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P57146 (RPOB_BUCAI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA-directed RNA polymerase subunit beta
Short name=RNAP subunit beta
EC=2.7.7.6
Alternative name(s):
RNA polymerase subunit beta
Transcriptase subunit beta
Gene names
Name:rpoB
Ordered Locus Names:BU034
OrganismBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium) [Reference proteome] [HAMAP]
Taxonomic identifier107806 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera ›

Protein attributes

Sequence length1342 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates By similarity. HAMAP-Rule MF_01321

Catalytic activity

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). HAMAP-Rule MF_01321

Subunit structure

The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription By similarity.

Sequence similarities

Belongs to the RNA polymerase beta chain family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 13421342DNA-directed RNA polymerase subunit beta HAMAP-Rule MF_01321
PRO_0000047872

Sequences

Sequence LengthMass (Da)Tools
P57146 [UniParc].

Last modified December 1, 2000. Version 1.
Checksum: 95252459873DF940

FASTA1,342151,468
        10         20         30         40         50         60 
MVYSYTEKKR IRKDFGKRPK VLDIPYLLSI QLNSFKKFIQ PDLSGQHGLE AAFRSVFPIR 

        70         80         90        100        110        120 
GYNGNSELQY VSYRLGETIF DVKECQIRGA TYSAALRVKL RLVIYERDIL EATVKDIKEQ 

       130        140        150        160        170        180 
EVYMGEIPLM TNNGTFIING TERVVVSQLH RSPGVFFDSD KGKTHSSGKV LYNARIIPYR 

       190        200        210        220        230        240 
GSWLDFEFDP KDNLFVRIDR RRKLPVSIIL RALNYNTEEI LNIFFEKNIF KIENNKIVLE 

       250        260        270        280        290        300 
LVPERLRGET ASFNIKKNGI IYVEKGRRIT AKHIQELKNN KIKSITVPVE YILGRIVSKN 

       310        320        330        340        350        360 
YVDKKTGETI ISSNTELSLE ILEKLKKLGF YSIETLFTND LDHGPYISET LRIDSSNDRM 

       370        380        390        400        410        420 
SALIEIYRVM RPGEPPTKEA TENLFENLFF SEDRYDLSTV GRMKFNRSLL REETKGSSTL 

       430        440        450        460        470        480 
NKEDIIDVIK KIIDIRNGKG EVDDIDHLGN RRVRSVGEMA ENQFRIGLVR VERAVKERLS 

       490        500        510        520        530        540 
IGDLDALMPQ DMINAKPISA AVKEFFGSSQ LSQFMDQNNP LSEITHKRRI SALGLGGLTR 

       550        560        570        580        590        600 
ERAGFEVRDV HPTHYGRVCP IETPEGPNIG LINSLSVYAQ TNSHGFLETP YRKVCNGLVS 

       610        620        630        640        650        660 
EEIHYLSAIE EGNYIIAQAN TNIDKTGFFT DDLVTCRHKG ESSLFNRNQV NYMDVSTQQI 

       670        680        690        700        710        720 
VSVGASLIPF PEHDDANRAL MGANMQRQAV PTLKTDKPLI GTGMERAVAV DSGVTAVAKR 

       730        740        750        760        770        780 
SGVVQYVDAS RIVIKVDEKE MYSREAGIDI YNLTKYTRSN QNTCINQQPC VNLGEKIKKG 

       790        800        810        820        830        840 
DVLADGPSTD LGELALGQNM RVAFMPWNGY NFEDSILVSE RVVQEDRFTT IHIQELSCIS 

       850        860        870        880        890        900 
RDTKLGAEEI SSDIPNVGEA ALSKLDESGI VYIGAEVTGG DILVGKVTPK GETQLTPEEK 

       910        920        930        940        950        960 
LLRAIFGEKA SDVKDSSLRV PNGVSGTVID VQIFTRDGVK KDKRALEIED MQLKKIKQDL 

       970        980        990       1000       1010       1020 
TEEFKIFESS LFTHIKKTFI SLDIETKQLD TLPFEKWFSV DIKQKDKKKE IEKLAKQHHE 

      1030       1040       1050       1060       1070       1080 
LKEEFNKKIE IKRQKITQGD DLAPGVLKIV KVYLAVKRQI QPGDKMAGRH GNKGVISKIN 

      1090       1100       1110       1120       1130       1140 
PVEDMPYDEN GIPVDIVLNP LGVPSRMNIG QILETHLGMA AKGIGDKINH MLKTQEKISN 

      1150       1160       1170       1180       1190       1200 
LRKFIQKAFD LGDNLRQKVN LDTFSNEEIL RLAKNLKNGI PISTPVFDGA QENEIKQMLK 

      1210       1220       1230       1240       1250       1260 
FAGLPTSGQI ILFDGRTGEK FERPVTVGYM YMLKLNHLVD DKMHARSTGS YSLVTQQPLG 

      1270       1280       1290       1300       1310       1320 
GKAQFGGQRF GEMEVWALEA YGASYTLQEM LTVKSDDVNG RTKMYKNIVD GNHQMEPGMP 

      1330       1340 
ESFNVLLKEI RSLGINIELE SE

« Hide

References

[1]"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: APS.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BA000003 Genomic DNA. Translation: BAB12761.1.
RefSeqNP_239875.1. NC_002528.1.

3D structure databases

ProteinModelPortalP57146.
ModBaseSearch...

Proteomic databases

PRIDEP57146.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAB12761; BAB12761; BAB12761.
GeneID1109542.
KEGGbuc:BU034.
PATRIC21243574. VBIBucAph127364_0047.

Phylogenomic databases

eggNOGCOG0085.
HOGENOMHOG000218612.
KOK03043.
OMAFMTWEGY.
ProtClustDBPRK00405.

Family and domain databases

Gene3D2.30.150.10. 1 hit.
2.40.270.10. 2 hits.
2.40.50.150. 2 hits.
HAMAPMF_01321. RNApol_bact_RpoB.
InterProIPR010243. DNA-dir_RNA_pol_bsu.
IPR019462. DNA-dir_RNA_pol_bsu_external_1.
IPR015712. DNA-dir_RNA_pol_su2.
IPR007120. DNA-dir_RNA_pol_su2_6.
IPR007121. RNA_pol_bsu_CS.
IPR007644. RNA_pol_bsu_protrusion.
IPR007642. RNA_pol_Rpb2_2.
IPR007645. RNA_pol_Rpb2_3.
IPR007641. RNA_pol_Rpb2_7.
IPR014724. RNA_pol_RPB2_OB-fold.
[Graphical view]
PANTHERPTHR20856. PTHR20856. 1 hit.
PTHR20856:SF3. PTHR20856:SF3. 1 hit.
PfamPF04563. RNA_pol_Rpb2_1. 1 hit.
PF04561. RNA_pol_Rpb2_2. 2 hits.
PF04565. RNA_pol_Rpb2_3. 1 hit.
PF10385. RNA_pol_Rpb2_45. 1 hit.
PF00562. RNA_pol_Rpb2_6. 1 hit.
PF04560. RNA_pol_Rpb2_7. 1 hit.
[Graphical view]
TIGRFAMsTIGR02013. rpoB. 1 hit.
PROSITEPS01166. RNA_POL_BETA. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRPOB_BUCAI
AccessionPrimary (citable) accession number: P57146
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: May 1, 2013
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Buchnera aphidicola (subsp. Acyrthosiphon pisum)

Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names

SIMILARITY comments

Index of protein domains and families

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