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P57139

- GLMU_BUCAI

UniProt

P57139 - GLMU_BUCAI

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Protein

Bifunctional protein GlmU

Gene
glmU, BU027
Organism
Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP-GlcNAc). The C-terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N-acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5-monophosphate (from uridine 5-triphosphate), a reaction catalyzed by the N-terminal domain By similarity.UniRule annotation

Catalytic activityi

Acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + N-acetyl-alpha-D-glucosamine 1-phosphate.UniRule annotation
UTP + N-acetyl-alpha-D-glucosamine 1-phosphate = diphosphate + UDP-N-acetyl-alpha-D-glucosamine.UniRule annotation

Cofactori

Binds 1 magnesium ion per subunit By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei25 – 251UDP-GlcNAc By similarity
Binding sitei76 – 761UDP-GlcNAc By similarity
Metal bindingi105 – 1051Magnesium By similarity
Binding sitei140 – 1401UDP-GlcNAc; via amide nitrogen By similarity
Binding sitei154 – 1541UDP-GlcNAc By similarity
Metal bindingi227 – 2271Magnesium By similarity
Binding sitei227 – 2271UDP-GlcNAc By similarity
Binding sitei333 – 3331Acetyl-CoA; amide nitrogen By similarity
Binding sitei351 – 3511Acetyl-CoA By similarity
Active sitei363 – 3631Proton acceptor By similarity
Binding sitei366 – 3661Acetyl-CoA By similarity
Binding sitei377 – 3771Acetyl-CoA By similarity
Binding sitei405 – 4051Acetyl-CoA By similarity
Binding sitei423 – 4231Acetyl-CoA; via amide nitrogen By similarity

GO - Molecular functioni

  1. glucosamine-1-phosphate N-acetyltransferase activity Source: UniProtKB-HAMAP
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. UDP-N-acetylglucosamine diphosphorylase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. cell morphogenesis Source: UniProtKB-HAMAP
  2. lipid A biosynthetic process Source: UniProtKB-UniPathway
  3. lipopolysaccharide biosynthetic process Source: InterPro
  4. peptidoglycan biosynthetic process Source: UniProtKB-HAMAP
  5. regulation of cell shape Source: UniProtKB-KW
  6. UDP-N-acetylglucosamine biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Nucleotidyltransferase, Transferase

Keywords - Biological processi

Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciBAPH107806:GBZJ-27-MONOMER.
UniPathwayiUPA00113; UER00532.
UPA00113; UER00533.
UPA00973.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional protein GlmU
Including the following 2 domains:
UDP-N-acetylglucosamine pyrophosphorylase (EC:2.7.7.23)
Alternative name(s):
N-acetylglucosamine-1-phosphate uridyltransferase
Glucosamine-1-phosphate N-acetyltransferase (EC:2.3.1.157)
Gene namesi
Name:glmU
Ordered Locus Names:BU027
OrganismiBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Taxonomic identifieri107806 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
ProteomesiUP000001806: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 459459Bifunctional protein GlmUUniRule annotationPRO_0000068698Add
BLAST

Interactioni

Subunit structurei

Homotrimer By similarity.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliP57139.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 229229Pyrophosphorylase By similarityAdd
BLAST
Regioni11 – 144UDP-GlcNAc binding By similarity
Regioni81 – 822UDP-GlcNAc binding By similarity
Regioni103 – 1053UDP-GlcNAc binding By similarity
Regioni230 – 25021Linker By similarityAdd
BLAST
Regioni251 – 459209N-acetyltransferase By similarityAdd
BLAST
Regioni386 – 3872Acetyl-CoA binding By similarity

