Reviewed, UniProtKB/Swiss-Prot P57142 (METE_BUCAI)

Names and origin

Protein names

Recommended name:
    5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
    EC=2.1.1.14
Alternative name(s):
    Methionine synthase, vitamin-B12 independent isozyme
    Cobalamin-independent methionine synthase

Gene names

Name:	metE
Ordered Locus Names:	BU030

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (Acyrthosiphon pisum symbiotic bacterium) [Complete proteome] [HAMAP]

Taxonomic identifier

118099 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	758 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation By similarity. HAMAP MF_00172
Catalytic activity	5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine. HAMAP MF_00172
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00172
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1. HAMAP MF_00172
Sequence similarities	Belongs to the vitamin-B12 independent methionine synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Domain	Repeat
Ligand	Metal-binding Zinc
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 758	758	5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase HAMAP MF_00172		PRO_0000098619
Sites
	Metal binding	639	1	Zinc By similarity
	Metal binding	641	1	Zinc By similarity
	Metal binding	724	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	P57142-1 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: 20F1C5160DF46D24	FASTA	758	87,916
	10 20 30 40 50 60 MTILNHTLGF PRIGLNRELK KAQEQYWSGE LMIKDLLLVG SELRKKNWQK QKESGIDYIP 70 80 90 100 110 120 VGDFAWYDHV LTTSMMLGNI PERHNNTVDS IDLDCLFRIA RGCPPDISAS EMTKWFNTNY 130 140 150 160 170 180 HYIVPEFYKN KVLKYSWKQI LDEVDEALLL GHKVKPILLG PITYLWLGKV KGEYFDRLDI 190 200 210 220 230 240 LKDIILIYKH VLKELSNRSI DFVQIDEPVL VLELPKKWKD AYHYAYKELS GITKLLLTTY 250 260 270 280 290 300 FDSIEHNIEF IRDLPVQGIH IDLVHGKYNL KNFSSKIPSE WMLSLGVING RNIWRSDLLK 310 320 330 340 350 360 WFKSIKSISN HHRKILIGSS CSLLHTPIDL VAEKHLDKEV KRWFSFAVQK CEELRLLSSA 370 380 390 400 410 420 LNDNDIDSIK EWSLPIYERS VSKRVNKIEV ENRLSNVLID KHQRLSPYKT RSIEQNKKFN 430 440 450 460 470 480 FPILPTTTIG SFPQTISIRK LRRDFKLGLV TEEEYTKIIK KNIKKVIKIQ EELDIDVLVH 490 500 510 520 530 540 GEAERNDMVE YFGEHLDGFA FTDNGWVQSY GSRCVKPPII IGDISRPKPM TIEWSKYAQS 550 560 570 580 590 600 LTKKPVKGML TGPVTILLWS FPREDVSLKK IATQIALALY DEVLDLEKEK IEIIQIDEPA 610 620 630 640 650 660 LREGLPLRKS SWHEYLSWAV DVFRLSASGV KNTTQIHTHM CYCEFNDIMD SIALLDADVI 670 680 690 700 710 720 TIEAARSDME LLESFKKFKY PNEVGPGAYD IHSSNIPSVQ SIISLLNKAM KYIPLKRIWV 730 740 750 NPDCGLKTRN WNETILSLKN MVEATKILRE KMKDSECD

References

[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed: 10993077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tokyo 1998.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000003 Genomic DNA. Translation: BAB12757.1.
RefSeq	NP_239871.1.
3D structure databases
SMR	P57142. Positions 5-751.
ModBase	Search...
Genome annotation databases
GeneID	1109538.
GenomeReviews	Gene locus BU030 in contig BA000003_GR.
KEGG	buc:BU030.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG287495.
OMA	SFVRNDI.
PhylomeDB	P57142.
Enzyme and pathway databases
BioCyc	BSP107806:BU030-MONOMER.
Family and domain databases
HAMAP	MF_00172. Meth_synth. [Tree]
InterPro	IPR013215. Cbl-indep_Met_Synth_N. IPR002629. Methionine_synth. IPR006276. MeTrfase_B12_ind. [Graphical view]
Pfam	PF08267. Meth_synt_1. 1 hit. PF01717. Meth_synt_2. 1 hit. [Graphical view]
PIRSF	PIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMs	TIGR01371. met_syn_B12ind. 1 hit.
ProtoNet	Search...

Entry information

Entry name

METE_BUCAI

Accession

Primary (citable) accession number: P57142

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	January 19, 2010
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)