P57138 (GLMS_BUCAI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glucosamine--fructose-6-phosphate aminotransferase [isomerizing]
EC=2.6.1.16

Alternative name(s):
D-fructose-6-phosphate amidotransferase
GFAT
Glucosamine-6-phosphate synthase
Hexosephosphate aminotransferase
L-glutamine-D-fructose-6-phosphate amidotransferase

Gene names

Name:	glmS
Ordered Locus Names:	BU026

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)

Taxonomic identifier

107806 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	609 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the first step in hexosamine metabolism, converting fructose-6P into glucosamine-6P using glutamine as a nitrogen source By similarity. HAMAP MF_00164
Catalytic activity	L-glutamine + D-fructose 6-phosphate = L-glutamate + D-glucosamine 6-phosphate. HAMAP MF_00164
Subunit structure	Homodimer By similarity. HAMAP MF_00164
Subcellular location	Cytoplasm By similarity HAMAP MF_00164.
Sequence similarities	Contains 1 glutamine amidotransferase type-2 domain. Contains 2 SIS domains.
Sequence caution	The sequence BAB12753.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cytoplasm
Domain	Glutamine amidotransferase Repeat
Molecular function	Aminotransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	carbohydrate biosynthetic process Inferred from electronic annotation. Source: InterPro glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glutamine-fructose-6-phosphate transaminase (isomerizing) activity Inferred from electronic annotation. Source: EC sugar binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 609	608	Glucosamine--fructose-6-phosphate aminotransferase [isomerizing] HAMAP MF_00164		PRO_0000135311
Regions
	Domain	2 – 219	218	Glutamine amidotransferase type-2
	Domain	288 – 428	141	SIS 1
	Domain	460 – 599	140	SIS 2
Sites
	Active site	2	1	Nucleophile; for GATase activity By similarity
	Active site	604	1	For Fru-6P isomerization activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	P57138 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 5FF11730FEB2D715	FASTA	609	68,682
	10 20 30 40 50 60 MCGIVAAVTQ RNIANFLIDG IKKLEYRGYD SSGLAVIDNK NNIVRIRCVG KVNELIKKTN 70 80 90 100 110 120 KKKILGSIGV AHTRWATHGK VSKENTHPHI SSNIIVVHNG IIENNSTLRG FLKKQGYIFS 130 140 150 160 170 180 SDTDTEVIAH LLHWEQNKKK DSLIKVIQNS IKKLDGNYSM VVIDQNNPSK LIAARSGSPL 190 200 210 220 230 240 IIGLGTEENF IASDQIALLH VTKRFIYLEE GDIAIVARKE INIFNKNNSI IQREEVVSNI 250 260 270 280 290 300 EYKSAKKGKY RYYMEKEIHE QPKSIRNTLK NRLTNSNKVH FSELGSKENN IFYNTEHIQI 310 320 330 340 350 360 VACGTSYNAA MVSRYWFEEL ANIPCDVEIA SEFSSRKLVV RKKSLLITLS QSGETADTLS 370 380 390 400 410 420 ALRYSKKLGY LGNLTICNMK SSSLVRESDF YILTKAGLEI GVASTKSFTT QLTVLLLLVA 430 440 450 460 470 480 KIINSKKENN NTSKRIVQTL SILPVRIEEI LKKKQLIQDM ANTLANKKNM LFLGRGNQYP 490 500 510 520 530 540 IAMEGALKLK EISYIHAEAY PSGELKHGPL ALIDKNIPVI MIAPENSLLE KNKKNIKEIC 550 560 570 580 590 600 SRGGIVYVFS NQEFDYEENI NTIKLPYVEE LIAPIFYTIP LQLFAYYVAL KKGRDIDQPR HLAKSVTVE

References

[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed: 10993077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: APS.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000003 Genomic DNA. Translation: BAB12753.1. Different initiation.
RefSeq	NP_239867.2. NC_002528.1.
3D structure databases
ProteinModelPortal	P57138.
SMR	P57138. Positions 2-609.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBUCT00000002861; EBBUCP00000002637; EBBUCG00000002861.
GeneID	1109534.
GenomeReviews	Gene locus BU026 in contig BA000003_GR.
KEGG	buc:BU026.
PATRIC	21243556. VBIBucAph127364_0038.
Phylogenomic databases
GeneTree	EBGT00050000007826.
HOGENOM	HBG645312.
OMA	HIIRIPQ.
PhylomeDB	P57138.
ProtClustDB	PRK00331.
Enzyme and pathway databases
BioCyc	BSP107806:BU026-MONOMER.
Family and domain databases
HAMAP	MF_00164. GlmS. [Tree]
InterPro	IPR000583. GATase_2. IPR017932. GATase_II. IPR005855. GlmS_trans. IPR001347. SIS. [Graphical view]
KO	K00820.
Pfam	PF00310. GATase_2. 1 hit. PF01380. SIS. 2 hits. [Graphical view]
TIGRFAMs	TIGR01135. GlmS. 1 hit.
PROSITE	PS51278. GATASE_TYPE_2. 1 hit. PS51464. SIS. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLMS_BUCAI

Accession

Primary (citable) accession number: P57138

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	January 23, 2007
Last modified:	January 25, 2012
This is version 71 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program