Sequence similaritiesi

In the N-terminal section; belongs to the N-acetylglucosamine-1-phosphate uridyltransferase family.
In the C-terminal section; belongs to the transferase hexapeptide repeat family.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG1207.
HOGENOMiHOG000283476.
KOiK04042.
OMAiDCVTNQD.
OrthoDBiEOG6Z6FQZ.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
HAMAPiMF_01631. GlmU.
InterProiIPR005882. Bifunctional_GlmU.
IPR001451. Hexapep_transf.
IPR018357. Hexapep_transf_CS.
IPR025877. MobA-like_NTP_Trfase_dom.
IPR029044. Nucleotide-diphossugar_trans.
IPR011004. Trimer_LpxA-like.
[Graphical view]
PfamiPF00132. Hexapep. 3 hits.
PF12804. NTP_transf_3. 1 hit.
[Graphical view]
SUPFAMiSSF51161. SSF51161. 1 hit.
SSF53448. SSF53448. 1 hit.
TIGRFAMsiTIGR01173. glmU. 1 hit.
PROSITEiPS00101. HEXAPEP_TRANSFERASES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P57139-1 [UniParc]FASTAAdd to Basket

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MLTQEIIIVI LAAGKGTRMK SNHPKVLHFL GGKTILEHVI ETAQSIKPKK    50
IILVYSDQKK PVLSNIYNIP IQWIIQKKPQ GTGHAILLAI KKISDNTEIL 100
VLYGDVPFIS PVSIKKLQKS KKQSKISLLT AKVKNPNGYG RILRKKGKVI 150
SIIEDQDASN EQKNIKEIYS GIFIAQSKDL TRWLKKIDKK NEKQEFYATD 200
IIALAHLEGS FIKTIEPLNY EEILGINNKL QLSNLEKIFQ KKQINKLLIN 250
GVTIKDPSHF IFRGTLQHGQ NVEIDTGVIL ENNVILGDDV KIGPGCIIRN 300
SSIDSNTNIQ AYTIIENSKI GKGCIIGPFA HLRSNTLLDR NVHIGNFVET 350
KDTFIKNESK VKHLSYLGNS EIGSKVNIGA GSITCNYDGA NKFKTIIGDN 400
VLVGSNTQLI APIKIAKNTT IAAGTTVTKD VNTPCLVYNT KEQKYKKNWM 450
RSKKIIKKN 459
Length:459
Mass (Da):51,182
Last modified:December 1, 2000 - v1
Checksum:iC26CAFBD3852AAF4
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000003 Genomic DNA. Translation: BAB12754.1.
RefSeqiNP_239868.1. NC_002528.1.

Genome annotation databases

EnsemblBacteriaiBAB12754; BAB12754; BAB12754.
GeneIDi1109535.
KEGGibuc:BU027.
PATRICi21243558. VBIBucAph127364_0039.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000003 Genomic DNA. Translation: BAB12754.1 .
RefSeqi NP_239868.1. NC_002528.1.

3D structure databases

ProteinModelPortali P57139.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAB12754 ; BAB12754 ; BAB12754 .
GeneIDi 1109535.
KEGGi buc:BU027.
PATRICi 21243558. VBIBucAph127364_0039.

Phylogenomic databases

eggNOGi COG1207.
HOGENOMi HOG000283476.
KOi K04042.
OMAi DCVTNQD.
OrthoDBi EOG6Z6FQZ.

Enzyme and pathway databases

UniPathwayi UPA00113 ; UER00532 .
UPA00113 ; UER00533 .
UPA00973 .
BioCyci BAPH107806:GBZJ-27-MONOMER.

Family and domain databases

Gene3Di 3.90.550.10. 1 hit.
HAMAPi MF_01631. GlmU.
InterProi IPR005882. Bifunctional_GlmU.
IPR001451. Hexapep_transf.
IPR018357. Hexapep_transf_CS.
IPR025877. MobA-like_NTP_Trfase_dom.
IPR029044. Nucleotide-diphossugar_trans.
IPR011004. Trimer_LpxA-like.
[Graphical view ]
Pfami PF00132. Hexapep. 3 hits.
PF12804. NTP_transf_3. 1 hit.
[Graphical view ]
SUPFAMi SSF51161. SSF51161. 1 hit.
SSF53448. SSF53448. 1 hit.
TIGRFAMsi TIGR01173. glmU. 1 hit.
PROSITEi PS00101. HEXAPEP_TRANSFERASES. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
    Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
    Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: APS.

Entry informationi

Entry nameiGLMU_BUCAI
AccessioniPrimary (citable) accession number: P57139
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: June 11, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. Buchnera aphidicola (subsp. Acyrthosiphon pisum)
    Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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