Reviewed,
UniProtKB/Swiss-Prot P57142 (METE_BUCAI)
Last modified
January 19, 2010.
Version 55.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
Customize display | text xml rdf/xml gff fasta |
Names and origin
| Protein names | Recommended name: 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase EC=2.1.1.14 Alternative name(s): Methionine synthase, vitamin-B12 independent isozyme Cobalamin-independent methionine synthase | ||||
| Gene names |
| ||||
| Organism | Buchnera aphidicola subsp. Acyrthosiphon pisum (Acyrthosiphon pisum symbiotic bacterium) [Complete proteome] [HAMAP] | ||||
| Taxonomic identifier | 118099 [NCBI] | ||||
| Taxonomic lineage | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera |
Protein attributes
| Sequence length | 758 AA. |
| Sequence status | Complete. |
| Protein existence | Inferred from homology. |
General annotation (Comments)
| Function | Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation By similarity. HAMAP MF_00172 |
| Catalytic activity | 5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine. HAMAP MF_00172 |
| Cofactor | Binds 1 zinc ion per subunit By similarity. HAMAP MF_00172 |
| Pathway | Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1. HAMAP MF_00172 |
| Sequence similarities | Belongs to the vitamin-B12 independent methionine synthase family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Amino-acid biosynthesis Methionine biosynthesis |
| Domain | Repeat |
| Ligand | Metal-binding Zinc |
| Molecular function | Methyltransferase Transferase |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | methionine biosynthetic process Inferred from electronic annotation. Source: HAMAP |
| Molecular function | 5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: HAMAP zinc ion bindingInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 758 | 758 | 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase HAMAP MF_00172 | PRO_0000098619 | |||||
Sites | |||||||||
| Metal binding | 639 | 1 | Zinc By similarity | ||||||
| Metal binding | 641 | 1 | Zinc By similarity | ||||||
| Metal binding | 724 | 1 | Zinc By similarity | ||||||
Sequences
| ||||||||||||||||||
References
| [1] | "Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed: 10993077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tokyo 1998. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | BA000003 Genomic DNA. Translation: BAB12757.1. |
| RefSeq | NP_239871.1. |
3D structure databases | |
| SMR | P57142. Positions 5-751. |
| ModBase | Search... |
Genome annotation databases | |
| GeneID | 1109538. |
| GenomeReviews | Gene locus BU030 in contig BA000003_GR. |
| KEGG | buc:BU030. |
Organism-specific databases | |
| CMR | Search... |
Phylogenomic databases | |
| HOGENOM | HBG287495. |
| OMA | SFVRNDI. |
| PhylomeDB | P57142. |
Enzyme and pathway databases | |
| BioCyc | BSP107806:BU030-MONOMER. |
Family and domain databases | |
| HAMAP | MF_00172. Meth_synth. [Tree] |
| InterPro | IPR013215. Cbl-indep_Met_Synth_N. IPR002629. Methionine_synth. IPR006276. MeTrfase_B12_ind. [Graphical view] |
| Pfam | PF08267. Meth_synt_1. 1 hit. PF01717. Meth_synt_2. 1 hit. [Graphical view] |
| PIRSF | PIRSF000382. MeTrfase_B12_ind. 1 hit. |
| TIGRFAMs | TIGR01371. met_syn_B12ind. 1 hit. |
| ProtoNet | Search... |
Entry information
| Entry name | METE_BUCAI | ||||||||
| Accession | Primary (citable) accession number: P57142 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes) | ||||||||
Relevant documents
| Buchnera aphidicola (subsp. Acyrthosiphon pisum) Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

Clusters with


