P57145 (RPOC_BUCAI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA-directed RNA polymerase subunit beta'
Short name=RNAP subunit beta'
EC=2.7.7.6

Alternative name(s):
RNA polymerase subunit beta'
Transcriptase subunit beta'

Gene names

Name:	rpoC
Ordered Locus Names:	BU033

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)

Taxonomic identifier

107806 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	1407 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. HAMAP MF_01322
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). HAMAP MF_01322
Subunit structure	The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription By similarity.
Sequence similarities	Belongs to the RNA polymerase beta' chain family.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW coenzyme binding Inferred from electronic annotation. Source: InterPro oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1407	1407	DNA-directed RNA polymerase subunit beta' HAMAP MF_01322		PRO_0000067720

Sequences

	Sequence		Length	Mass (Da)
	P57145 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: 4BCD02B8F53A4BAF	FASTA	1,407	156,944
	10 20 30 40 50 60 MKDLLKFLKS QTKNEDFDAI KISLASPDMI RSWSFGEVKK PETINYRTFK PERDGLFCAR 70 80 90 100 110 120 IFGPVKDYEC LCGKYKRLKH RGVICEKCGV EVTQSKVRRE RMGHIELSSP TAHIWFLKSL 130 140 150 160 170 180 PSRIGLLLDM PLRDIERVLY FESYVVIETG MTNLEKRQIL TEEQYLDSLE EFGDEFHATM 190 200 210 220 230 240 GAEAIQFLLK DINLVQECNV LRIELNETNS ETKRKKLTKR IKLLESFIQS HNKPEWMILN 250 260 270 280 290 300 VLPVLPPDLR PLVPLDGGRF ATSDLNDLYR RVINRNNRLK RLLDLAAPDI IVRNEKRMLQ 310 320 330 340 350 360 EAVDALLDNG RRGRAITGSN KRPLKSLADM IKGKQGRFRQ NLLGKRVDYS GRSVITVGPY 370 380 390 400 410 420 LHLHQCGLPK KMALELFKPF IYGKLEVRGL ATTIKAAKKM VEREEAIVWD ILDEVIREHP 430 440 450 460 470 480 VLLNRAPTLH RLGIQAFEPV LIEGKAIQLH PLVCAAYNAD FDGDQMAVHV PLTLESQLEA 490 500 510 520 530 540 RALMMSTNNI LSPANGEPII VPSQDVVLGL YYMTREKING KGEGMLLNGS NEAEKVYRLG 550 560 570 580 590 600 IAELHSLVKV RIIEYKKNED KSFTAIKKII PTTIGRAILW MIIPKGLPFS IVNQTLGKKD 610 620 630 640 650 660 ISKMLNTCYR ILGLKSTVFF ADQIMYTGFA YAARSGASVG IDDMVIPEKK ANIINEAEIE 670 680 690 700 710 720 VAEIQEQFQS GLVTAGERYN KVIDIWAAAN ERVAKAMMQN LSTESVINKK GYKQKQISFN 730 740 750 760 770 780 SIFMMADSGA RGSAAQIRQL AGMRGLMAKP DGSIIETPIT ANFREGLNVL QYFISTHGAR 790 800 810 820 830 840 KGLADTALKT ANSGYLTRRL VDVAQDLVVT QDDCRTHEGI LMTPLIEGGD VKEPLRERVL 850 860 870 880 890 900 GRVTAENIII PNTKNILIKR NTLLNEKWCD LLEHNSIDNV KVRSVVNCDT DFGVCAYCYG 910 920 930 940 950 960 RDLARGNLVN KGEAIGVIAA QSIGEPGTQL TMRTFHIGGA ASRAAAESSI QVKNQGIINL 970 980 990 1000 1010 1020 NNAKFVINSA GKTVITSRNV ELNIIDNFGR TKESYKVPYG AIMAKGDGEK VHSGETVAKW 1030 1040 1050 1060 1070 1080 DPHTMPVITE VNGLVRFVDM IDGQSITRQA DELTGLSSIV ILDTAERMSS GKDLRPALKI 1090 1100 1110 1120 1130 1140 IDCNGNDVLI SGTDMPAQYF LPGKAIVQLD DGVQISSGDT LARVPQESGG TKDITGGLPR 1150 1160 1170 1180 1190 1200 VADLFEARRP KELAILAEIS GIISFGKETK GKRRLVITPV DGSDSYEEMI PKWRQLNVFE 1210 1220 1230 1240 1250 1260 GERVDRGDVI SDGPESPHDI LRLRGVQAVT RYIVNEVQEV YRLQGVKIND KHIEVIIRQM 1270 1280 1290 1300 1310 1320 LRKATVVKSR DSDFLEGEQV EFSHIKISNR MLDKKKKMPA TFSRDLLGIT KASLATESFI 1330 1340 1350 1360 1370 1380 SAASFQETTR VLTESAVAGK RDELRGLKEN VIVGRLIPAG TGYAYHKERL NRRQKKHNNP 1390 1400 TVSSSQISAE EASASLSELL NSALIEK

References

[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed: 10993077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: APS.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000003 Genomic DNA. Translation: BAB12760.1.
RefSeq	NP_239874.1. NC_002528.1.
3D structure databases
ProteinModelPortal	P57145.
SMR	P57145. Positions 20-165, 944-1128.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBUCT00000002865; EBBUCP00000002641; EBBUCG00000002865.
GeneID	1109541.
GenomeReviews	Gene locus BU033 in contig BA000003_GR.
KEGG	buc:BU033.
PATRIC	21243572. VBIBucAph127364_0046.
Phylogenomic databases
GeneTree	EBGT00050000008021.
HOGENOM	HBG621785.
OMA	DINDKHI.
PhylomeDB	P57145.
ProtClustDB	PRK00566.
Enzyme and pathway databases
BioCyc	BSP107806:BU033-MONOMER.
Family and domain databases
HAMAP	MF_01322. RNApol_bact_RpoC. [Tree]
InterPro	IPR013328. DH_multihelical. IPR012754. DNA-dir_RpoC_beta_prime. IPR000722. RNA_pol_asu. IPR006592. RNA_pol_N. IPR007080. RNA_pol_Rpb1_1. IPR007066. RNA_pol_Rpb1_3. IPR007083. RNA_pol_Rpb1_4. IPR007081. RNA_pol_Rpb1_5. [Graphical view]
Gene3D	G3DSA:1.10.1040.10. Opine_DH. 1 hit.
KO	K03046.
Pfam	PF04997. RNA_pol_Rpb1_1. 1 hit. PF00623. RNA_pol_Rpb1_2. 1 hit. PF04983. RNA_pol_Rpb1_3. 1 hit. PF05000. RNA_pol_Rpb1_4. 1 hit. PF04998. RNA_pol_Rpb1_5. 1 hit. [Graphical view]
SMART	SM00663. RPOLA_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR02386. RpoC_TIGR. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RPOC_BUCAI

Accession

Primary (citable) accession number: P57145

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	January 25, 2012
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program