-N-acetylmuramate dehydrogenase
Gene namesi
Name:murB
Ordered Locus Names:BU045
OrganismiBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Taxonomic identifieri107806 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
ProteomesiUP000001806 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 356356UDP-N-acetylenolpyruvoylglucosamine reductasePRO_0000179187Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi107806.BU045.

Structurei

3D structure databases

ProteinModelPortaliP57153.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini20 – 190171FAD-binding PCMH-typeAdd
BLAST

Sequence similaritiesi

Belongs to the MurB family.Curated
Contains 1 FAD-binding PCMH-type domain.Curated

Phylogenomic databases

eggNOGiCOG0812.
HOGENOMiHOG000284356.
KOiK00075.
OMAiMQNIGAY.
OrthoDBiEOG60CWQ3.

Family and domain databases

Gene3Di3.30.43.10. 1 hit.
3.30.465.10. 1 hit.
3.90.78.10. 1 hit.
HAMAPiMF_00037. MurB.
InterProiIPR016169. CO_DH_flavot_FAD-bd_sub2.
IPR016166. FAD-bd_2.
IPR016167. FAD-bd_2_sub1.
IPR003170. MurB.
IPR011601. MurB_C.
IPR006094. Oxid_FAD_bind_N.
[Graphical view]
PANTHERiPTHR21071. PTHR21071. 1 hit.
PfamiPF01565. FAD_binding_4. 1 hit.
PF02873. MurB_C. 1 hit.
[Graphical view]
SUPFAMiSSF56176. SSF56176. 1 hit.
SSF56194. SSF56194. 1 hit.
TIGRFAMsiTIGR00179. murB. 1 hit.
PROSITEiPS51387. FAD_PCMH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P57153-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHKKKYFSSQ SLKDLNTFAI DVTAKKIIFV KTIQSLIDIS KICQLSNIPY
60 70 80 90 100
IILGEGSNVL FLENYVGVVI INRIKGIKIV EKKNFWLLHV FSGEKWHNLV
110 120 130 140 150
KLTLNLGILG LENLALIPGC IGSAAIQNIG AYGLEFKDVC QYVDILSLKD
160 170 180 190 200
NTIKRIYKNS CKFSYRNSIF KDQYKNEHAV IRVGIKLSKK WRPILFSSLE
210 220 230 240 250
NYITPINITA YKIFNIICKI RKKKLPDPKK IGNAGSFFKN PLIKKKKAQK
260 270 280 290 300
LINLYKVPNY PQKNGLVKIS AAWLIENYKF KHLQIGDAAI HKKQKLILIN
310 320 330 340 350
KKNATAQEII KLAKIIHKCI LKKFNILLEP EVDLIGASGK IKASKIFKLN

SKLKVI
Length:356
Mass (Da):40,593
Last modified:December 1, 2000 - v1
Checksum:i103486BF1DE25EA7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000003 Genomic DNA. Translation: BAB12768.1.
RefSeqiNP_239882.1. NC_002528.1.
WP_010895915.1. NC_002528.1.

Genome annotation databases

EnsemblBacteriaiBAB12768; BAB12768; BAB12768.
GeneIDi1109549.
KEGGibuc:BU045.
PATRICi21243596. VBIBucAph127364_0054.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000003 Genomic DNA. Translation: BAB12768.1.
RefSeqiNP_239882.1. NC_002528.1.
WP_010895915.1. NC_002528.1.

3D structure databases

ProteinModelPortaliP57153.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi107806.BU045.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB12768; BAB12768; BAB12768.
GeneIDi1109549.
KEGGibuc:BU045.
PATRICi21243596. VBIBucAph127364_0054.

Phylogenomic databases

eggNOGiCOG0812.
HOGENOMiHOG000284356.
KOiK00075.
OMAiMQNIGAY.
OrthoDBiEOG60CWQ3.

Enzyme and pathway databases

UniPathwayiUPA00219.
BioCyciBAPH107806:GBZJ-45-MONOMER.

Family and domain databases

Gene3Di3.30.43.10. 1 hit.
3.30.465.10. 1 hit.
3.90.78.10. 1 hit.
HAMAPiMF_00037. MurB.
InterProiIPR016169. CO_DH_flavot_FAD-bd_sub2.
IPR016166. FAD-bd_2.
IPR016167. FAD-bd_2_sub1.
IPR003170. MurB.
IPR011601. MurB_C.
IPR006094. Oxid_FAD_bind_N.
[Graphical view]
PANTHERiPTHR21071. PTHR21071. 1 hit.
PfamiPF01565. FAD_binding_4. 1 hit.
PF02873. MurB_C. 1 hit.
[Graphical view]
SUPFAMiSSF56176. SSF56176. 1 hit.
SSF56194. SSF56194. 1 hit.
TIGRFAMsiTIGR00179. murB. 1 hit.
PROSITEiPS51387. FAD_PCMH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
    Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
    Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: APS.

Entry informationi

Entry nameiMURB_BUCAI
AccessioniPrimary (citable) accession number: P57153
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: July 22, 2015
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Buchnera aphidicola (subsp. Acyrthosiphon pisum)
    Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.